Brain white matter morphological structure correlation with its optical properties estimated from optical coherence tomography (OCT) data

Moiseev, Alexander A.; Achkasova, Ksenia; Kiseleva, Elena B.; Yashin, Konstantin; Potapov, Arseniy L.; Bederina, Evgeniya; Kuznetsov, S. А.; Sherstnev, Evgeny; Шабанов, Д. А.; Gelikonov, Grigory V.; Ostrovskaya, Yuliya; Gladkova, Natalia D.

doi:10.1364/boe.457467

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…CP OCT technology detects polarization-dependent changes and, therefore, represents a promising tool for the assessment of the state of connective tissue in breast cancer and its differentiation from areas of tumor cell clusters based on the registration of cross-polarization backscattering of the OCT signal [29,38]. In addition, in this study, a more recent method of measuring the attenuation coefficient, known as the depth-resolved approach [42,43,46], was used, while in other studies on the evaluation of attenuation coefficient in breast tissue linear fitting method was used [30]. The advantages of the depth-resolved approach involve avoiding axial resolution deterioration from the fitting range and inaccurate attenuation coefficient measurements due to a poor choice of fitting range.…”

Section: Discussionmentioning

confidence: 98%

“…A number of studies have shown that CP OCT is a promising method for differentiating tumorous from non-tumorous breast tissue [29,38] and human brain tissue [39], as well as for the diagnosis of bladder cancer [40]. CP OCT can also measure the attenuation coefficient, which can be helpful for improving and increasing the contrast between tumorous and non-tumorous breast tissues [41][42][43]. Various morphological features of breast cancer are anticipated to influence the polarization qualities of tumor tissue differently.…”

Section: Introductionmentioning

confidence: 99%

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Intraoperative Assessment of Breast Cancer Tissues after Breast-Conserving Surgery Based on Mapping the Attenuation Coefficients in 3D Cross-Polarization Optical Coherence Tomography

Gubarkova

Kiseleva

Moiseev

et al. 2023

Cancers

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Intraoperative differentiation of tumorous from non-tumorous tissue can help in the assessment of resection margins in breast cancer and its response to therapy and, potentially, reduce the incidence of tumor recurrence. In this study, the calculation of the attenuation coefficient and its color-coded 2D distribution was performed for different breast cancer subtypes using spectral-domain CP OCT. A total of 68 freshly excised human breast specimens containing tumorous and surrounding non-tumorous tissues after BCS was studied. Immediately after obtaining structural 3D CP OCT images, en face color-coded attenuation coefficient maps were built in co-(Att(co)) and cross-(Att(cross)) polarization channels using a depth-resolved approach to calculating the values in each A-scan. We determined spatially localized signal attenuation in both channels and reported ranges of attenuation coefficients to five selected breast tissue regions (adipose tissue, non-tumorous fibrous connective tissue, hyalinized tumor stroma, low-density tumor cells in the fibrotic tumor stroma and high-density clusters of tumor cells). The Att(cross) coefficient exhibited a stronger gain contrast of studied tissues compared to the Att(co) coefficient (i.e., conventional attenuation coefficient) and, therefore, allowed improved differentiation of all breast tissue types. It has been shown that color-coded attenuation coefficient maps may be used to detect inter- and intra-tumor heterogeneity of various breast cancer subtypes as well as to assess the effectiveness of therapy. For the first time, the optimal threshold values of the attenuation coefficients to differentiate tumorous from non-tumorous breast tissues were determined. Diagnostic testing values for Att(cross) coefficient were higher for differentiation of tumor cell areas and tumor stroma from non-tumorous fibrous connective tissue: diagnostic accuracy was 91–99%, sensitivity—96–98%, and specificity—87–99%. Att(co) coefficient is more suitable for the differentiation of tumor cell areas from adipose tissue: diagnostic accuracy was 83%, sensitivity—84%, and specificity—84%. Therefore, the present study provides a new diagnostic approach to the differentiation of breast cancer tissue types based on the assessment of the attenuation coefficient from real-time CP OCT data and has the potential to be used for further rapid and accurate intraoperative assessment of the resection margins during BCS.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Intraoperative Assessment of Breast Cancer Tissues after Breast-Conserving Surgery Based on Mapping the Attenuation Coefficients in 3D Cross-Polarization Optical Coherence Tomography

Gubarkova

Kiseleva

Moiseev

et al. 2023

Cancers

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“…It should be noted that areas of damaged white matter are structurally heterogeneous because the tumor has a complex effect on the tissue of the pathways (4). On the one hand, there is a destruction of myelinated nerve fibers, the exact mechanism of which has not yet been established (30). In our previous work (31), we carried out a quantitative assessment of the relationship between the morphological and optical properties of normal white matter, and it was shown that myelinated fibers make the main contribution to its scattering properties.…”

