Background: All parents of a child know that keeping their child at home is difficult enough, but when the child goes to school, there are new challenges and very dangerous situation. Preparing children for such situations is one of the tasks of the education system. The purpose of this study was to investigate the efficacy of operational first aid training course in preschool children. Methods: This study is a baseline of single-group experiences. The sample size included 150 children who were randomly selected from kindergarten. They underwent a course of first aid training and the results were evaluated 3 weeks after training. Results: Both quantitative and qualitative improvements were observed in the childrenchr('39')s efficacy in giving first aid. For example, the assessment of consciousness status increased from 51.3% before training to 64% after training or nasal bleeding control increased from 34% before training to 89.3% after training. Conclusion: First aid training courses help children get immediate and adequate help in situations emergencies. The children can learn the basic aspects of first aid and these skills increasing self-care in children. So the first aid teaching program should include the transfer of knowledge and should also motivate children to perform first aid and develop their helping aptitude.