Breed and Swine Lymphocyte Antigen Haplotype Differences in Agglutination Titers Following Vaccination with B. Bronchiseptica2

Abstract

Genetic differences in immune response to B. bronchiseptica after vaccination with a commercial B. bronchiseptica bacterin were investigated in 1,069 8-wk-old pigs. These pigs were from 65 litters born in the spring and 66 litters born in the fall of 1982 and were purebreds from the Chester White (n = 128), Duroc (n = 281), Hampshire (n = 143), Landrace (n = 309) and Yorkshire (n = 208) breeds. Each litter was raised separately. Individual pigs were vaccinated im at 4 and 6 wk of age with 2 ml of B. bronchiseptica bacterin. At 8 wk of age, 8 ml of blood were collected from each animal and serum prepared to determine agglutinating antibody titers against B. bronchiseptica bacterin by a bacterial agglutination method. In addition, lymphocytes were separated from 1 ml of heparinized blood and used to determine Swine Lymphocyte Antigen (SLA) haplotypes by using cytotoxic antibodies against the SLA complex. Antisera for 3 SLA haplotypes were made available by the National Institutes of Health. Results indicated that breed of pig (P<.01) and dam of pig (P<.01) affected the immune response of the pig after B. bronchiseptica vaccination. Higher immune response was also associated (P<.05) with one of the SLA haplotypes tested. Heritability estimates for immune response following vaccination were .10 ± .12 (half-sib) and .42 ± .19 (full-sib). Results suggest that the relationship of the SLA complex to immune response in the pig and nonadditive genetic and maternal effects on immune response should be further investigated. SummaryGenetic differences in immune response to B. bronchiseptica after vaccination with a commercial B. bronchiseptica bacterin were investigated in 1,069 8-wk-old pigs. These pigs were from 65 litters born in the spring and 66 litters born in the fall of 1982 and were purebreds from the Chester White (n = 128), Duroc (n = 281), Hampshire (n = 143), Landrace (n = 309) and Yorkshire (n = 208) breeds. Each litter was raised separately. Individual pigs were vaccinated im at 4 and 6 wk of age with 2 ml of B. bronchiseptica bacterin. At 8 wk of age, 8 ml of blood were collected from each animal and serum prepared to determine agglutinating antibody titers against B. bronchiseptica bacterin by a bacterial agglutination method. In addition, lymphocytes were separated from 1 ml of heparinized blood and used to determine Swine Lymphocyte Antigen (SLA) haplotypes by using cytotoxic antibodies against the SLA complex. Antisera for 3 SLA haplotypes were made available by the National Institutes of Health. Results indicated that breed of pig (P<.01) and dam of pig (P<.O1) affected the immune response of the pig after B. bronchb septica vaccination. Higher immune response was also associated (P<.05) with one of the SLA haplotypes tested. Heritability estimates

Genetic Control of Immune Response to Pseudorabies and Atrophic Rhinitis Vaccines: I. Heterosis, General Combining Ability and Relationship to Growth and Backfat

“…General combining abilities are in table 5 for this response, and these rank Yorkshire, Duroc, Landrace from highest to lowest. However, as previously discussed, the assays used to detect the antibodies differed in the two studies because the ELISA assay measured IgG antibodies and that of Rothschild et al (1984a) preferentially measured IgM. Ranking of breeds at 119 d placed Duroc as the lowest responder in this study.…”

“…Rothschild et al (1984b) showed titers to PR vaccine ranking Yorkshire, Duroc, Landrace from highest to lowest, which is the same as in table 5. Rothschild et al (1984a) showed titers to B. bronchiseptica vaccine at 56 d ranked Yorkshire, Landrace, Duroc from highest to lowest. General combining abilities are in table 5 for this response, and these rank Yorkshire, Duroc, Landrace from highest to lowest.…”

“…A primary component of disease resistance is the humoral immune response (Gavora and Spencer, 1983 ;Buschmann et al, 1985;Warner et al, 1987 (Buschman et al, 1974), hen egg white lysozyme (Vaiman et al, 1978), E. coli vaccine (Edfors-Lilja et al, 1984, 1985, Bordetella bronchiseptica vaccine (Rothschild et al, 1984a) and pseudorabies vaccine (Rothschild et al, 1984b). Little is known about the relationships among immune response traits and production traits (Rothschild, 1985;Edfors-Lilja et al, 1986).…”

Characterization and phylogenetic analysis of the swine leukocyte antigen 3 gene from Korean native pigs

“…Miniature pigs (MIPs) are excellent experimental animal models, and are ideal for studying SLA genotypes and alleles (Rothschild et al, 1984;Mallard et al, 1989). The Korean native pig (KNP) can be used as an animal model, even though its genetic variability has not been completely verified.…”

Phylogenetic analysis of the swine leukocyte antigen — 2 gene for Korean native pigs

“…Variation in swine leukocyte antigen (SLA) genes influences immune responses, organ and cell transplantation success, and disease resistances (Smith et al, 2004). Tissue transplantation studies using swine organs have mainly used inbred miniature pigs (MIP), which have been idealized to medical uses and immunological researches as excellent experimental animals with identifying SLA genotypes and alleles (Mallard et al, 1989;Rothschild et al, 1984). Within the swine MHC lies the SLA class I antigen region on S. scrofa chromosome 7q11-14, (Barbosa et al, 2004;Smith et al, 1995) containing SLA-1, SLA-2, and SLA-3 genes (Ando et al, 2003;Charon et al, 2000).…”