Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function

Díaz-Pascual, Francisco; Hartmann, Raimo; Lempp, Martin; Vidakovic, Lucia; Song, Boya; Jeckel, Hannah; Thormann, Kai M.; Yildiz, Fitnat H.; Dunkel, Jörn; Link, Hannes; Nadell, Carey D.; Drescher, Knut

doi:10.1038/s41564-019-0579-2

Cited by 73 publications

(69 citation statements)

References 47 publications

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“…Genetic segregation of fluorescently labelled strains in experiments suggests a strong reduction in genetic diversity compared to the ancestral population 11 . Limited by the availability of differentiable fluorescent proteins, experimental observations are restricted to a coarse resolution and are not capable of resolving individual lineages, although efforts have been made to include multiple fluorescent proteins 13 , high temporal resolution imaging 36 , and also three dimensional tracking 37 . An individual-based model on the other hand is capable to resolve the segregation dynamics based on individual inoculated cells, and enabled us to quantify the number of individual cell lineages persisting at the expanding colony edge depending on trophic interaction.…”

Section: Discussionmentioning

confidence: 99%

Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages

et al. 2020

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Evidence suggests that bacterial community spatial organization affects their ecological function, yet details of the mechanisms that promote spatial patterns remain difficult to resolve experimentally. In contrast to bacterial communities in liquid cultures, surface-attached range expansion fosters genetic segregation of the growing population with preferential access to nutrients and reduced mechanical restrictions for cells at the expanding periphery. Here we elucidate how localized conditions in cross-feeding bacterial communities shape community spatial organization. We combine experiments with an individual based mathematical model to resolve how trophic dependencies affect localized growth rates and nucleate successful cell lineages. The model tracks individual cell lineages and attributes these with trophic dependencies that promote counterintuitive reproductive advantages and result in lasting influences on the community structure, and potentially, on its functioning. We examine persistence of lucky lineages in structured habitats where expansion is interrupted by physical obstacles to gain insights into patterns in porous domains.

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Section: Discussionmentioning

confidence: 99%

Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages

et al. 2020

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“…Tetracycline also promotes the biofilm formation of the pathogen Acinetobacter baumannii , and whole-genome sequence analysis revealed an increase in the rate of mutations such as SNPs, as well as insertions and deletions, under subinhibitory drug exposition [ 285 ]. In addition to the accumulation of genotypic variation, biofilm treatment with a low level of antibiotics produces changes in the gene expression profile, some of which may be linked to increased biofilm formation [ 283 , 285 , 286 ].…”

Section: Conjugation In Natural Habitats: the Example Of Bacterialmentioning

confidence: 99%

Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level

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Djermoun

et al. 2020

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Bacterial conjugation, also referred to as bacterial sex, is a major horizontal gene transfer mechanism through which DNA is transferred from a donor to a recipient bacterium by direct contact. Conjugation is universally conserved among bacteria and occurs in a wide range of environments (soil, plant surfaces, water, sewage, biofilms, and host-associated bacterial communities). Within these habitats, conjugation drives the rapid evolution and adaptation of bacterial strains by mediating the propagation of various metabolic properties, including symbiotic lifestyle, virulence, biofilm formation, resistance to heavy metals, and, most importantly, resistance to antibiotics. These properties make conjugation a fundamentally important process, and it is thus the focus of extensive study. Here, we review the key steps of plasmid transfer by conjugation in Gram-negative bacteria, by following the life cycle of the F factor during its transfer from the donor to the recipient cell. We also discuss our current knowledge of the extent and impact of conjugation within an environmentally and clinically relevant bacterial habitat, bacterial biofilms.

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“…In a recent study Díaz-Pascual et al (2019) examined the effects of antibiotics commonly used to treat cholera on biofilms of Vibrio cholerae . Transient exposure to translation-inhibiting antibiotics such as tetracycline caused alterations in cell shape and physiology that resulted in large-scale changes in biofilm architecture and the dismantling of cell-matrix associations.…”

Section: Biofilms In Infectionsmentioning

confidence: 99%

Secondary Effects of Antibiotics on Microbial Biofilms

et al. 2020

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Biofilms are assemblages of microorganisms attached to each other, or to a surface, and encased in a protective, self-produced matrix. Such associations are now recognized as the predominant microbial growth mode. The physiology of cells in biofilms differs from that of the planktonic cells on which most research has been conducted. Consequently, there are significant gaps in our knowledge of the biofilm lifestyle. Filling this gap is particularly important, given that biofilm cells may respond differently to antibiotics than do planktonic cells of the same species. Understanding the effects of antibiotics on biofilms is of paramount importance for clinical practice due to the increased levels of antibiotic resistance and resistance dissemination in biofilms. From a wider environmental perspective antibiotic exposure can alter the ecology of biofilms in nature, and hence disrupt ecosystems. Biofilm cells display increased resilience toward antibiotics. This resilience is often explained by mechanisms and traits such as decreased antibiotic penetration, metabolically inactive persister cells, and intrinsic resistance by members of the biofilm community. Together, these factors suggest that cells in biofilms are often exposed to subinhibitory concentrations of antimicrobial agents. Here we discuss how cells in biofilms are affected by the presence of antibiotics at subinhibitory concentrations, and the possible ramifications of such secondary effects for healthcare and the environment.

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Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function

Cited by 73 publications

References 47 publications

Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages

Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages

Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level

Secondary Effects of Antibiotics on Microbial Biofilms

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