Brassinosteroid gene regulatory networks at cellular resolution in the
            <i>Arabidopsis</i>
            root

Nolan, Trevor M.; Vukašinović, Nemanja; Hsu, CY; Zhang, Jingyuan; Vanhoutte, Isabelle; Shahan, Rachel; Taylor, Isaiah; Greenstreet, Laura; Heitz, Matthieu; Afanassiev, Anton; Wang, Ping; Szekely, Pablo; Brosnan, Aiden; Yin, Yanhai; Schiebinger, Geoffrey; Ohler, Uwe; Russinova, Eugenia; Benfey, Philip N.

doi:10.1126/science.adf4721

Cited by 56 publications

(41 citation statements)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, BL treatment has been shown to alter the expression profiles of various genes in roots (28). To explore whether the gene expression profile in pollen tubes was affected by BL treatment, we performed RNA-seq analysis.…”

Section: Resultsmentioning

confidence: 99%

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Matsuura-Tokita,

Suzuki,

Kimata

et al. 2024

Preprint

View full text Add to dashboard Cite

Brassinosteroids (BRs) are steroid hormones identified in plants. Besides promoting cell elongation and division, BRs facilitate the development of both male and female reproductive tissues. In animals, reproductive steroid hormones play an essential role in reproductive tissue development by regulating gene expression. Here, we focused on the function of BRs during fertilization. We measured the content of biologically active BRs, brassinolide (BL) and castasterone (CS), in the reproductive tissues of Arabidopsis thaliana. Both BL and CS accumulated abundantly in pollen grains and in larger amounts in pistils than in leaves. To evaluate BL function during fertilization, we used an in vitro guidance assay with exogenously applied BL. Although pollen tubes need to be elongated through the pistils for efficient capacitation, BL treatment promoted pollen tube capacitation and improved attraction to ovules in vitro. Transcriptome analysis demonstrated that BL treatment induced the expression of half of the genes expressed in pollen tubes that elongated through the pistils. These results indicated that BL supplied from pistils is a key factor for pollen tube capacitation. However, using the bri1 mutant for the guidance assay resulted in reduced pollen tube capacitation, suggesting that BRI1-signaling in pistils is also important. Furthermore, BRs act on ovules. Exogenous BL application to ovules maintained guidance capacity by promoting the expression of small secreted proteins involved in pollen tube attraction and gamete fusion. Overall, BRs play a significant role as male and female reproductive hormones throughout the plant fertilization process.

show abstract

Section: Resultsmentioning

confidence: 99%

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Matsuura-Tokita,

Suzuki,

Kimata

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…None of them were previously associated with low temperature (unless HB13 and ERD7) and related to RH growth highlighting the predictive power of this approach. Previously, based on single-cell RNA sequencing, it was identified several TFs similar to our gene regulatory network (GRN) such as HB3 (HAT7) and GTL1 as responsive to brassinosteroids in the cortex during cell elongation (Nolan et al 2023). This suggests that similar GRN might be sub-functionalized in different cellular contexts and under different environmental and hormonal cues.…”

Section: Discussionmentioning

confidence: 96%

Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature

Urzúa Lehuedé,

Berdion Gabarain,

Ibeas

et al. 2024

Preprint

View full text Add to dashboard Cite

The root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous and external signals, which are combined to form an integrative response. Surprisingly, a low temperature condition of 10C causes an increased RH growth in Arabidopsis and in several monocots, even when the development of the rest of the root and aerial parts of the plant are halted. Previously, we demonstrated a strong correlation between the growth response and a significant decrease in nutrient availability in the medium under low temperature conditions. However, the molecular basis responsible for receiving and transmitting signals related to the availability of nutrients in the soil, and their relation to plant development, remain largely unknown. We decided to further investigate the intricate molecular processes behind the particular responsiveness of this root cell type at low temperature. In this study, we have discovered a gene regulatory network (GRN) controlling early transcriptome responses to low temperature. This GNR is commanded by specific transcription factors (FTs), namely ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4), a member of the homeodomain leucine zipper (HD-Zip I) group I 13 (AtHB13), the trihelix TF GT2-LIKE1 (GTL1), and a previously unidentified MYB-like TF (AT2G01060). Furthermore, we have identified four downstream RSL4 targets AtHB16, AtHB23, EARLY-RESPONSIVE TO DEHYDRATION 7 (ERD7) and ERD10 suggesting their participation in the regulation of RH development under these conditions. Functional analysis shows that such components of the RSL4-dependent transcriptional cascade influence the subsequent RH growth response to low temperature. These discoveries enhance our comprehension of how plants synchronize the RH growth in response to variations in temperature and nutrient availability at the cellular level.

show abstract

“…Arabidopsis thaliana accession Col-0 plants expressing a previously described pCASP2:NLS:GFP:GUS reporter for endodermis ( pCASP2:GFP ) 28 , or a pCORTEX:erGFP reporter for cortex ( pCORTEX:GFP) 29 , were used for these experiments. Plant growth and protoplast isolation were performed as described 2 , with minor modifications to accommodate proteomic analysis. Seeds were sterilized using 50% (v/v) bleach with 0.05% Tween-20 for 10-15 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Yet, these bulk analyses merely capture the mean expression within a mix of cells and cell types, thus concealing the intrinsic heterogeneity of expression between individual cells. Advances in single-cell RNA sequencing (scRNA-seq) are unraveling cellular heterogeneity and providing unique insight into biological systems 1,2 . It has long been recognized that genome-wide correlations between the levels of proteins and mRNAs are weakly positive in all organisms studied, including plants [3][4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Single-cell proteomics differentiates Arabidopsis root cell types

Montes,

Zhang,

Nolan

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Recently, major advances have enabled the exploration of cellular heterogeneity using single-cell proteomics. Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell. From 756 cells we identify 3,751 proteins and 1,114 proteins/cell. Ultimately, we focus on 3,101 proteins quantified following stringent filtering. Of these, we identified 555 proteins whose expression is enriched in either the cortex or endodermis and are able to differentiate these closely related plant cell-types. Collectivity, our findings underscore the promise of single-cell proteomics to explore the heterogeneity of expression between individual plant cells.

show abstract

Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

Cited by 56 publications

References 130 publications

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature

Single-cell proteomics differentiates Arabidopsis root cell types

Contact Info

Product

Resources

About