Breast Tissue Reconstruction Using Polycaprolactone Ball Scaffolds in a Partial Mastectomy Pig Model

Shim, Kyu-Sik; Ryu, Da Hye; Jo, Han-Saem; Kim, Ki-Bum; Kim, Dohyun; Park, Yong-Kyu; Heo, Min; Cho, Hee-Eun; Yoon, Eul Sik; Lee, Won Jai; Roh, Tai Suk; Song, Seung Yong; Baek, Wooyeol

doi:10.1007/s13770-023-00528-x

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…This is largely due to the porous structure of our scaffolds, which was large enough to encourage tissue integration rather than peripheral fibrous capsule formation [34]. This contrasts with other studies using implants with smaller pores, which have resulted in peripheral fibrous encapsulation [17,18,28]. In addition, we identified that the scaffold FBR resulted in a rich ECM network containing blood vessels, essentially creating an internal vascularized structure around mPCL struts, allowing for tissue regeneration.…”

Section: Discussionmentioning

confidence: 81%

“…In contrast, scaffolds filled with 50 mL of autologous fat graft regenerated 59.5 ± 4.6 mL (95% CI), representing a modest 20% increase in soft tissue, but this did not account for potential fat graft resorption, which may increase that gain. In other large animal studies, Shim et al [ 28 ] were also able to regenerate de-novo soft tissue utilizing empty PCL scaffolds designed in a ball-like shape in four pigs over three months. However, their scaffolds were significantly smaller (3 cm in diameter equating to 14 mL volume) compared to our volumes (100 mL), limiting their clinical applicability.…”

Section: Discussionmentioning

confidence: 99%

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Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Cheng,

Janzekovic,

Finze

et al. 2024

Bioengineering

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Scaffold-guided breast tissue regeneration (SGBTR) can transform both reconstructive and cosmetic breast surgery. Implant-based surgery is the most common method. However, there are inherent limitations, as it involves replacement of tissue rather than regeneration. Regenerating autologous soft tissue has the potential to provide a more like-for-like reconstruction with minimal morbidity. Our SGBTR approach regenerates soft tissue by implanting additively manufactured bioresorbable scaffolds filled with autologous fat graft. A pre-clinical large animal study was conducted by implanting 100 mL breast scaffolds (n = 55) made from medical-grade polycaprolactone into 11 minipigs for 12 months. Various treatment groups were investigated where immediate or delayed autologous fat graft, as well as platelet rich plasma, were added to the scaffolds. Computed tomography and magnetic resonance imaging were performed on explanted scaffolds to determine the volume and distribution of the regenerated tissue. Histological analysis was performed to confirm the tissue type. At 12 months, we were able to regenerate and sustain a mean soft tissue volume of 60.9 ± 4.5 mL (95% CI) across all treatment groups. There was no evidence of capsule formation. There were no immediate or long-term post-operative complications. In conclusion, we were able to regenerate clinically relevant soft tissue volumes utilizing SGBTR in a pre-clinical large animal model.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Cheng,

Janzekovic,

Finze

et al. 2024

Bioengineering

View full text Add to dashboard Cite

show abstract

“…This is largely due to the porous structure of our scaffold which was large enough to encourage tissue integration, rather than peripheral fibrous capsule formation [32]. This contrasts with other studies using implants with smaller pores resulting in peripheral fibrous encapsulation [17,18,26]. In addition, we identified the scaffold FBR resulted in a rich ECM network containing blood vessels, essentially creating an internal vascularized structure around mPCL struts allowing for tissue regeneration.…”

Section: Groupsmentioning

confidence: 82%

“…In contrast, scaffolds filled with 50 ml of autologous fat graft regenerated 59.5  4.6 mL (95% CI), representing a modest 20% increase in soft tissue, but this did not account for potential fat graft resorption which may increase that gain. In other large animal studies, Shim et al [26] were also able to regenerate de-novo soft tissue utilizing empty PCL scaffolds designed in a ball-like shape in four pigs over three months. However, their scaffolds were significantly smaller (3cm in diameter equating to 14 ml volume) compared to our volumes (100 ml), limiting their clinical applicability.…”

Section: Discussionmentioning

confidence: 96%

Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Cheng,

Janzekovic,

Finze

et al. 2024

Preprint

View full text Add to dashboard Cite

Scaffold guided breast tissue regeneration (SGBTR) can both transform reconstructive and cosmetic breast surgery. Implant-based surgery is the most common method. However, there are inherent limitations as there is replacement of tissue rather than regeneration. Regenerating autologous soft tissue has the potential to provide a more like-for-like reconstruction with minimal morbidity. Our SGBTR approach regenerates soft tissue by implanting additively manufactured bioresorbable scaffolds filled with autologous fat graft. A pre-clinical large animal study was conducted implanting 100 ml breast scaffolds (n=55) made from medical-grade polycaprolactone in 11 minipigs for 12 months. Various treatment groups were investigated where immediate or delayed autologous fat graft, as well as platelet rich plasma were added to scaffolds. Computed Tomography and Magnetic Resonance Imaging was performed on explanted scaffolds to determine volume and distribution of regenerated tissue. Histological analysis was performed to confirm tissue type. At 12 months we were able to regenerate and sustain a mean soft tissue volume of 60.9 4.5 ml (95% CI) across all treatment groups. There was no evidence of capsule formation. There were no immediate or long-term post-operative complications. In conclusion, we were able to regenerate clinically relevant soft tissue volumes utilizing SGBTR in a pre-clinical large animal model.

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Advanced 3D Cell Culture Platform for Tissue Engineering

Yoon

2023

Tissue Eng Regen Med

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Breast Tissue Reconstruction Using Polycaprolactone Ball Scaffolds in a Partial Mastectomy Pig Model

Cited by 4 publications

References 32 publications

Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Conceptualizing Scaffold Guided Breast Tissue Regeneration in a Preclinical Large Animal Model

Advanced 3D Cell Culture Platform for Tissue Engineering

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