Section: Att(co)mentioning

confidence: 99%

Nondestructive label-free detection of peritumoral white matter damage using cross-polarization optical coherence tomography

et al. 2023

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IntroductionTo improve the quality of brain tumor resections, it is important to differentiate zones with myelinated fibers destruction from tumor tissue and normal white matter. Optical coherence tomography (OCT) is a promising tool for brain tissue visualization and in the present study, we demonstrate the ability of cross-polarization (CP) OCT to detect damaged white matter and differentiate it from normal and tumor tissues.Materials and methodsThe study was performed on 215 samples of brain tissue obtained from 57 patients with brain tumors. The analysis of the obtained OCT data included three stages: 1) visual analysis of structural OCT images; 2) quantitative assessment based on attenuation coefficients estimation in co- and cross-polarizations; 3) building of color-coded maps with subsequent visual analysis. The defining characteristics of structural CP OCT images and color-coded maps were determined for each studied tissue type, and then two classification tests were passed by 8 blinded respondents after a training.ResultsVisual assessment of structural CP OCT images allows detecting white matter areas with damaged myelinated fibers and differentiate them from normal white matter and tumor tissue. Attenuation coefficients also allow distinguishing all studied brain tissue types, while it was found that damage to myelinated fibers leads to a statistically significant decrease in the values of attenuation coefficients compared to normal white matter. Nevertheless, the use of color-coded optical maps looks more promising as it combines the objectivity of optical coefficient and clarity of the visual assessment, which leads to the increase of the diagnostic accuracy of the method compared to visual analysis of structural OCT images.ConclusionsAlteration of myelinated fibers causes changes in the scattering properties of the white matter, which gets reflected in the nature of the received CP OCT signal. Visual assessment of structural CP OCT images and color-coded maps allows differentiating studied tissue types from each other, while usage of color-coded maps demonstrates higher diagnostic accuracy values in comparison with structural images (F-score = 0.85-0.86 and 0.81, respectively). Thus, the results of the study confirm the potential of using OCT as a neuronavigation tool during resections of brain tumors.

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“…The CP OCT method is sensitive to changes of light polarization in tissues. Changes of polarization plane in biological tissues may be caused by double refraction or by microscopic scattering anisotropy [ 13 , 14 ]. This allows to detect anisotropic tissue structures which longitudinally exceed transverse dimensions significantly [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Cross-Polarization Optical Coherence Tomography for Clinical Evaluation of Dermal Lesion Degrees in Vulvar Lichen Sclerosus

Potapov¹,

Loginova²,

Moiseev³

et al. 2023

Sovrem Tehnol Med

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The aim of the study was to identify different degrees of dermal lesions in vulvar lichen sclerosus (VLS) using cross-polarization optical coherence tomography (CP OCT) based on attenuation coefficient to detect disease early manifestations and to monitor the effectiveness of treatment.Materials and Methods. The study included 10 patients without pathology and 39 patients with VLS diagnosed histologically. CP OCT was performed in vivo on the inner surface of the labia minora, in the main lesion area. From each scanning point, a 3.4×3.4×1.25-mm 3 3D data array was obtained in 26 s. CP OCT examination results were compared with histological examination of specimens stained with Van Gieson's picrofuchsin.Quantitative analysis of OCT images was performed by measuring the attenuation coefficient in co-polarization and cross-polarization. For visual analysis, color-coded charts were developed based on OCT attenuation coefficients.Results. According to histological examination, all patients with VLS were divided into 4 groups as per dermal lesion degree: initial (8 patients); mild (7 patients); moderate (9 patients); severe (15 patients). Typical features of different degrees were interfibrillary edema up to 250 μm deep for initial degree, thickened collagen bundles without edema up to 350 μm deep for mild degree, dermis homogenization up to 700 μm deep for moderate degree, dermis homogenization and total edema up to 1200 μm deep for severe degree.Pathological processes in dermis during VLS like interfibrillary edema and collagen bundles homogenization were visualized using CP OCT method based on values of attenuation coefficient in co-and cross-polarization channels. However, CP OCT method appeared to be less sensitive to changes of collagen bundles thickness not allowing to distinguish thickened collagen bundles from normal ones with enough statistical significance. The CP OCT method was able to differentiate all degrees of dermal lesions among themselves. OCT attenuation coefficients differed from normal condition with statistical significance for all degrees of lesions, except for mild. Conclusion.For the first time, quantitative parameters for each degrees of dermis lesion in VLS, including initial degree, were determined by CP OCT method allowing to detect the disease at an early stage and to monitor the applied clinical treatment effectiveness.

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Brain white matter morphological structure correlation with its optical properties estimated from optical coherence tomography (OCT) data

Cited by 10 publications

References 29 publications

Intraoperative Assessment of Breast Cancer Tissues after Breast-Conserving Surgery Based on Mapping the Attenuation Coefficients in 3D Cross-Polarization Optical Coherence Tomography

Intraoperative Assessment of Breast Cancer Tissues after Breast-Conserving Surgery Based on Mapping the Attenuation Coefficients in 3D Cross-Polarization Optical Coherence Tomography

Nondestructive label-free detection of peritumoral white matter damage using cross-polarization optical coherence tomography

Cross-Polarization Optical Coherence Tomography for Clinical Evaluation of Dermal Lesion Degrees in Vulvar Lichen Sclerosus

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