Branched arabino-oligosaccharides isolated from sugar beet arabinan

Westphal, Yvonne; Kühnel, Stefan; Waard, Pieter de; Hinz, S.W.A.; Schols, Henk A.; Voragen, A.G.J.; Gruppen, Harry

doi:10.1016/j.carres.2010.03.042

Cited by 101 publications

(48 citation statements)

References 15 publications

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“…The isolated arabino-oligosaccharides represent many different substitution patterns of the arabinan backbone. These linear and branched arabino-oligosaccharides (Figure 1) with the exceptions of A-II2 and A-IV2 were previously isolated and characterized by Westphal et al 20 The NMR spectroscopic data of the isolated oligosaccharides (Supporting Information) were in good agreement with data published by Westphal et al 20 The novel arabinan tetramer branched at position O-2 (A-IV2) was liberated in very low quantities from sugar beet arabinans and was also difficult to separate from the O-3 branched tetramer by RP18-HPLC. Thus, this tetramer was isolated from red clover sprout polysaccharides, where Compound A-II2, which was isolated from an endoarabinanase hydrolysate of amaranth insoluble fiber, contains an α-L-arabinopyranose attached to O-5 of an arabinofuranose.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 94%

Neutral Pectin Side Chains of Amaranth (Amaranthus hypochondriacus) Contain Long, Partially Branched Arabinans and Short Galactans, Both with Terminal Arabinopyranoses

Wefers

Tyl

Bunzel

2015

J. Agric. Food Chem.

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Amaranth is a pseudocereal of high nutritional value, including a high dietary fiber content. Amaranth dietary fiber was suggested to contain large amounts of neutral rhamnogalacturonan I side chains. In this study, endo-arabinanase and endo-galactanase were used to liberate arabinan and galactan oligosaccharides from amaranth fiber. The liberated oligosaccharides were identified by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and HPLC-MS(n) using standard compounds, which were isolated from amaranth, sugar beet, potato, and red clover sprouts and characterized by one- and two-dimensional NMR spectroscopy. It was demonstrated that insoluble amaranth arabinans have linear and branched areas, with the O-3 position being the dominant branching point. Minor amounts of branches at position O-2 and double substitution were also found. Amaranth arabinans were also demonstrated to contain terminal α-(1→5)-linked l-arabinopyranose units. In addition, it was evidenced that galactans from amaranth seeds are composed of β-(1→4)-linked d-galactopyranose units, which can also be terminated with l-arabinopyranose units. In direct comparison to structural elucidation of amaranth fiber by using methylation analysis, the advantage of the enzymatic approach over methylation analysis was demonstrated.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 94%

Neutral Pectin Side Chains of Amaranth (Amaranthus hypochondriacus) Contain Long, Partially Branched Arabinans and Short Galactans, Both with Terminal Arabinopyranoses

Wefers

Tyl

Bunzel

2015

J. Agric. Food Chem.

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“…ECP fimbriae bound preferentially to pectin fractions in polysaccharides extracted from spinach compared with long oligomers of arabinan purified from beet roots that were either branched or unbranched (63). Sugar beet arabinan consists of an ␣-(135)-linked backbone of L-arabinosyl residues that are either single or double substituted (64). Treatment of unbranched arabinan to remove (132)-and (133)-␣-L-arabinofuranosyl branches revealed a specificity of ECP Sakai , indicating a potential affinity for ␣-(135)-linked L-arabinosyl residues and longer chains of arabinan.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli Common Pilus (ECP) Targets Arabinosyl Residues in Plant Cell Walls to Mediate Adhesion to Fresh Produce Plants

Rossez

Holmes

Lodberg-Pedersen

et al. 2014

Journal of Biological Chemistry

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Background: Bacterial fimbriae mediate binding to host tissue through specific interactions. Results: ECP interacts with arabinosyl residues in pectin and other plant cell wall components. Conclusion: ECP-arabinan interactions facilitate binding of E. coli to plant hosts. Significance: The prevalence of arabinan targets in produce plants together with ECP expression may explain the association of pathogenic bacteria in edible plants.

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“…They are generally considered to be associated with RG-I in the pectic network with a backbone of (1,5)-linked a-Larabinofuranosyl units (Gomez et al, 2009;Westphal et al, 2010). As shown in Table 6, arabinans branch mostly on O-3, or both O-2 and O-3, while O-2 branching can be found in mung bean (Swamy & Salimath, 1991), mustard seed (Tharanathan, Bhat, Krishna, & Paramahans, 1985), dehulled rapeseed (Eriksson, Andersson, Westerlund, Andersson, & Aman, 1996), honey locust seed (Navarro et al, 2002), horsebean root (Joseleau, Chambat, & Lanvers, 1983), and prickly pear (Habibi et al, 2005).…”

Section: Discussionmentioning

confidence: 99%