Brain Microdialysis

McLawhorn, Marc; James, Michael L.

doi:10.1016/b978-0-12-809915-5.00008-5

Cited by 1 publication

(2 citation statements)

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“…However, in vivo MD is currently considered as the “gold standard” method for investigations regarding brain function. In general, the MD extracting phase consists of a semipermeable membrane filled with a circulating perfusate solution resembling the composition of the physiological fluid found at the site of the system under study . In this method, the gradient in analyte concentration created between the sample and perfusate solution becomes the driving force for extraction of metabolites, and selectivity is achieved by selecting the molecular weight cutoff size of the biocompatible membrane used in the construction of the MD probe.…”

Section: Introductionmentioning

confidence: 99%

“…In general, the MD extracting phase consists of a semipermeable membrane filled with a circulating perfusate solution resembling the composition of the physiological fluid found at the site of the system under study. 12 In this method, the gradient in analyte concentration created between the sample and perfusate solution becomes the driving force for extraction of metabolites, and selectivity is achieved by selecting the molecular weight cutoff size of the biocompatible membrane used in the construction of the MD probe. The dimensions of a typical MD probe are relatively small (1−4 mm in length and 200−400 μm in diameter), 13 enabling its applicability for low invasive explorations.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Investigation of Metabolic Changes Occurring in the Rat Brain Hippocampus after Fluoxetine Administration Using Two Complementary In Vivo Techniques: Solid Phase Microextraction and Microdialysis

Boyacı

Lendor

Bojko

et al. 2020

ACS Chem. Neurosci.

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Fluoxetine is among the most prescribed antidepressant drugs worldwide. Nevertheless, limited information is known about its definitive mechanism. Although in vivo examinations performed directly in related brain structures can provide more realistic, and therefore more insightful, knowledge regarding the mechanisms and efficacy of this drug, only a few techniques are applicable for in vivo monitoring of metabolic alterations in the brain following an inducement. Among them, solid phase microextraction (SPME) and microdialysis (MD) have emerged as ideal in vivo tools for extraction of information from biosystems. In this investigation, we scrutinized the capabilities of SPME and MD to detect ongoing changes in the brain following acute fluoxetine administration. Sequential in vivo samples were collected simultaneously from male rats' hippocampi using SPME and MD before drug administration in order to establish a baseline; then samples were collected again following fluoxetine administration for an investigation of small molecule alterations. Our results indicate that MD provides more comprehensive information for polar compounds, while SPME provides superior information with respect to lipids and other medium level polar molecules. Interestingly, in the lipidomic investigation, all dysregulated features were found to be membrane lipids and associated compounds. Moreover, in the metabolomic investigations, dysregulation of hippocampal metabolite levels associated with fatty acid transportation and purine metabolisms were among the most notable findings. Overall, our evaluation of the obtained data corroborates that, when used in tandem, SPME and MD are capable of providing comprehensive information regarding the effect of fluoxetine in targeted brain structures and further elucidating this drug's mechanisms of action in the brain.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%