Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin

Twala, Pontsho Patricia; Mitema, Alfred; Baburam, Cindy; Feto, Naser Aliye

doi:10.3934/microbiol.2020020

Cited by 30 publications

(11 citation statements)

References 59 publications

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“…Dyp is another heme-containing peroxidase that can degrade various dyes, lignin-related compounds, and multiple mycotoxins and has drawn tremendous interest for biotechnological applications [ 25 ]. Therefore, the Bs Dyp was chosen as the first target protein to evaluate the potential of Ec -M13 strain, the high-heme-producing E. coli recombinant, for heme protein synthesis.…”

Section: Resultsmentioning

confidence: 99%

Engineering Escherichia coli for efficient assembly of heme proteins

Wang

Bai

et al. 2023

Microb Cell Fact

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Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile and have widespread applications in the fields of food, healthcare, medical and biological analysis. As a cofactor, heme availability plays a pivotal role in proper folding and function of heme proteins. However, the functional production of heme proteins is usually challenging mainly due to the insufficient supply of intracellular heme. Results Here, a versatile high-heme-producing Escherichia coli chassis was constructed for the efficient production of various high-value heme proteins. Initially, a heme-producing Komagataella phaffii strain was developed by reinforcing the C4 pathway-based heme synthetic route. Nevertheless, the analytical results revealed that most of the red compounds generated by the engineered K. phaffii strain were intermediates of heme synthesis which were unable to activate heme proteins. Subsequently, E. coli strain was selected as the host to develop heme-producing chassis. To fine-tune the C5 pathway-based heme synthetic route in E. coli, fifty-two recombinant strains harboring different combinations of heme synthesis genes were constructed. A high-heme-producing mutant Ec-M13 was obtained with negligible accumulation of intermediates. Then, the functional expression of three types of heme proteins including one dye-decolorizing peroxidase (Dyp), six oxygen-transport proteins (hemoglobin, myoglobin and leghemoglobin) and three CYP153A subfamily CYP enzymes was evaluated in Ec-M13. As expected, the assembly efficiencies of heme-bound Dyp and oxygen-transport proteins expressed in Ec-M13 were increased by 42.3–107.0% compared to those expressed in wild-type strain. The activities of Dyp and CYP enzymes were also significantly improved when expressed in Ec-M13. Finally, the whole-cell biocatalysts harboring three CYP enzymes were employed for nonanedioic acid production. High supply of intracellular heme could enhance the nonanedioic acid production by 1.8- to 6.5-fold. Conclusion High intracellular heme production was achieved in engineered E. coli without significant accumulation of heme synthesis intermediates. Functional expression of Dyp, hemoglobin, myoglobin, leghemoglobin and CYP enzymes was confirmed. Enhanced assembly efficiencies and activities of these heme proteins were observed. This work provides valuable guidance for constructing high-heme-producing cell factories. The developed mutant Ec-M13 could be employed as a versatile platform for the functional production of difficult-to-express heme proteins.

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Section: Resultsmentioning

confidence: 99%

Engineering Escherichia coli for efficient assembly of heme proteins

Wang

Bai

et al. 2023

Microb Cell Fact

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“…Peroxidase (POD) is one of the most widely used enzymes in biotechnological research, which can be ubiquitously found in animals, plants, and microorganisms. [103,104] It catalyzes various oxidation reactions by using H 2 O 2 or other substrates as electron donors. Following the first discovery of the intrinsic PODlike activity of Fe 3 O 4 nanoparticles, the science of nanozymes has blossomed considerably.…”

Section: Peroxidase-like Activitymentioning

confidence: 99%

Engineering Single‐Atom Nanozymes for Catalytic Biomedical Applications

Yang

Liao

Zou

et al. 2023

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Nanomaterials with enzyme‐mimicking properties, coined as nanozymes, are a promising alternative to natural enzymes owing to their remarkable advantages, such as high stability, easy preparation, and favorable catalytic performance. Recently, with the rapid development of nanotechnology and characterization techniques, single atom nanozymes (SAzymes) with atomically dispersed active sites, well‐defined electronic and geometric structures, tunable coordination environment, and maximum metal atom utilization are developed and exploited. With superior catalytic performance and selectivity, SAzymes have made impressive progress in biomedical applications and are expected to bridge the gap between artificial nanozymes and natural enzymes. Herein, the recent advances in SAzyme preparation methods, catalytic mechanisms, and biomedical applications are systematically summarized. Their biomedical applications in cancer therapy, oxidative stress cytoprotection, antibacterial therapy, and biosensing are discussed in depth. Furthermore, to appreciate these advances, the main challenges, and prospects for the future development of SAzymes are also outlined and highlighted in this review.

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“…Mn is required to build reactive centres in manganese peroxidase enzymes. These enzymes are produced by bacteria and fungi commonly cited in plastic studies (Twala et al, 2020), and have been shown to have an affinity for plastics (Enyoh et al, 2022), and are even suggested as potentially degradative towards PE (Twala et al, 2020).…”

Section: Mineral and Metal Association With Plasticmentioning

confidence: 99%

Microbe-mineral interactions in the Plastisphere: Coastal biogeochemistry and consequences for degradation of plastics

et al. 2023

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Microbe-mineral interactions, such as mineral substrate utilization and aggregate formation, have played a key role in the cycling of elements through Earth evolution. In water, soils, and sediment biogeochemistry modulates microbial community composition and mineral formation over spatial and temporal scales. Plastic is a new material that is now widespread in the environment. Both microbial and mineral associations with plastic comprise the Plastisphere, which influences the fate of plastic. This study focuses on how the biogeochemical environment defines microbial and mineral association with polyethylene (PE) and polystyrene (PS) over a 12-month period in a temperate coastal harbor. The coastal harbor environment was separated into 3 conceptual compartments defined by physical and biogeochemical conditions, that allow transfer of electrons between species e.g., light penetration and redox setting. Microbe and mineral association were investigated in the water column, top sediment, and bottom sediment by applying a range of modern analytical techniques to identify changes in the chemical structures of plastics, microbial community development, metal, salt and mineral formation. The epiplastic microbial community was distinct to that of the surrounding environment across changing redox conditions. The type and oxidation state of metallic minerals formed on plastics or entrapped in the biofilm matrix related to the dominant abiotic and biotic processes across redox conditions. FTIR spectroscopy indicated the occurrence of PE and PS oxidation in the various biogeochemical environments. Combined, these findings demonstrate that redox conditions and surrounding biogeochemistry mediate the composition of mineralogical and biological loading of PE and PS in coastal marine environments. This suggests that the biogeochemical setting in which the plastics are stored constrains the development of plastic interfacial biogeochemistry and the potential for plastic degradation and transport over time.

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Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin

Cited by 30 publications

References 59 publications

Engineering Escherichia coli for efficient assembly of heme proteins

Engineering Escherichia coli for efficient assembly of heme proteins

Engineering Single‐Atom Nanozymes for Catalytic Biomedical Applications

Microbe-mineral interactions in the Plastisphere: Coastal biogeochemistry and consequences for degradation of plastics

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