Brassinosteroids Confer Tolerance to Salt Stress in Eucalyptus urophylla Plants Enhancing Homeostasis, Antioxidant Metabolism and Leaf Anatomy

Oliveira, Victor Pereira de; Lima, Michael Douglas Roque; Silva, Breno Ricardo Serrão da; Batista, Bruno Lemos; Lobato, Allan Klynger da Silva

doi:10.1007/s00344-018-9870-3

Cited by 53 publications

(34 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, Kapoor et al (2016) reported that EBL alleviated the harmful impact of Cd in radish seedlings (Raphanus sativus) by enhancing the level of antioxidants, such as ascorbic acid, GSH, and tocopherols. In the case of soybean seedlings (Glycine max) (Ribeiro et al 2019) and cowpea (Vigna unguiculata) (Lima and Lobato 2017) under water deficit conditions and Eucalyptus urophylla exposed to salt stress (Oliveira et al 2019), EBL caused increases in SOD, CAT, APX, and POX activities, indicating that BR has a significant impact on the antioxidant system in plants subjected to abiotic stresses. Additionally, EBL reduced the superoxide and H 2 O 2 levels as well as membrane damages (MDA and electrolyte leakage) in soybean under water deficit (Pereira et al 2019).…”

Section: Discussionmentioning

confidence: 99%

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

Talarek-Karwel

Bajguz

Piotrowska‐Niczyporuk

2019

J Appl Phycol

View full text Add to dashboard Cite

Aquatic organisms are exposed to many stressors, e.g., heavy metals. Brassinosteroids, a plant hormone group, can effectively stimulate plants to defend against the negative impact of a heavy metal. The present study was conducted with an aim to find out the influence of 24-epibrassinolide (EBL) on Acutodesmus obliquus treated with 0.01 and 500 μM of lead (Pb) ions during 7 days of cultivation. Pb has a toxic effect on algal cultures because it limits both the growth and development and induces oxidative stress. Simultaneously, 1 μM EBL was involved in protecting algal cells against the toxic effect of Pb. Despite the presence of Pb, EBL significantly increased the number of algal cells and their metabolite content (e.g., proteins, monosaccharides, chlorophylls, carotenes, and xanthophylls). Cultures treated concurrently with EBL and Pb were characterized by a reduction in their content of endogenous Pb, H 2 O 2 , and malondialdehyde. Also, EBL increased the activity of catalase, ascorbate peroxidase, superoxide dismutase, glutathione reductase, and the content of ascorbate and glutathione. EBL increased the phytochelatin synthase activity, thereby enhancing the production of phytochelatins accountable for both binding and detoxification of Pb. These results indicate the influence of EBL on the inhibitory effect of Pb in A. obliquus. These findings help to clarify the role of BRs in the algal adaptation to the prevailing stressful conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

Talarek-Karwel

Bajguz

Piotrowska‐Niczyporuk

2019

J Appl Phycol

View full text Add to dashboard Cite

show abstract

“…The 0 and 100 nM EBR (Sigma-Aldrich, USA) solutions were prepared in agreement with Ahammed et al (2013). Based on preliminary studies and literature available (Lima and Lobato 2017;Maia et al 2018;Pereira et al 2019;Oliveira et al 2019), the EBR is more efficient in plants pretreated (10th day). On the other hand, Zn was applied only on 20th day after experimental implementation due to need of leaf area and plant tissue sufficient to make all analyses involved in this research.…”

Section: -Epibrassinolide (Ebr) Preparation and Applicationmentioning

confidence: 99%

“…Zn is the second most common micronutrient required by plants; however, deficiencies and excesses of Zn promote deleterious effects on soybean plants. Interestingly, EBR can be a possible solution to mitigate the damage caused by deficiencies and excesses of Zn in plants because this steroid presents a spectrum of actions linked to increments in nutrient content (Lima et al 2018), reactive oxygen species scavenging (Oliveira et al 2019) and stimulation of biomass (Maia et al 2018). Therefore, the aim of this research was to determine whether EBR can mitigate oxidative stress in soybean plants subjected to different Zn supplies and to evaluate its possible repercussions on anatomical, nutritional, biochemical, physiological and morphological behaviours.…”

