Abstract. Breast thermography is a promising technique allowing breast cancer detection with the aid of infrared technology. However, the automatic segmentation of the regions of interest (ROI) to be analyzed is a difficult task and, thus, it is not commonly performed. In this paper we propose an automated technique for ROI extraction. The algorithm uses Canny edge detection with automatic threshold and symmetry inspection in order to correct tilt errors and to define a symmetry axis in the image. Furthermore, the Hough transform is used to provide circles giving a favorable approximation to the ROI. A region-based active contours technique and an edge smoothing technique are also used to improve the previous segmentation, taking in consideration the energies of the images. Experimental results indicate that the ROI were extracted more accurately and with higher precision than a state-of-the-art method.