Breath test measurements in combination with indirect calorimetry for estimation of 13C-leucine oxidation in mink (Mustela vison)

Tauson, Anne‐Helene; Ali, Amir; Kanska, Katarzyna; Sobczynska, Katarzyna; Chwalibog, A.

doi:10.1016/s0040-6031(99)00496-7

Cited by 4 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As can be seen on these figures, a detectable increase in labelled CO 2 could already be measured 15 min postadministration. A similar rapid first appearance of labelled CO 2 was also observed with adult rats (Schreurs et al., 1992) and mink (Tauson et al., 2000, 2001) injected intraperitoneally with 1‐ 14 C‐ and 1‐ 13 C‐labelled leucine respectively. This rapid increase was also noticed during breath tests with human subjects after ingestion of 13 C‐labelled leucine, lysine or methionine (Schreurs and Krawielitzki, 2003).…”

Section: Resultssupporting

confidence: 72%

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Swennen

Laroye

Janssens

et al. 2007

Animal Physiology Nutrition

View full text Add to dashboard Cite

Research has shown that broiler chickens reared on a low-protein diet have a more efficient protein digestion. However, information on the fate of absorbed amino acids in relation to the dietary crude protein level in poultry is sparse. Therefore, this study aimed at developing a methodology for a 1-(13)C(1)-leucine breath test combined with indirect calorimetry, and to apply this technique using broiler diets known to induce differences in protein retention. From 14 days of age onwards, broiler chickens were reared on one of two isocaloric diets with substitutions between fat and protein [low-protein (LP) vs. high-protein (HP) diet: 130.4 vs. 269 g protein/kg; and 101.8 vs. 27.9 g fat/kg]. Every 4 or 5 days, three chickens per diet were placed in the respiratory cells for 48 h. The broilers were intubated with 40 mg 1-(13)C(1)-leucine/kg body weight, followed by breath sampling for 4 h at 15-min intervals and mass spectrometric analysis of the (13)C:(12)C ratio in the samples. The CO(2) level in the respiratory cell air was monitored and excreta samples were collected. The methodology to study l[1-(13)C(1)]leucine decarboxyation in chickens using a breath test combined with indirect calorimetry was accomplished. Results of the nitrogen balance test indicated that the LP broilers had an improved dietary protein retention compared with the HP animals. Moreover, LP chickens decarboxylated a significantly lower percentage of l[1-(13)C(1)]leucine, demonstrating several 'protein- or amino acid-sparing' mechanisms in animals reared on a diet with lower protein level, both at the digestive and at the postabsorptive level.

show abstract

Section: Resultssupporting

confidence: 72%

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Swennen

Laroye

Janssens

et al. 2007

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

Body temperature and heat production in suckling rat endotoxaemia: Beneficial effects of glutamine

Garrett-Cox

Pierro

Spitz

et al. 2003

Journal of Pediatric Surgery

View full text Add to dashboard Cite

Net Protein Oxidation Is Adapted to Dietary Protein Intake in Domestic Cats (Felis silvestris catus)

Russell¹,

Murgatroyd

Batt³

2002

The Journal of Nutrition

View full text Add to dashboard Cite

Cats have a requirement for dietary protein two to three times that of omnivores and herbivores. This was reported to be due to the hepatic catabolic enzymes of this species being set to a permanently high level and, therefore, showing little adaptation to low dietary protein. A major mechanism for adapting to dietary protein in other species is amino acid oxidation (hereafter referred to as protein oxidation), and the objective of this study was to determine whether protein oxidation in cats was correlated with protein intake. Net protein and net fat oxidation in six adult cats were studied directly from gas exchanges using indirect calorimetry, after feeding moderate protein (MP; 35% energy) and high protein (HP; 52% energy) diets. Protein oxidation was significantly higher (P < 0.05) when cats were fed the HP diet (28.4 plus minus 0.7 mg/min) rather than the MP diet (20.4 plus minus 0.8 mg/min). Fat oxidation was significantly higher (P < 0.05) when cats consumed the MP diet (9.0 plus minus 0.7 mg/min) rather than the HP diet (4.7 plus minus 0.5 mg/min). Protein oxidation was significantly correlated (linear regression, R(2) = 46.0, P < 0.05) with protein intake such that the mean ratio of 18-h oxidation: 18-h intake was 1.2 on both diets. Fat oxidation was significantly correlated (linear regression, R(2) = 18.9, P < 0.05) with fat intake such that the mean ratio of 18-h fat oxidation: 18-h fat intake was 1.1 (MP) and 0.9 (HP). This study demonstrated that cats adapt net protein oxidation at these levels of protein intake, and the reason for the high dietary protein requirement of this species is, therefore, unclear.

show abstract

Breath test measurements in combination with indirect calorimetry for estimation of 13C-leucine oxidation in mink (Mustela vison)

Cited by 4 publications

References 18 publications

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Body temperature and heat production in suckling rat endotoxaemia: Beneficial effects of glutamine

Net Protein Oxidation Is Adapted to Dietary Protein Intake in Domestic Cats (Felis silvestris catus)

Contact Info

Product

Resources

About

Breath test measurements in combination with indirect calorimetry for estimation of 13C-leucine oxidation in mink (Mustela vison)

Cited by 4 publications

References 18 publications

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐13C1]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐13C1]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Body temperature and heat production in suckling rat endotoxaemia: Beneficial effects of glutamine

Net Protein Oxidation Is Adapted to Dietary Protein Intake in Domestic Cats (Felis silvestris catus)

Contact Info

Product

Resources

About

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Rate of metabolic decarboxylation of leucine as assessed by a l[1‐¹³C₁]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio