Breaking seed dormancy in European rowan seeds and its implications for regeneration

Afroze, Farhana; O'Reilly, Conor

doi:10.1007/s11056-013-9362-z

Cited by 12 publications

(17 citation statements)

References 13 publications

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“…Also, the MC of seeds (1.7 and 10.4% S3). Since there is no evidence for the occurrence of after-ripening during storage at 40 C, we suggest that the warm treatment may contribute to the breakdown of the seed coat and release of seed coat-imposed physical dormancy resulting in higher germination after subsequent cold stratification to release physiological dormancy (Afroze & O'Reilly, 2013). Similarly, higher germination after storage at ultra-low temperatures, for example, À196 C could result from scarification of the seeds (Kushnarenko et al, 2010) as seed coat removal is known to release physical dormancy of Sorbus domesticana L. (Prknov a, 2015).…”

Section: Hierarchical Cluster Analysis Of Emitted Volatilesmentioning

confidence: 87%

Volatile signature indicates viability of dormant orthodox seeds

Michalak

Plitta-Michalak

Jayanthi

et al. 2021

Physiologia Plantarum

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All seeds eventually die even under optimal storage conditions. The moment of viability loss is difficult to predict and detect. In order to differentiate between dead and viable dormant orthodox seeds, GC-MS analysis was used to non-invasively evaluate the volatile signature of seeds of Pyrus communis L. and Sorbus aucuparia L. Dormant seeds are capable of extended metabolic depression. However, their low metabolic rate remains largely unquantified, and there are no measurements of metabolites, i.e. volatile organic compounds (VOC) for physiologically dormant seeds during storage. Therefore, to address this issue, seeds were stored at a broad range of moisture content (MC) ranging from 2 to 30% under cryogenic (À196 C), cool (5 C) and elevated (40 C) temperatures. Volatile emission was highly dependent on seed MC and storage temperature and was higher under conditions associated with seed viability loss. However, changes in the emission of volatiles entrapped in seeds and released during 24 h after storage were detected for all conditions, providing insight into the processes occurring in dry dormant seeds. Among the 36 volatiles identified, three (acetaldehyde, ethanol, ethyl acetate) were highly correlated with seed germinability and show potential for the non-invasive screening of viability. Significantly, all three VOC are derived mostly from glycolysis and peroxidation and were detected even under very low moisture and temperature storage conditions. This is the first study to report on VOC accumulation and emission from physiologically dormant seeds and provide a broader view into their viability.

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Section: Hierarchical Cluster Analysis Of Emitted Volatilesmentioning

confidence: 87%

Volatile signature indicates viability of dormant orthodox seeds

Michalak

Plitta-Michalak

Jayanthi

et al. 2021

Physiologia Plantarum

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“…In this study, one month of warm stratification plus cold stratification is superior to cold stratification alone with no previous warm treatment. Because seeds of some Sorbus species are also associated with a mechanical dormancy as a result of a hard seed coat [11,28], warm stratification can contribute to breaking down this hard seed coat. Thus, a short warm stratification before cold stratification was proposed to increase the germination percentage of S. alnifolia seeds.…”

Section: Discussionmentioning

confidence: 99%

“…Further, its flowers make this tree one of the most aesthetically pleasing, which is undoubtedly one of the additional reasons why it is frequently cultivated by humans. However, most natural and semi-natural forests that have S. alnifolia populations are declining, which can be attributed to anthropogenic disturbance; therefore, restoration efforts are required in numerous countries worldwide [11].…”

Section: Introductionmentioning

confidence: 99%

Dormancy-Breaking and Germination Requirements for Seeds of Sorbus alnifolia (Siebold & Zucc.) K.Koch (Rosaceae), a Mesic Forest Tree with High Ornamental Potential

Tang

Zhang

Zhen

et al. 2019

Forests

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Sorbus alnifolia (Siebold & Zucc.) K.Koch (Rosaceae) is an economically important tree in the temperate forests of Eastern China. In recent decades, ever-increasing use and modification of forestlands have resulted in major degeneration of the natural habitat of S. alnifolia. Moreover, S. alnifolia seeds germinate in a complicated way, leading to a high cost of propagation. The current study aimed to determine the requirements for breaking seed dormancy and for germination as well as to characterize the type of seed dormancy present in this species. Moreover, the roles of temperature, cold/warm stratification, and gibberellic acid (GA3) in breaking dormancy were tested combined with a study of the soil seed bank. The results showed that intact seeds of S. alnifolia were dormant, requiring 150 days of cold stratification to achieve the maximum germination percentage at 5/15 °C. Exposure of the seeds to ranges of temperatures at 15/25 °C and 20/30 °C resulted in secondary dormancy. Scarifying seed coat and partial removal of the cotyledon promoted germination. Compared with long-term cold stratification, one month of warm stratification plus cold stratification was superior in breaking dormancy. Application of GA3 did not break the dormancy during two months of incubation. Seeds of S. alnifolia formed a transient seed bank. The viability of freshly matured S. alnifolia seeds was 87.65% ± 11.67%, but this declined to 38.25% after 6-months of storage at room temperature. Seeds of S. alnifolia have a deep physiological dormancy; cold stratification will be useful in propagating this species. The long chilling requirements of S. alnifolia seeds would avoid seedling death in winter.

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“…At the start of experiment, all seeds to be chilled for any duration before germination testing were adjusted to a target moisture content of 30%. This is the moisture content at which seeds of P. sylvestris are optimally chilled (Afroze and O'Reilly, 2013). Target moisture content allows moist seeds to break dormancy and do not allow any radicle growth.…”

Section: Seed Treatment: Target Moisture Content and Chillingmentioning

confidence: 99%

“…Once the target moisture content (30±2 %) was achieved the polythene bags containing the seeds were loosely tied to allow gas exchange but restrict water loss and transferred to a walk-in cold dark room set at 4±1°C for the required chilling duration. The seeds were inspected and gently shaken once in a week to allow uniform imbibition and air circulation (Afroze and O'Reilly, 2013).…”

Section: Seed Treatment: Target Moisture Content and Chillingmentioning

confidence: 99%

Moisture requirements for Pinus sylvestris seeds: Variation in germination among provenances from different elevations

Rampart¹

2019

Int. J. Biodivers. Conserv.

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Patterns of climatic variables are likely to change significantly in the coming century, with important but poorly-understood consequences for plant communities. This experiment determined how seeds of P. sylvestris collected from different elevations in two geographical regions performed when placed in simulated moisture stress conditions. Eight provenances from two regions (Norway and France) were chilled for zero and four weeks at 4±1°C and then germinated at 10 and 20°C, at water potentials of-0.3,-0.6 and-0.9 MPa. Moisture stress resulted in delayed germination and reduced germination capacity, and there was no germination at a water potential of-0.9 MPa. Provenances from high elevations were the most susceptible to moisture stress.

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Breaking seed dormancy in European rowan seeds and its implications for regeneration

Cited by 12 publications

References 13 publications

Volatile signature indicates viability of dormant orthodox seeds

Volatile signature indicates viability of dormant orthodox seeds

Dormancy-Breaking and Germination Requirements for Seeds of Sorbus alnifolia (Siebold & Zucc.) K.Koch (Rosaceae), a Mesic Forest Tree with High Ornamental Potential

Moisture requirements for Pinus sylvestris seeds: Variation in germination among provenances from different elevations

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