Brain monoaminergic and neuropeptidergic variations in human aging

Arranz, Belén; Blennow, Kaj; Ekman, Rolf; Eriksson, Anders; Månsson, Jan‐Eric; Marcusson, Jan

doi:10.1007/bf01292620

Cited by 40 publications

(19 citation statements)

References 63 publications

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“…Hypothalamic noradrenaline content has been reported as reduced (Simpkins 1984;Roubein et al 1986) or unchanged (Petkov et al 1987) in aged animals. A recent study in humans reported significant reductions in hypothalamic noradrenaline concentration between 16 to 75 years of age (Arranz et al 1996). Similar developmental changes in whole brain dopamine content have been reported (Coyle 1973;Herregodts et al 1990).…”

Section: J a Hastings · J M Mcclure-sharp · M J Morrissupporting

confidence: 53%

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Hastings

McClure-Sharp

Morris

1998

Naunyn-Schmiedeberg's Arch Pharmacol

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Marked changes in brain monoamine content and NPY content occur during maturation and ageing. Earlier in vivo studies in our laboratory have reported blunted K+ stimulated noradrenaline release and reduced NPY overflow in aged animals using microdialysis and push pull techniques. In this study, in vitro superfusion techniques were established to measure endogenous noradrenaline, NPY, DOPAC and 5-HIAA overflow from the hypothalamus of 1, 5 and 16 month old Sprague-Dawley rats. A period of high K+ (56 mM) stimulation was carried out to elicit maximal release. Basal noradrenaline overflow was similar in all age groups of rats and during K+-induced depolarisation similar 3-4 fold increases were observed. On the other hand, basal and K+ stimulated NPY overflow were significantly greater in the adult rats compared to 1 month and 16 month old rats. Despite differences in absolute NPY overflow, the relative increase over resting was not significantly different across age groups. The molar quantities of hypothalamic NPY overflow at rest and under K+ stimulated conditions were three orders of magnitude lower than noradrenaline. Results of these studies suggest that both NPY and noradrenaline can be released from a similar hypothalamic pool. Basal and K+-evoked DOPAC and 5-HIAA overflow were similar between the 3 age groups. Thus the overflow of hypothalamic noradrenaline, DOPAC and 5-HIAA under in vitro conditions was not altered from 1 to 16 months. In contrast, 5 month old rats had significantly higher NPY overflow than the other age groups (P < 0.05), consistent with a reported decline in NPY content with advanced age. Hypothalamic noradrenaline overflow was not affected by ageing, suggesting that a selective loss of NPY in the arcuo-PVN projection, or other projections to the hypothalamus with ageing may contribute to the reduction in NPY overflow in aged rats.

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Section: J a Hastings · J M Mcclure-sharp · M J Morrissupporting

confidence: 53%

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Hastings

McClure-Sharp

Morris

1998

Naunyn-Schmiedeberg's Arch Pharmacol

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“…Factors from the ante-mortem period -in particular, the agonal state and the rapidity of death -as well as the method of tissue preparation and storage, may play greater roles in determining post-mortem yields of nucleic acids and proteins (Yates et al 1990;Harrison et al 1991Harrison et al , 1995Kingsbury et al 1995). While it is now generally accepted that PMI may critically affect the preservation of some brain neuropeptides and catecholamines (Dodd et al 1988;Palmer et al 1988a;Arranz et al 1996), many nucleic acids and proteins are remarkably stable post-mortem (Lukiw et al 1990;Yates et al 1990;Harrison et al 1991;Leonard et al 1993;Ludes et al 1993;Johnston et al 1997;Schramm et al 1999;Yasojima et al 2001;Bahn et al 2002). Similarly, while agonal state does not significantly affect the overall autopsy concentrations of RNA or proteins, it can influence specific transcripts and proteins (Harrison et al 1991).…”

mentioning

confidence: 99%

Biochemical and molecular studies using human autopsy brain tissue

Hynd

Lewohl

Scott

et al. 2003

Journal of Neurochemistry

222

171

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The use of human brain tissue obtained at autopsy for neurochemical, pharmacological and physiological analyses is reviewed. RNA and protein samples have been found suitable for expression profiling by techniques that include RT-PCR, cDNA microarrays, western blotting, immunohistochemistry and proteomics. The rapid development of molecular biological techniques has increased the impetus for this work to be applied to studies of brain disease. It has been shown that most nucleic acids and proteins are reasonably stable postmortem. However, their abundance and integrity can exhibit marked intra-and intercase variability, making comparisons between case-groups difficult. Variability can reveal important functional and biochemical information. The correct interpretation of neurochemical data must take into account such factors as age, gender, ethnicity, medicative history, immediate ante-mortem status, agonal state and post-mortem and post-autopsy intervals. Here we consider issues associated with the sampling of DNA, RNA and proteins using human autopsy brain tissue in relation to various ante-and postmortem factors. We conclude that valid and practical measures of a variety of parameters may be made in human brain tissue, provided that specific factors are controlled.

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“…Aging is often accompanied by some alterations in the neurotransmitter systems of humans and other mammals (Arranz et al, 1996;Magnone et al, 2000;Monica et al, 2003). Most of the studies on brain physiology in aging have been performed in rodents and the results do not always show consistent changes in the neurochemical parameters.…”

Section: Discussionmentioning

confidence: 99%

Ameliorating effects of essential oil from Acori graminei rhizoma on learning and memory in aged rats and mice

Zhang

Han

et al. 2007

Journal of Pharmacy and Pharmacology

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Althouh there are normal cognitive changes that take place when a person becomes older, aging in humans is generally associated with deterioration of cognitive performance and, in particular, of learning and memory. These cognitive deficits can cause debilitating consequences due to aging. There are a number of herbal medicines which are reported to improve brain function including intelligence. In the present study, ameliorating effects of essential oil (EO), extracted from Acori graminei rhizoma (AGR), on learning and memory in aged, dysmnesia rats and mice were determined by the use of step-down type passive avoidance test or Y type maze trial. Oral administration of EO (0.02, 0.04, 0.08 g/kg, in rats given for 30d and in mice for 15d) evidently improved the latency and number of errors in aged, dysmnesia rats and mice.The cerebral neurotransmitters in aged rats given EO (0.02, 0.04, 0.08 g/kg for 30d)were also investigated and, increased levels of norepinephrine (NE), dopamine (DA), serotonin (5-HT) and decreased levels of acetylcholine esterase (AChE) activity were found. These results suggest that EO improves cognitive function of aged animals and may do so by relatively increasing NE, DA, 5-HT levels and by decreasing the activity of AChE in the cerebra.

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Brain monoaminergic and neuropeptidergic variations in human aging

Cited by 40 publications

References 63 publications

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Biochemical and molecular studies using human autopsy brain tissue

Ameliorating effects of essential oil from Acori graminei rhizoma on learning and memory in aged rats and mice

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