Brainstem Gliomas

Ramos, Ana; Hilario, Amaya; Lagares, Alfonso; Álvarez, Elena Salvador; Pérez-Núñez, Ángel; Sepúlveda, Juan Manuel

doi:10.1053/j.sult.2013.01.001

Cited by 35 publications

(38 citation statements)

References 47 publications

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“…MRI is commonly used to observe neuronal tissue, although still not as familiar as µCT in the laboratory. MRI could visualize internal structures of larger specimens, such as the human 25–27 , cat 28 or mouse brainstem 29 . Our CoMBI system was developed utilizing block-face imaging to avoid deformation artifacts, which are seen in other optical based methods, so that it obtains the intact 3D anatomical data of the specimens.…”

Section: Discussionmentioning

confidence: 99%

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Tajika

Murakami

Iijima

et al. 2017

Sci Rep

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We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.

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Section: Discussionmentioning

confidence: 99%

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Tajika

Murakami

Iijima

et al. 2017

Sci Rep

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“…Paediatric brainstem tumours diffusely infiltrative of the pons—diffuse intrinsic pontine gliomas (DIPG) are the most common type of brainstem glioma, accounting for 60–80 % of these tumours [19]. They are currently the number one cause of brain tumour-related death in children with a median survival of approximately 10 months and <10 % 2-year survival [4, 13].…”

Section: Introductionmentioning

confidence: 99%

Histopathological spectrum of paediatric diffuse intrinsic pontine glioma: diagnostic and therapeutic implications

et al. 2014

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Diffuse intrinsic pontine glioma (DIPG) is the main cause of brain tumour-related death in children. In the majority of cases diagnosis is based on clinical and MRI findings, resulting in the scarcity of pre-treatment specimens available to study. Our group has developed an autopsy-based protocol to investigate the histologic and biologic spectrum of DIPG. This has also allowed us to investigate the terminal pattern of disease and gain a better understanding of what challenges we are facing in treating DIPG. Here, we review 72 DIPG cases with well documented clinical history and molecular data and describe the pathological features of this disease in relation to clinical and genetic features. Fifty-three of the samples were autopsy material (7 pre-treatment) and 19 were pre-treatment biopsy/surgical specimens. Upon histological review, 62 patients had high-grade astrocytomas (18 WHO grade III and 44 WHO grade IV patients), 8 had WHO grade II astrocytomas, and 2 had features of primitive neuroectodermal tumour (PNET). K27M-H3 mutations were exclusively found in tumours with WHO grade II–IV astrocytoma histology. K27M-H3.1 and ACVR1 mutations as well as ALT phenotype were only found in WHO grade III–IV astrocytomas, while PIK3CA mutations and PDGFRA gains/amplifications were found in WHO grade II–IV astrocytomas. Approximately 1/3 of DIPG patients had leptomeningeal spread of their tumour. Further, diffuse invasion of the brainstem, spinal cord and thalamus was common with some cases showing spread as distant as the frontal lobes. These findings suggest that focal radiation may be inadequate for some of these patients. Importantly, we show that clinically classic DIPGs represent a diverse histologic spectrum, including multiple cases which would fit WHO criteria of grade II astrocytoma which nevertheless behave clinically as high-grade astrocytomas and harbour the histone K27M-H3.3 mutation. This suggests that the current WHO astrocytoma grading scheme may not appropriately predict outcome for paediatric brainstem gliomas.Electronic supplementary materialThe online version of this article (doi:10.1007/s00401-014-1319-6) contains supplementary material, which is available to authorized users.

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“…They have a long duration of symptoms before diagnosis, growth patterns of low-grade glial tumors, and follow an indolent course [7,8]. LGBG are focal or grow dorsally exophytic, but in contrast to DIPG do not infiltrate the brainstem white matter tracts [9].…”

Section: Introductionmentioning

confidence: 99%

Diffusion tensor imaging suggests extrapontine extension of pediatric diffuse intrinsic pontine gliomas

Wagner

Bell

Kern

et al. 2016

European Journal of Radiology

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Purpose To apply DTI to detect early extrapontine extension of pediatric diffuse intrinsic pontine glioma along the corticospinal tracts. Methods In children with diffuse intrinsic pontine glioma, low-grade brainstem glioma, and age-matched controls, DTI metrics were measured in the posterior limb of the internal capsule and posterior centrum semiovale. Histological examination was available in one patient. Results 6 diffuse intrinsic pontine glioma, 8 low-grade brainstem glioma, and two groups of 25 controls were included. In diffuse intrinsic pontine glioma compared to controls, fractional anisotropy was lower in the bilateral posterior limb of the internal capsule, axial diffusivity was lower in the bilateral posterior centrum semiovale and posterior limb of the internal capsule, while radial diffusivity was higher in the bilateral posterior limb of the internal capsule. No significant differences were found between low-grade brainstem glioma and controls. In diffuse intrinsic pontine glioma compared to low-grade brainstem glioma, axial diffusivity was lower in the bilateral posterior limb of the internal capsule. Histological examination in one child showed tumor cells in the posterior limb of the internal capsule. Conclusion Reduction in fractional anisotropy and axial diffusivity and increase in radial diffusivity in diffuse intrinsic pontine glioma may reflect tumor extension along the corticospinal tracts as shown by histology. DTI may detect early extrapontine tumor extension in diffuse intrinsic pontine glioma before it becomes apparent on conventional MRI sequences.

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Brainstem Gliomas

Cited by 35 publications

References 47 publications

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Histopathological spectrum of paediatric diffuse intrinsic pontine glioma: diagnostic and therapeutic implications

Diffusion tensor imaging suggests extrapontine extension of pediatric diffuse intrinsic pontine gliomas

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