Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish

Tseng, Yung‐Che; Liu, Sian Tai; Hu, Marian Yong-An; Chen, Ruo Dong; Lee, Jay Ron; Hwang, Pung Pung

doi:10.1186/s12983-014-0053-1

Cited by 26 publications

(14 citation statements)

References 82 publications

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“…The brain and heart transcriptome profiles of the control and cold-acclimated fish of different strains were characterized. Brain and heart were selected because their functionalities are sensitive to changes in environmental temperatures and are paramount for fish survival from acute hypothermal stress ( Tseng et al, 2014 ; Keen et al, 2017 ). The gene expression datasets were analyzed by functional enrichment analyses to extract insights into the cold-regulated biological processes and pathways.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

Long

et al. 2020

Front. Genet.

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Properly regulated transcriptional responses to environmental perturbations are critical for the fitness of fish. Although gene expression profiles in the tissues of common carp upon cold stress were previously characterized, the transcriptional programs underlying cold acclimation are still not well known. In this study, the ability of three common carp strains including Hebao red carp (HB), Songpu mirror carp (SPM) and Yellow river carp (YR) to establish cold resistance after acclimation to a mild hypothermia stress at 18°C for 24 h was confirmed by measurements of the critical thermal minimums (CTMin). The gene expression profiles of the brain and the heart from these strains under both control and cold-acclimated conditions were characterized with RNA-sequencing. The data of the three common carp strains with different genetic background were combined in the differential gene expression analyses to balance the effects of genetic diversity on gene expression. Marked effects of tissue origins on the cold-induced transcriptional responses were revealed by comparing the differentially expressed gene (DEG) lists of the two tissues. Functional categories including spliceosome and RNA splicing were highly enriched in the DEGs of both tissues. However, steroid biosynthesis was specifically enriched in DEGs of the brain and response to unfolded protein was solely enriched in DEGs of the heart. Consistent with the up-regulation of the genes involved in cholesterol biosynthesis, total cholesterol content of the brain was significantly increased upon cold stress. Moreover, cold-induced alternative splicing (AS) events were explored and AS of the rbmx (RNA-binding motif protein, X chromosome) gene was confirmed by real-time quantitative PCR. Finally, a core set of cold responsive genes (CRGs) were defined by comparative transcriptomic analyses. Our data provide insights into the transcriptional programs underlying cold acclimation of common carp and offer valuable clues for further investigating the genetic determinants for cold resistance of farmed fish.

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Section: Introductionmentioning

confidence: 99%

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

Long

et al. 2020

Front. Genet.

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“…Meanwhile, most pathways in liver were downregulated and indicated its function in energy conservation during spawning migration. The brain serves as the center of the nervous system in vertebrates and exerts a more comprehensive regulatory function than other tissues of perception system regulation, learning, and memory muscle activity, through which the organism responds to the changing environment [11,12]. Therefore, research on the influence of environmental factor variation on the brain transcriptome will be beneficial for revealing the comprehensive regulatory network that is formed during C. nasus migration.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

Wang

Tang

et al. 2020

BMC Genomics

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Background: Coilia nasus (C. nasus) is an important anadromous fish species that resides in the Yangtze River of China, and has high ecological and economical value. However, wild resources have suffered from a serious reduction in population, attributed to the over-construction of water conservancy projects, overfishing, and environmental pollution. The Ministry of Agriculture and Rural Affairs of the People's Republic of China has issued a notice banning the commercial fishing of wild C. nasus in the Yangtze River. Wild C. nasus populations urgently need to recover. A better understanding of C. nasus migration patterns is necessary to maximize the efficiency of conservation efforts. Juvenile C. nasus experience a simultaneous effect of increasing salinity and cold stress during seaward migration, and the brain plays a comprehensive regulatory role during this process. Therefore, to explore the early seaward migration regulation mechanism of juvenile C. nasus, we performed a comparative transcriptome analysis on the brain of juvenile C. nasus under salinity and cold stress simultaneously. Results: Relevant neurotransmitters, receptors, and regulatory proteins from three categories of regulatory pathway play synergistic regulatory roles during the migration process: neuronal signaling, the sensory system, and environmental adaptation. The significant differential expression of growth-related hormones, thyroid receptors, haptoglobin, and prolactin receptors was similar to the results of relevant research on salmonids and steelhead trout. Conclusions: This study revealed a regulatory network that the brain of juvenile C. nasus constructs during migration, thereby providing basic knowledge on further studies could build on. This study also revealed key regulatory genes similar to salmonids and steelhead trout, thus, this study will lay a theoretical foundation for further study on migration regulation mechanism of anadromous fish species.

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“…Here, a 10°C decrease in temperature resulted in a 1.8- to 3.0-fold reduction in luminal

secretion in isolated intestine epithelia of gulf toadfish ( Opsanus beta ) (Grosell and Genz, 2006 ). Here, a proper metabolic shift fueled through alternative energy sources (e.g., lactate) under hypothermic stress has been demonstrated to be beneficial to counteract activity losses, thus maintaining intact homeostasis in zebrafish (Tseng et al, 2014 ). To date, combined effects of temperature and CO 2 on the intestinal pH regulatory machinery, are still poorly investigated for marine teleosts including Atlantic cod, Gadus morhua .…”

Section: Introductionmentioning

confidence: 99%

Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua

Michael

Kreiss

et al. 2016

Front. Physiol.

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CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for 4 weeks to three CO2 levels (550, 1200, and 2200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3− cotransporter (NBC1), pendrin-like Cl−/HCO3− exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA), and Cl− channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3− secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3− levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans.

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Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish

Cited by 26 publications

References 82 publications

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

Transcriptional Programs Underlying Cold Acclimation of Common Carp (Cyprinus carpio L.)

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

Temperature Modulates the Effects of Ocean Acidification on Intestinal Ion Transport in Atlantic Cod, Gadus morhua

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