Breast Tumor Targeting with PAMAM-PEG-5FU-99mTc As a New Therapeutic Nanocomplex: In In-vitro and In-vivo studies

Narmani, Asghar; Arani, Monire Alsadat Afzali; Mohammadnejad, Javad; Vaziri, Ali; Solymani, Sedigheh; Yavari, Kamal; Talebi, Fatemeh; Darzi, Simin Janitabar

doi:10.1007/s10544-020-00485-5

Cited by 48 publications

(18 citation statements)

References 34 publications

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“…25 On the other hand, FA-CS-miR-126, the main therapeutic nano-complex which has also considered an efficient drug delivery system in this study, is demonstrated good cytotoxicity on cancer cells; as 56.67 ± 3.12%, 50.24 ± 3.34%, and 40.7 ± 2.98% of cell viability attained for A549 at 24, 48, and 72 h of treatment for 500 nM while these values were 85.52 ± 4.51%, 84.41 ± 4.23%, and 75.14 ± 4.7% for MRC5 cell line at the same circumstances which are indicating excellent internalization efficiency of FA-decorated CS and great therapeutic and cytotoxicity of miR-126 as cancer cell growth inhibitor. 20,26 The FA-CS-S-miR derivative did not demonstrate considerable cytotoxicity on cancer cells which further verify miR-126 tumor inhibitor performance. The IC50 dose (a dose that kills 50% of cells) was obtained for FA-CS-miR-126 nano-complex for a concentration of 500 nM at 48 and 72 h of post-treatment.…”

Section: Cell Viability Assaymentioning

confidence: 65%

“…For FA-CS nano-complex, MRC5 cell viability values were respectively obtained 90.94 ± 4.79%, 92.76 ± 4.82%, and 85.45 ± 4.36% for 125 nM at 24, 48, and 72 h of post-treatment whereas these amounts were 83.60 ± 5.02%, 86.94 ± 4.78%, and 80.68 ± 3.86% at the same conditions which are indicating FA receptormediated internalization of FA-CS in cancer cells since this receptor has been significantly overexpressing on the surface of cancer cells which, in turn, improve high values of cellular uptake and result in the high level of accumulation and cytotoxicity. 17,18,20 Furthermore, CS-miR-126 nano-complex, as non-target therapeutic nanocomposite, exhibited high cell toxicity at a concentration of 500 nM after 72 h of treatment that was 69.32 ± 4.38% and 64.76 ± 3.99% for MRC5 and A549 cell lines, respectively (Figure 4(c) and (f)). These values approve better cell death activity of miR-126-entrapped CS derivative when it is compared with CS-S-miR as control.…”

Section: Cell Viability Assaymentioning

confidence: 97%

“…whereas it is highly restricted in normal human cells. 19,20 FA can covalently bind to FR on cancer cells and induce endocytosis.…”

Section: Introductionmentioning

confidence: 99%

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Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy

Golafzani

Vaziri

Javanmardi

et al. 2022

Journal of Bioactive and Compatible Polymers

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Objective: Nanoparticle-based drug delivery systems (DDSs) have been playing a considerable role in the eradication of cancer. In this experimental study, we designed and synthesized folic acid (FA)-decorated chitosan (CS) nanocarrier for targeted delivery of miR-126 (as a therapeutic agent) to lung cancer A549 cells. Materials and methods: Therefore, the FA-CS-miR-126 nano-complex was perfectly developed and characterized by various analytical devices such as Fourier transform infrared (FT-IR) and dynamic light scattering (DLS) spectroscopies and as well as transmission electron microscopy (TEM). The size was determined lower than 100 nm for synthetics. Then, a gel retardation assay was performed to investigate the entrapment efficiency of nano-complex. Afterward, the sort of in vitro assays was implemented on A549 (FA receptor-positive lung cancer cell line) and MRC5 (normal human diploid cell line) to evaluate the therapeutic efficiency of FA-CS-miR-126. Results: As the cell viability (40.7 ± 2.98% cell viability after 72 h treatment with 500 nM), migration assay (weaker migration after 24 h and 48 h), apoptotic and autophagy genes expression level (Caspse9: sixfolds; BAX: 17 folds; ATG5: fourfolds; and BECLIN1: threefolds more than the control group), the reduced expression level of EGF-L7, as a target gene for miR-126 was evaluated by Real-Time PCR too, then, cell cycle arrest (8.66% of cells in sub-G1 phase), and cell apoptosis assay (21.0% of cancer cell in late apoptosis phase) were scrutinized. Conclusion: These results are remarkably approved the biocompatible and efficient performance of FA-CS-miR-126 as a promising DDS.

