Brain perfusion fixation in male pigs using a safer closed system

Musigazi, Gracia Umuhire; Vleeschauwer, Stéphanie De; Sciot, Raf; Verbeken, Eric; Depreitere, Bart

doi:10.1177/0023677217752747

Cited by 10 publications

(4 citation statements)

References 11 publications

(16 reference statements)

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“…The animals were euthanized 7 days after the second phase using an overdose of barbiturates (400 mg/ml; Euthasol, Le Vet, Oudewater, The Netherlands). The mandible and maxilla were simultaneously fixed by immersion in 4% paraformaldehyde solution (VWR) via a closed headonly perfusion system with inflow through the carotid artery and outflow via the jugular vein [28]. The teeth were removed individually from the jaw using an electric saw (HB 8894, HEBU Medical, Tuttlingen, Germany) under copious water irrigation and afterwards immediately fixed in 30 ml of 4% paraformaldehyde solution (VWR) at 4°C for 2 weeks.…”

Section: Introductionmentioning

confidence: 99%

Injectable phosphopullulan-functionalized calcium-silicate cement for pulp-tissue engineering: An in-vivo and ex-vivo study

Pedano

Santos

et al. 2020

Dental Materials

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ObjectivesTo evaluate, by means of an ex-vivo human tooth-culture model and in-vivo minipig animal study, the pulpal inflammatory reaction and reparative dentin-formation capacity of an injectable phosphopullulan-based calcium-silicate cement (GC, Tokyo, Japan) upon pulp capping, this in comparison with the commercial reference material Biodentine (Septodont). MethodsFor the ex-vivo tooth model, 9 freshly-extracted teeth from 3 different patients were pulp-capped with the experimental biomaterial (n=3), Biodentine (n=3) or left uncapped (control; n=3). The teeth were kept in fresh culture medium for 4 weeks and, upon fixation three-dimensional Micro-CT and histology were performed. For the in-vivo animal study, 40 teeth from 3 minipigs were exposed and pulp capped with the experimental biomaterial containing phosphopullulan (n=24) or Biodentine (n=16) for 7 or 70 days. The inflammatory reaction and the tissue-regenerative potential was qualitatively and semiquantitatively characterized using three-dimensional micro-CT and histology. ResultsEx vivo, the treatment with the experimental phosphopullulan-based calcium-silicate cement and Biodentine stimulated the formation of fibrous tissue and mineralized foci. In vivo, early inflammatory reaction and regeneration of the pulp-tissue interface was promoted by both bioceramic materials after 7 and 70 days, respectively. SignificanceOur findings bring new insights into calcium-silicate-mediated dental pulp repair and regeneration. The novel ready-to-use and self-adhering functionalized calcium-silicate cement revealed effective pulpal repair potential.

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Section: Introductionmentioning

confidence: 99%

Injectable phosphopullulan-functionalized calcium-silicate cement for pulp-tissue engineering: An in-vivo and ex-vivo study

Pedano

Santos

et al. 2020

Dental Materials

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“…Perfusion techniques in the literature, particularly with respect to fixation, predominately address small animal species, while few large animal perfusion techniques are available (Musigazi et al 2018). Due to the vast array of possible devices and implantation sites, discussions regarding the details of device-specific perfusion procedures or approaches are beyond the scope of this article; however, perfusion of fluids, particularly fixatives, is frequently required for device-treated tissues.…”

Section: Necropsy Procedures Planning and Personnelmentioning

confidence: 99%

An Overview on the Considerations for the Planning of Nonclinical Necropsies for Medical Device Studies

Stanley¹,

Keating²,

Souci³

2019

Toxicol Pathol

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The terminal collection and histological processing of medical devices is an expensive, labor-, and material-intensive endeavor, which requires adequate experience, innovation, and preparation for success. It is also an exciting endeavor that continually challenges, intellectually engages, and improves the skills and knowledge of the pathologist. Awareness of the importance of the medical device pathologist's involvement, communication, and oversight throughout the development, implementation, and execution of a nonclinical assessment of a medical device is in the best interest of the test facility, the histopathology laboratory, the pathologist, the sponsor, and, ultimately, the patients. This article serves to present as a primer of key considerations for the approach and conduct of "nontoxicological" studies, defined as studies involving animal models of deployment or implantation of medical devices as well as surgical animal models.

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“…Indeed, we found it easier to catheterize the carotid artery only, and not the jugular vein. Initially, the literature presents methods where the jugular vein is catheterized to drain waste20 . Practically, this limits the flow out of the brain and leads to poorer extraction of blood and the overall quality of perfusion.…”

mentioning

confidence: 99%

Subdural Soft Electrocorticography (ECoG) Array Implantation and Long-Term Cortical Recording in Minipigs

Fallegger

Trouillet

Lacour

2023

JoVE

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Neurological impairments and diseases can be diagnosed or treated using electrocorticography (ECoG) arrays. In drug-resistant epilepsy, these help delineate the epileptic region to resect. In long-term applications such as brain-computer interfaces, these epicortical electrodes are used to record the movement intention of the brain, to control the robotic limbs of paralyzed patients. However, current stiff electrode grids do not answer the need for high-resolution brain recordings and longterm biointegration. Recently, conformable electrode arrays have been proposed to achieve long-term implant stability with high performance. However, preclinical studies for these new implant technologies are needed to validate their long-term functionality and safety profile for their translation to human patients. In this context, porcine models are routinely employed in developing medical devices due to their large organ sizes and easy animal handling. However, only a few brain applications are described in the literature, mostly due to surgery limitations and integration of the implant system on a living animal.Here, we report the method for long-term implantation (6 months) and evaluation of soft ECoG arrays in the minipig model. The study first presents the implant system, consisting of a soft microfabricated electrode array integrated with a magnetic resonance imaging (MRI)-compatible polymeric transdermal port that houses instrumentation connectors for electrophysiology recordings. Then, the study describes the surgical procedure, from subdural implantation to animal recovery. We focus on the auditory cortex as an example target area where evoked potentials are induced by acoustic stimulation. We finally describe a data acquisition sequence that includes MRI of the whole brain, implant electrochemical characterization,

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Brain perfusion fixation in male pigs using a safer closed system

Cited by 10 publications

References 11 publications

Injectable phosphopullulan-functionalized calcium-silicate cement for pulp-tissue engineering: An in-vivo and ex-vivo study

Injectable phosphopullulan-functionalized calcium-silicate cement for pulp-tissue engineering: An in-vivo and ex-vivo study

An Overview on the Considerations for the Planning of Nonclinical Necropsies for Medical Device Studies

Subdural Soft Electrocorticography (ECoG) Array Implantation and Long-Term Cortical Recording in Minipigs

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