Branchial and intestinal osmoregulatory acclimation in the four-eyed sleeper, Bostrychus sinensis (Lacepède), exposed to seawater

Peh, Wendy Yen Xian; Chew, Shit F.; Wilson, Jonathan M.; Ip, Yuen K.

doi:10.1007/s00227-009-1210-5

Cited by 15 publications

(10 citation statements)

References 37 publications

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“…In the Japanese medaka, intestinal NKA activity increased with environmental salinity. Similar results were found in the other teleosts [ 16 , 38 – 40 ]. Meanwhile, in certain teleosts including the California Mozambique tilapia ( Oreochromis mossambicus x O .…”

Section: Discussionsupporting

confidence: 91%

Intestinal FXYD12 and sodium-potassium ATPase: A comparative study on two euryhaline medakas in response to salinity changes

et al. 2018

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FXYD proteins are the regulators of sodium-potassium ATPase (Na+/K+-ATPase, NKA). In teleosts, NKA is a primary driving force for the operation of many ion transport systems in the osmoregulatory organs (e.g. intestines). Hence, the purpose of this study was to determine the expression of FXYD proteins and NKA α-subunit in the intestines of two closely related medakas (Oryzias dancena and O. latipes), which came from different salinity habitats and have diverse osmoregulatory capabilities, to illustrate the association between NKA and FXYD proteins of two medaka species in response to salinity changes. The results showed that the fxyd12 mRNA was the most predominant in the intestines of both medakas. The association of FXYD12 and NKA in the intestines of the two medaka species was demonstrated via double immunofluorescent staining and co-immunoprecipitation. Upon salinity challenge, the localization of FXYD12 and NKA was similar in the intestines of the two medaka species. However, the expression profiles of intestinal FXYD12 and NKA (mRNA and protein levels), as well as NKA activity differed between the medakas. These results showed that FXYD12 may play a role in modulating NKA activity in the intestines of the two medakas following salinity changes in the maintenance of internal homeostasis. These findings contributed to knowledge of the expression and potential role of vertebrate FXYD12, the regulators of NKA, upon salinity challenge.

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Section: Discussionsupporting

confidence: 91%

Intestinal FXYD12 and sodium-potassium ATPase: A comparative study on two euryhaline medakas in response to salinity changes

et al. 2018

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“…during seawater acclimation and the excretion of NH þ 4 against an NH þ 4 concentration during environmental ammonia exposure involved similar transporters (i.e., Cftr, Nkcc1 and Nka) but different types of MRCs in the gills of A. testudineus. Although some euryhaline freshwater ) or brackish water (Peh et al 2009) fishes may have the ability to upregulate these branchial transport mechanisms during seawater acclimation, it becomes apparent from this study that only those capable of upregulating these branchial transport mechanisms in response to environmental ammonia can actively excrete ammonia. More importantly, unlike seawater acclimation whereby Na ?…”

Section: Resultsmentioning

confidence: 96%

Cystic fibrosis transmembrane conductance regulator in the gills of the climbing perch, Anabas testudineus, is involved in both hypoosmotic regulation during seawater acclimation and active ammonia excretion during ammonia exposure

Wilson²,

Loong

et al. 2012

J Comp Physiol B

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This study aimed to clone and sequence the cystic fibrosis transmembrane conductance regulator (cftr) from, and to determine the effects of seawater acclimation or exposure to 100 mmol l⁻¹ NH₄Cl in freshwater on its mRNA and protein expressions in, the gills of Anabas testudineus. There were 4,530 bp coding for 1,510 amino acids in the cftr cDNA sequence from A. testudineus. The branchial mRNA expression of cftr in fish kept in freshwater was low (<50 copies of transcript per ng cDNA), but significant increases were observed in fish acclimated to seawater for 1 day (92-fold) or 6 days (219-fold). Branchial Cftr expression was detected in fish acclimated to seawater but not in the freshwater control, indicating that Cl⁻ excretion through the apical Cftr of the branchial epithelium was essential to seawater acclimation. More importantly, fish exposed to ammonia also exhibited a significant increase (12-fold) in branchial mRNA expression of cftr, with Cftr being expressed in a type of Na⁺/K⁺-ATPase-immunoreactive cells that was apparently different from the type involved in seawater acclimation. It is probable that Cl⁻ excretion through Cftr generated a favorable electrical potential across the apical membrane to drive the excretion of NH₄⁺ against a concentration gradient through a yet to be determined transporter, but it led to a slight loss of endogenous Cl⁻. Since ammonia exposure also resulted in significant decreases in blood pH, [HCO₃⁻] and [total CO₂] in A. testudineus, it can be deduced that active NH₄⁺ excretion could also be driven by the exit of HCO₃⁻ through the apical Cftr. Furthermore, A. testudineus uniquely responded to ammonia exposure by increasing the ambient pH and decreasing the branchial bafilomycin-sensitive V-type H⁺-ATPase activity, which suggests that its gills might have low NH₃ permeability.

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“…This branchial importance in ion regulation seems to have been kept in air‐breathing fish in spite of their pronounced reduction in gill surface area (e.g. Brauner, Matey, Wilson, Bernier & Val ; Chang, Loong, Wong, Chew, Wilson & Ip ; Peh, Chew, Wilson & Ip ).…”

Section: Introductionmentioning

confidence: 99%

Effects of salinity on osmoregulation, growth and survival in Asian swamp eel (Monopterus albus)(Zuiew 1793)

et al. 2012

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The Asian swamp eel Monopterus albus is normally considered a freshwater species, but can also occasionally be found in brackish water. It is an obligate air-breather with highly reduced gills, making its osmoregulation physiology interesting because the gills normally represent the primary site of osmoregulation in teleosts. Being a popular fish for human consumption, the swamp eel is cultured extensively in freshwater ponds in the Mekong Delta of Vietnam. However, very little is known about its salinity tolerance, which is becoming topical due to the increasing salt-water intrusions into tropical deltas. We therefore studied how increased salinity affects survival, growth, blood pressure, heart rate, blood osmolality and plasma ions. All eels survived prolonged exposure to 5 and 10 g L À1 , although plasma osmolality increased significantly at 10 g L À1 . Further elevation to 15 and 20 g L À1 was associated with significantly elevated mortality, with a corresponding increase in plasma osmolality and ion concentrations. Our results show that swamp eels thrive in 0-10 g L À1 with an optimum growth between 0 and 9 g L À1 , indicating that utilization of low saline brackish waters for aquaculture is possible.

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Branchial and intestinal osmoregulatory acclimation in the four-eyed sleeper, Bostrychus sinensis (Lacepède), exposed to seawater

Cited by 15 publications

References 37 publications

Intestinal FXYD12 and sodium-potassium ATPase: A comparative study on two euryhaline medakas in response to salinity changes

Intestinal FXYD12 and sodium-potassium ATPase: A comparative study on two euryhaline medakas in response to salinity changes

Cystic fibrosis transmembrane conductance regulator in the gills of the climbing perch, Anabas testudineus, is involved in both hypoosmotic regulation during seawater acclimation and active ammonia excretion during ammonia exposure

Effects of salinity on osmoregulation, growth and survival in Asian swamp eel (Monopterus albus)(Zuiew 1793)

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