Brain Tissue Autofluorescence: An Aid for Intraoperative Delineation of Tumor Resection Margins

Bottiroli, Giovanni; Croce, Anna Cleta; Locatelli, D.; Nano, Rosanna; Giombelli, Ermanno; Messina, Alberto Luca; Benericetti, E.

doi:10.1046/j.1525-1500.1998.cdoa34.x

Cited by 60 publications

(50 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TFEAF investigations in animal models and biopsy tissues may also be valuable in the development and verification of optical tumour diagnosis based on large field autofluorescence imaging or spectral resolved detection of backscattered light [4,20,21].…”

Section: Discussionmentioning

confidence: 99%

Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density

Kantelhardt

Leppert

Kantelhardt

et al. 2009

Acta Neurochir (Wien)

View full text Add to dashboard Cite

Background Recent studies have highlighted the importance of the complete resection of a brain tumour but the task often remains a challenge for the neurosurgeon. New technologies which add objective information beyond visualisation provided by the traditional operating microscope are required. In this study, we have analysed the cellular density of the tumour/brain interface using three dimensional multi-photon microscopy intensity-images of experimental gliomas and human brain-tumour biopsy samples. Methods The density of cellular nuclei was determined in specimens of experimental gliomas in a mouse model and human brain tumour biopsies by analysis of optical tissue sections. Three dimensional multi-photon microscopy image stacks were compared to serial H&E stained sections of conventional histolopathology. Findings Both techniques consistently showed a good correlation of cell density values in solid tumour tissue of experimental gliomas versus adjacent brain. The multiphoton microscopy analysis of human biopsy specimens showed that optical analysis of native tissue provided information on the cellular density. Conclusions Multi-photon microscopy is an efficient and rapid tool for the study of brain and brain tumour tissue. Multi-photon microscopy allows the detection of individual tumour cells and tumour cell clusters in native tissue biopsies and may therefore provide a tool in the identification of highly cellular lesions during the resection of brain tumours.

show abstract

Section: Discussionmentioning

confidence: 99%

Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density

Kantelhardt

Leppert

Kantelhardt

et al. 2009

Acta Neurochir (Wien)

View full text Add to dashboard Cite

show abstract

“…For instance, fluorescence signals were lower in gliomas than in normal brain tissue, and flavin and porphyrin fluorescence in neoplastic tissues was different from that of normal tissue. Bottiroli et al 62 first reported an in vivo study on a limited number of patients that showed that brain tissue can be differentiated by its autofluorescence properties, including the emission peak and shape of fluorescence spectra, when excited at 366 nm. Lin et al reported a discrimination algorithm based on the ratio of the fluorescence emission and reflectance intensity at 625 nm.…”

Section: Steady-state Fluorescence Spectroscopymentioning

confidence: 99%

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Vasefi

MacKinnon²,

Farkas

et al. 2016

Neurophoton

View full text Add to dashboard Cite

“…Several research groups have demonstrated the feasibility of using intrinsic fluorescence and optical characteristics to differentiate brain tumors from normal brain tissue in vivo over the past decade. 1,5,10,[21][22][23][24]47,51 In a recent clinical trial, the Vanderbilt group demonstrated high sensitivity with a combined optical spectroscopic method to detect the infiltrating brain tumor margins. 47 The feasibility of using intraoperative optical spectroscopy to detect and demarcate epileptic lesions has been investigated on only very few occasions.…”

Section: Optical Spectroscopymentioning

confidence: 99%

The role of optical spectroscopy in epilepsy surgery in children

Bhatia

Ragheb

Johnson

et al. 2008

FOC

View full text Add to dashboard Cite

Object Surgery is an important therapeutic modality for pediatric patients with intractable epilepsy. However, existing imaging and diagnostic technologies such as MR imaging and electrocochleography (ECoG) do not always effectively delineate the true resection margin of an epileptic cortical lesion because of limitations in their sensitivity. Optical spectroscopic techniques such as fluorescence and diffuse reflectance spectroscopy provide a nondestructive means of gauging the physiological features of the brain in vivo, including hemodynamics and metabolism. In this study, the authors investigate the feasibility of using combined fluorescence and diffuse reflectance spectroscopy to assist epilepsy surgery in children. Methods In vivo static fluorescence and diffuse reflectance spectra were acquired from the brain in children undergoing epilepsy surgery. Spectral measurements were obtained using a portable spectroscopic system in conjunction with a fiber optic probe. The optical investigations were conducted at the normal and abnormal cortex as defined by intraoperative ECoG and preoperative imaging studies. Biopsy samples were taken from the investigated sites located within the zone of resection. The optical spectra were classified into multiple subsets in accordance with the ECoG and histological study results. The authors used statistical comparisons between 2 given data subsets to identify unique spectral features. Empirical discrimination algorithms were developed using the identified spectral features to determine if the objective of the study was achieved. Results Fifteen pediatric patients were enrolled in this pilot study. Elevated diffuse reflectance signals between 500 and 600 nm and/or between 650 and 850 nm were observed commonly in the investigated sites with abnormal ECoG and/or histological features in 10 patients. The appearance of a fluorescent peak at 400 nm was observed in both normal and abnormal cortex of 5 patients. These spectral alterations were attributed to changes in morphological and/or biochemical characteristics of the epileptic cortex. The sensitivities and specificities of the empirical discrimination algorithms, which were constructed using the identified spectral features, were all > 90%. Conclusions The results of this study demonstrate the feasibility of using static fluorescence and diffuse reflectance spectroscopy to differentiate normal from abnormal cortex on the basis of intraoperative assessment of ECoG and histological features. It is therefore possible to use fluorescence and diffuse reflectance spectroscopy as an aid in epilepsy surgery.

show abstract

Brain Tissue Autofluorescence: An Aid for Intraoperative Delineation of Tumor Resection Margins

Cited by 60 publications

References 20 publications

Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density

Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

The role of optical spectroscopy in epilepsy surgery in children

Contact Info

Product

Resources

About