Section: Introductionmentioning

confidence: 99%

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

Santos

Silva

Pedron

et al. 2019

J Soil Sci Plant Nutr

Self Cite

View full text Add to dashboard Cite

The aim of this research was to determine whether 24-epibrassinolide can mitigate oxidative stress in soybean plants subjected to different zinc levels; to examine this, we evaluated the possible repercussions on anatomical, nutritional, biochemical, physiological and morphological behaviours. The experiment followed a completely randomized factorial design with two concentrations of 24-epibrassinolide (0 and 100 nM EBR, described as-EBR and + EBR, respectively) and three zinc supplies (0.2, 20 and 2000 μM Zn, described as low, control and a high supply of Zn). In general, low and high zinc supplies produced deleterious effects. However, plants exposed to high zinc +100 nM EBR presented increases of 25%, 7%, 9% 29% and 69% for root epidermis, root endodermis, root cortex, vascular cylinder and metaxylem, respectively, when compared to the same treatment without the steroid. The steroid spray alleviated the impact produced by zinc stress on nutritional status, and these results were intrinsically linked to incremental changes in root structure, mainly vascular cylinder and metaxylem. Antioxidant enzymes play crucial roles in the photosynthetic machinery of plants treated with 24-epibrassinolide and stressed by high and low zinc supply, modulating reactive oxygen species scavenging and protecting the chloroplast membranes, with clear positive repercussions on photosystem II efficiency and photosynthetic pigments. The stimulation induced by this steroid on gas exchange can be explained by the favourable conditions detected in stomatal performance and leaf anatomy, thus enhancing the diffusion of carbon dioxide.

show abstract

“…Until now, there are no reports about the relationship between EBR and Fe excess and no one has yet reported any mitigation of iron toxicity by EBR. Our hypothesis is that Fe toxicity causes negative interference on photosynthetic machinery and that one way to counteract the damages is the exogenous application of EBR, since this steroid may exercise multiple roles, contributing to ion homeostasis (Oliveira et al ., 2019), improved anatomical responses (Maia et al ., 2018), stimulation of antioxidant enzymes (Lima and Lobato, 2017), and higher carbon dioxide (CO 2 ) fixation (Lima et al ., 2018). This research focused on answering the question whether EBR can contribute to Fe tolerance in rice plants and evaluate possible changes in anatomical characteristics, nutrient concentrations, antioxidant system, and gas exchange parameters.…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids trigger tolerance to iron toxicity in rice

Tadaiesky

Silva

Batista

et al. 2020

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Iron (Fe) toxicity is one of the most frequent abiotic stresses in rice, as it affects from 15% to 30% of the total production. Brassinosteroids (BRs), including 24‐epibrassinolide (EBR), regulate ion homeostasis and improve the antioxidant system. The aim of this research was to determine whether EBR can contribute to the tolerance of rice plants exposed to Fe toxicity and to evaluate the possible effect on anatomical characteristics, nutrient concentrations, the antioxidant system, and gas exchange. The experiment was randomized with four treatments, two with different concentrations of Fe (250 and 6250 μM, control and toxicity, respectively) and these were either supplied with EBR or not (0 and 10 nM EBR, described as −EBR and +EBR, respectively). Treating plants grown under Fe toxic conditions with EBR caused an 70% increase in root aerenchyma area, compared to plants without steroid treatment. Our results revealed that EBR treatment could mitigate the deleterious effects of Fe toxicity in rice plants, by modulating the aerenchyma area, which contributes to the formation of an oxidative barrier and reduce the Fe mobilization at the root surface. Plants that were exposed to Fe toxic concentrations and treated with EBR showed (1) an increase in the enzyme activities of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase, (2) mitigation of oxidative damage and (3) increased scavenging of reactive oxygen species. Finally, EBR alleviated the negative impacts induced by excess Fe on the net photosynthetic rate and the instantaneous carboxylation efficiency. These benefits were directly related to higher electron transport and stomatal density and indirectly linked to the protection mechanism exercised by the antioxidant enzymes on photosynthetic machinery. We conclude that EBR is able to confer tolerance to Fe toxicity in rice plants.

show abstract

Brassinosteroids Confer Tolerance to Salt Stress in Eucalyptus urophylla Plants Enhancing Homeostasis, Antioxidant Metabolism and Leaf Anatomy

Cited by 53 publications

References 78 publications

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

Brassinosteroids trigger tolerance to iron toxicity in rice

Contact Info

Product

Resources

About