Brain fluid calcium concentration and response to acute hypercalcaemia during development in the rat.

Jones, Hazel C.; Keep, Richard F.

doi:10.1113/jphysiol.1988.sp017223

Cited by 144 publications

(115 citation statements)

References 25 publications

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“…Neuromodulators can also influence this presynaptic mechanism, leading to transient changes in glutamatergic transmission (Gordon and Bains, 2005). Our data show that the switch from univesicular to multivesicular release at MF terminals only can be observed at lower (1.2 mM) Ca 2ϩ concentrations; this value is compatible with the estimated concentration of extracellular Ca 2ϩ in in vivo conditions (Jones and Keep, 1988;Chauvette et al, 2012), suggesting that an alteration in the number of vesicles released during AP firing can be a key element of short-term changes in synaptic strength. However, at higher extracellular calcium concentrations or in later segments of longer trains, multivesicular release was already operational and did not contribute to additional increases in glutamate release.…”

Section: Discussionsupporting

confidence: 68%

Interplay between Synchronization of Multivesicular Release and Recruitment of Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy Fiber to CA3 Pyramidal Cells Synapses

Chamberland

Evstratova

Tóth

2014

Journal of Neuroscience

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Synaptic short-term plasticity is a key regulator of neuronal communication and is controlled via various mechanisms. A well establishedproperty of mossy fiber to CA3 pyramidal cell synapses is the extensive short-term facilitation during high-frequency bursts. We investigated the mechanisms governing facilitation using a combination of whole-cell electrophysiological recordings, electrical minimal stimulation, and random-access two-photon microscopy in acute mouse hippocampal slices. Two distinct presynaptic mechanisms were involved in short-term facilitation, with their relative contribution dependent on extracellular calcium concentration.

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Section: Discussionsupporting

confidence: 68%

Interplay between Synchronization of Multivesicular Release and Recruitment of Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy Fiber to CA3 Pyramidal Cells Synapses

Chamberland

Evstratova

Tóth

2014

Journal of Neuroscience

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“…The composition of ACSF solutions may vary slightly between laboratories. For example, the calcium concentration is often two or three times higher than physiological levels (∼1.2 mM) (35)(36)(37), which artificially increases synaptic release and therefore may also increase calcium neural network activity. To address this issue, we matched the inorganic salt concentration, the pH, and osmolarity of our ACSF to those in DMEM and repeated the calcium-imaging experiments.…”

Section: Significancementioning

confidence: 99%

Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

Bardy

Hurk

Eames

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

343

363

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Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiological medium also improved the proportion of neurons that were synaptically active. The medium was designed to culture human neurons but also proved adequate for rodent neurons. The improvement in BrainPhys basal medium to support neurophysiological activity is an important step toward reducing the gap between brain physiological conditions in vivo and neuronal models in vitro.

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“…These [Ca 2ϩ ] o and [K ϩ ] o were chosen because they are close to those found in the CSF of adult rats (Fisher et al, 1976;Poolos et al, 1987;Jones and Keep, 1988).…”

Section: Methodsmentioning

confidence: 99%

On the Initiation and Propagation of Dendritic Spikes in CA1 Pyramidal Neurons

Gasparini¹,

Migliore²,

Magee³

2004

J. Neurosci.

316

343

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Under certain conditions, regenerative voltage spikes can be initiated locally in the dendrites of CA1 pyramidal neurons. These are interesting events that could potentially provide neurons with additional computational abilities. Using whole-cell dendritic recordings from the distal apical trunk and proximal tuft regions and realistic computer modeling, we have determined that highly synchronized and moderately clustered inputs are required for dendritic spike initiation: ϳ50 synaptic inputs spread over 100 m of the apical trunk/tuft need to be activated within 3 msec. Dendritic spikes are characterized by a more depolarized voltage threshold than at the soma [Ϫ48 Ϯ 1 mV (n ϭ 30) vs Ϫ56 Ϯ 1 mV (n ϭ 7), respectively] and are mainly generated and shaped by dendritic Na ϩ and K ϩ currents. The relative contribution of AMPA and NMDA currents is also important in determining the actual spatiotemporal requirements for dendritic spike initiation. Once initiated, dendritic spikes can easily reach the soma, but their propagation is only moderately strong, so that it can be modulated by physiologically relevant factors such as changes in the V m and the ionic composition of the extracellular solution. With effective spike propagation, an extremely short-latency neuronal output is produced for greatly reduced input levels. Therefore, dendritic spikes function as efficient detectors of specific input patterns, ensuring that the neuronal response to high levels of input synchrony is a precisely timed action potential output.

show abstract

Brain fluid calcium concentration and response to acute hypercalcaemia during development in the rat.

Cited by 144 publications

References 25 publications

Interplay between Synchronization of Multivesicular Release and Recruitment of Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy Fiber to CA3 Pyramidal Cells Synapses

Interplay between Synchronization of Multivesicular Release and Recruitment of Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy Fiber to CA3 Pyramidal Cells Synapses

Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

On the Initiation and Propagation of Dendritic Spikes in CA1 Pyramidal Neurons

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