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Section: Cell Viability Assaymentioning

confidence: 65%

Section: Cell Viability Assaymentioning

confidence: 97%

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Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy

Golafzani

Vaziri

Javanmardi

et al. 2022

Journal of Bioactive and Compatible Polymers

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“…Their evaluation of cellular uptake using confocal microscopy has demonstrated that folic acid-functionalized nanocarrier could be remarkably internalized to breast cancer cells via folate receptor-mediated uptake [51]. Targeted drug delivery plays a crucial role in the suppression and tracing of cancer cells since using this type of treatment modality can not only potentially minimize the side effect of therapeutics in the whole human body but also reduce the treatment period and cost of treatment in patients with cancer [52].…”

Section: Confocal Cell Imaging and Nanoparticle Cellular Uptakementioning

confidence: 99%

Folic Acid-decorated and PEGylated Graphene Quantum Dots as efficient Tamoxifen delivery system against breast cancer cells: in vitro studies

Amraee

Majd

Torbati

et al. 2023

Preprint

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Nano-drug delivery systems have provided a platform for improving the formulations of targeted cancer therapy. Graphene quantum dots (GQDs) possess potential properties for the targeted transport and tracking of anticancer medication to tumor cells. For this purpose, the synthesized GQDs were decorated with folic acid for better binding to folate-positive breast cancer cells and methoxy polyethylene glycol (mPEG2000) for a longer lifetime, and finally loaded with anticancer drug tamoxifen (TMX). The synthesized nano-drugs were characterized using FTIR, XRD, UV-vis, PL, HRTEM, FESEM, and DLS analytical devices. Based on the obtained data, the average hydrodynamic diameter of particles dissolved in water was 294.7 nm and the size of dehydrated particles was between 3 and 153 nm. PL data showed that prepared nanoparticles have an emission wavelength of 438 nm, which is in the blue fluorescent wavelength range, thus making these nanoparticles suitable for cell imaging. The encapsulation efficiency and loading percentage of tamoxifen in nanoparticles were calculated at about 52.5% and 30%, respectively, and cumulative drug release reached 89% within 42 hours. The cytotoxicity effects of nanoparticles on MCF-7 cell line were investigated. The targeted mPEG-GQDs-FA/TMX (TMX-FPGs) nano-drug was more toxic than free TMX drug on MCF-7 cells and had reduced side effects on healthy HDF cells. TMX-FPGs induced apoptosis cell death in cancer cells, significantly. The confocal cell imaging experiments showed that TMX-FPGs are appropriate for monitoring and targeting MCF-7 cancer cells. These data have approved the potency of TMX-FPGs against breast cancer cells.

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“…In vivo studies in breast tumour-bearing BALB/C mice resulted in excellent tumour inhibition rate (reduced growth), significant reduction of tumour size and prolonged survival time. Furthermore, data obtained after testing with a gamma camera indicated that nanocomplex can be useful as an imaging tool [105,106].…”

Section: Fa-targeted Nps For Thermo- Photo- Radiotherapy and Diagnomentioning

confidence: 99%

Single- versus Dual-Targeted Nanoparticles with Folic Acid and Biotin for Anticancer Drug Delivery

et al. 2021

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Cancer is one of the major causes of death worldwide and its treatment remains very challenging. The effectiveness of cancer therapy significantly depends upon tumour-specific delivery of the drug. Nanoparticle drug delivery systems have been developed to avoid the side effects of the conventional chemotherapy. However, according to the most recent recommendations, future nanomedicine should be focused mainly on active targeting of nanocarriers based on ligand-receptor recognition, which may show better efficacy than passive targeting in human cancer therapy. Nevertheless, the efficacy of single-ligand nanomedicines is still limited due to the complexity of the tumour microenvironment. Thus, the NPs are improved toward an additional functionality, e.g., pH-sensitivity (advanced single-targeted NPs). Moreover, dual-targeted nanoparticles which contain two different types of targeting agents on the same drug delivery system are developed. The advanced single-targeted NPs and dual-targeted nanocarriers present superior properties related to cell selectivity, cellular uptake and cytotoxicity toward cancer cells than conventional drug, non-targeted systems and single-targeted systems without additional functionality. Folic acid and biotin are used as targeting ligands for cancer chemotherapy, since they are available, inexpensive, nontoxic, nonimmunogenic and easy to modify. These ligands are used in both, single- and dual-targeted systems although the latter are still a novel approach. This review presents the recent achievements in the development of single- or dual-targeted nanoparticles for anticancer drug delivery.

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Breast Tumor Targeting with PAMAM-PEG-5FU-99mTc As a New Therapeutic Nanocomplex: In In-vitro and In-vivo studies

Cited by 48 publications

References 34 publications

Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy

Delivery of miRNA-126 through folic acid-targeted biocompatible polymeric nanoparticles for effective lung cancer therapy

Folic Acid-decorated and PEGylated Graphene Quantum Dots as efficient Tamoxifen delivery system against breast cancer cells: in vitro studies

Single- versus Dual-Targeted Nanoparticles with Folic Acid and Biotin for Anticancer Drug Delivery

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