Brassica Improvement Through Microspore Culture

Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project "Czech Winter Rape"

“…In the Czech Republic, the cultivation and breeding of oilseed rapes have become increasingly dominated by hybrid varieties based on seed production from inbreds with cytoplasmic male sterility as a quality donor and a fertility restorer as male line providing pollen. Microspore culture can aid in the selection of restorer lines, and thus increase the efficiency of this programme (WANG et al 1999in FERRIE & KELLER 2004. A biotechnological approach to the production of doubled haploid plants via culture of microspores may be successfully applied to affect a number of morphological /agronomic/ qualitative traits contributing both to high yield and desirable seed composition.…”

“…ADAMSKA et al (2004) produced DH lines with a modified fatty acid composition with heritable and environmental stability. According to FERRIE and KELLER (2004) there are currently more than fifty oilseed Brassica varieties in commercial production derived by a doubled haploid technique.…”

“…Brassica napus L. is an allotetraploid or amphidiploid species with n = 19 chromosomes, derived from 2 diploid species B. oleracea (n = 9) and B. rapa (n = 10). The microspore doubled haploid (DH) methodology is now employed in many B. napus breeding programmes around the world as an alternative/supplement to conventional methods of homozygous line production (KUČERA et al 2002FERRIE & KELLER 2004;FRIEDT & ZARHLOUL 2005;KOPRNA et al 2005 Abstract: A selected set of twenty inter-line F 1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project "Czech Winter Rape". All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes.…”

“…Brassica napus L. is an allotetraploid or amphidiploid species with n = 19 chromosomes, derived from 2 diploid species B. oleracea (n = 9) and B. rapa (n = 10). The microspore doubled haploid (DH) methodology is now employed in many B. napus breeding programmes around the world as an alternative/supplement to conventional methods of homozygous line production (KUČERA et al 2002FERRIE & KELLER 2004;FRIEDT & ZARHLOUL 2005;KOPRNA et al 2005). The doubled haploid method may reduce the time …”

Improvement of microspore culture method for multiple samples in Brassica

“…In genus Brassica, since successful isolated microspore culture of B. napus was reported by Lichter (1982), a large amount of research has been carried out to improve this technique and to expand it other Brassica species and allied genera (Ferrie and Keller 2004, Palmer and Keller 1999, Takahata 1997. Microspore culture of Brassicas has several advantages; 1) the techniques of microspore culture are simple and easier than those of anther culture, 2) a high yield of embryos can be obtained within a short period of 2-3 weeks, and 3) this technique offers not only the fastest route to homozygosity, but it also provides several valuable tools for genetic engineering and a model system of developmental research because of its single-cell system (Takahata et al 2005).…”