This paper aims to optimize the running posture of college students by using the principle of rod model in biomechanics. Based on this, through experiments and analysis, this paper studies some posture problems existing in college students' running process, and analyzes the influence these problems may have on the speed and stability of college students, and finally puts forward some solutions. In this paper, firstly, through the analysis of college students' motion images in Sports-1M data set, the movement process of college students is grasped, and a biomechanical model of college students' running posture is established. The actual running posture of college students is compared with this model. Then, in the process of comparison, the paper finds some posture defects, such as the backward landing point of footsteps, too great knee flexion and so on, which may lead to extra friction and energy loss for college students and have a great impact on their sports process. Therefore, this paper uses the image data in the data set to detect the movement state of college students, and finds that the running posture of some college students has a significant impact on their running process, so this paper makes a series of adjustments and optimizations to the running process of college students. It includes the adjustment of running posture, such as the adjustment of knee joint and elbow joint, which provides reasonable suggestions for improving the speed and stability of college students to effectively reduce the pressure on college students' knee joint and other related parts. In addition, the paper also tests the muscle electrical signals and skin reactions of college students during exercise. The results show that the optimized posture can make more effective use of muscle strength and reduce skin friction and wear during simulated running. This paper provides new ideas and examples for the optimization of college students' running posture, and also provides reference for the application and exploration of biomechanics in sports training. In addition, the paper can improve the competitive level of college students and make some contributions to the development of sports.