Brassica yellows virus’ movement protein upregulates anthocyanin accumulation, leading to the development of purple leaf symptoms on Arabidopsis thaliana

Abstract

Poleroviruses are widely distributed and often of great economic importance because they cause a variety of symptoms, such as the rolling of young leaves, leaf color changes, and plant decline, in infected plants. However, the molecular mechanism behind these viral-induced symptoms is still unknown. Here, we verified the pathogenicity of the polerovirus Brassica yellows virus (BrYV) by transforming its full-length amplicon into Arabidopsis thaliana, which resulted in many abnormal phenotypes. To better understand the interactions between BrYV and its host, global transcriptome profiles of the transgenic plants were compared with that of non-transgenic Arabidopsis plants. An association between the BrYV- induced purple leaf symptoms and the activation of anthocyanin biosynthesis was noted. Using the transgenic approach, we found that movement protein of BrYV was responsible for the induction of these coloration symptoms. Collectively, our findings demonstrate the BrYV’ pathogenicity and show that the BrYV-induced purple leaf symptom resulted from its movement protein stimulating anthocyanin accumulation.

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

“…Ustilago maydis triggered anthocyanin induction in maize 80 , and anthocyanin-enriched tomato fruits exhibited lower susceptibility to gray mold 81 . Moreover, antibacterial, antifungal and antiviral activities of certain anthocyanins were also reported [82][83][84] .…”

Development of polyclonal antisera against movement proteins of the three poleroviruses infecting cucurbits and their serological relationships

“…Therefore, it is important to study how MP participates in the movement of the virus in hosts. MP plays an essential role in virus infection, which not only promotes the intercellular movement and systemic movement of the virus but also serves as a critical pathogenic factor to cause purple symptoms in the infected leaves [44]. Thus, we establish a specific detection system by developed antisera against three different MPs, which is indispensable to study the occurrence and distribution of viruses and the function of MP.…”

A Simple Method for Acquisition and Inoculation of Brassica Yellows Virus from Transgenic Plants and Frozen Infected Leaves by Aphids

“…There are two BrYV inocula were selected for aphids feeding and transmission. Firstly, the seedlings of the seventh generation transgenic A. thaliana line 412 with BrYV full-length cDNA clone grown to 3-4 weeks with all shown typical purple symptoms and were confirmed to carry BrYV by western blotting and RT-PCR which were used as fresh BrYV inocula [14]. And the transgenic plants were also kept at -20 ℃ as frozen BrYV inocula.…”

“…genotypes (BrYV-A, B, and C) according to sequence analysis, the full-length infectious cDNA clones of these three genotypes have been developed successfully [8,[10][11][12][13]. Previously work have shown that the full-length amplicon of the virus has been successfully transferred into Arabidopsis thaliana and obtained stable genetically transgenic lines named 111 and 412 which all exhibited severe symptoms including dwarfing and the purple leaves [14]. BrYV P0 protein interacted with the plant SKP1 contributes to its stability thereby evading autophagy and proteasomal degradation [15].…”

“…Up to now, there are no reference that aphids acquire BrYV from transgenic plants with virus full length genome-germinated from seeds kept in storage and frozen infected leaves as a virus inoculation material. As BrYV infectious cDNA clone could infected model plants like Arabidopsis thaliana and Nicotiana benthamiana mediated by Agrobacterium infiltration [13,14]. For the reason of Agrobacterium infiltration method cannot completely replace natural virus inoculation conditions.…”