Brain Tumors in Owl Monkeys Inoculated with a Human Polyomavirus (JC Virus)

London, William T.; Houff, Sidney A.; Madden, David L.; Fuccillo, David A.; Gravell, Maneth; Wallen, William C.; Palmer, Amos E.; Sever, John L.; Padgett, Billie L.; Walker, Duard L.; ZuRhein, Gabriele M.; Ohashi, Tomoo

doi:10.1126/science.211583

Cited by 203 publications

(105 citation statements)

References 14 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…The majority of the monkeys were inoculated intracerebrally as adults, and a small subset received immunosuppressive drugs prior to and following inoculation to aid in tumor inducement (London et al, 1978). Several of the infected animals, with and without immunosuppression, developed glial neoplasias in adulthood, including glioblastoma multiforme and astrocytoma.…”

Section: Laboratory Animal Studiesmentioning

confidence: 99%

Human neurotropic polyomavirus, JCV, and its role in carcinogenesis

et al. 2003

View full text Add to dashboard Cite

A number of recent studies have reported the detection of the ubiquitous human polyomavirus, JC virus (JCV), in samples derived from several types of neural as well as non-neural human tumors. The human neurotropic JCV was first identified as the etiologic agent of the fatal demyelinating disease, progressive multifocal leukoencephalopathy, which usually occurs in individuals with defects in cell-mediated immunity, including AIDS. However, upon mounting evidence of the oncogenic potential of the viral regulatory protein, T-antigen, and JCV's oncogenecity in a broad range of animal models, studies were initiated to determine its potential involvement in human carcinogenesis. Initially, the most frequently observed tumors in rodent models, including medulloblastoma, astrocytoma, glioblastoma, and other neural-origin tumors were analysed. These studies were followed by analysis of non-neural tumors such as colorectal carcinomas. In a subset of each tumor type examined, JC viral genomic DNA sequences could be detected by PCR and confirmed by Southern blot hybridization or direct sequencing. In a smaller subset of the tumors, the expression of T-antigen was observed by immunohistochemical analysis. Owing to the established functions of T-antigen including its ability to interact with tumor suppressor proteins such as Rb and p53, and its ability to influence chromosomal stability, potential mechanisms of JCV T-antigen-mediated cellular dysregulation are discussed. Further, as increasing evidence suggests that T-antigen is not required for maintenance of a transformed phenotype, a hit-and-run model for T-antigen-induced transformation is proposed.

show abstract

Section: Laboratory Animal Studiesmentioning

confidence: 99%

Human neurotropic polyomavirus, JCV, and its role in carcinogenesis

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Particular isolates of JCV, administered by various routes into hamsters and monkeys, induced the formation of neuroectodermal tumors [56][57][58][59][60][61]. A viral preparation from what was described as a Mad-1-induced owl monkey astrocytoma was injected into a second owl monkey (OWL586).…”

Section: Implications Of Oncogenicitymentioning

confidence: 99%

Viral variant nucleotide sequences help expose leukocytic positioning in the JC virus pathway to the CNS

Jensen

Major

1999

Journal of Leukocyte Biology

View full text Add to dashboard Cite

“…HJC-15 cells (London et al, 1978) were maintained in Dulbecco's modi®ed Eagle's medium supplemented with 10% fetal calf serum (FCS), 100 units/ml of penicillin and 100 mg/ml of streptomycin. The plasmid pTetTAk was transfected into 10 cm plates of HJL-15 cells via calcium phosphate precipitation (Graham and van der Eb, 1973).…”

Section: Plasmids and Cell Linesmentioning

confidence: 99%

Evaluation of an autoregulatory tetracycline regulated system

Gallia

Khalili

1998

Oncogene

View full text Add to dashboard Cite

Tetracycline controlled gene expression systems have become powerful tools in the analysis of gene function in mammalian cell culture as well as transgenic animals and plants. The original description of a tetracyclineregulated gene expression system is based on two plasmids, one of which constitutively expresses a tetracycline-controlled transactivator protein (tTA), a fusion protein between the tetracycline repressor of E. coli and the transcriptional activation domain of the VP16 protein of herpes simplex virus. The second plasmid contains the gene to be regulated by tTA under the control of an inducible promoter which consists of seven copies of the tetracycline resistance operator (tetO). Since this original description, many modi®ca-tions have been described. In this report, we evaluate an autoregulatory tetracycline controlled system, in which the tTA is itself under the control of the tetO. We demonstrate that this autoregulatory tetracycline system produces adverse e ects including cellular morphologic changes, growth rate attenuation and alterations in cell cycle distribution.

show abstract

Brain Tumors in Owl Monkeys Inoculated with a Human Polyomavirus (JC Virus)

Cited by 203 publications

References 14 publications

Human neurotropic polyomavirus, JCV, and its role in carcinogenesis

Human neurotropic polyomavirus, JCV, and its role in carcinogenesis

Viral variant nucleotide sequences help expose leukocytic positioning in the JC virus pathway to the CNS

Evaluation of an autoregulatory tetracycline regulated system

Contact Info

Product

Resources

About