Breeding for biotic stress resistance in<i>Rhododendron simsii</i>

Huylenbroeck, Johan Van; Calsyn, E.; Keyser, Ellen De; Luypaert, Gil

doi:10.17660/actahortic.2015.1104.56

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Because field trials of woody ornamentals require a great deal of space and effort, a screening method in a seedling stage is beneficial. Examples of the use of such screening are found in several crops, such as maize (Mesterhazy, Lemmens, & Reis, 2012), wheat (Sip, Chrpova, & Stockova, 2011) and also in some ornamentals such as roses (Leus, Huylenbroeck, Bockstaele, & Höfte, 2008), Anthurium (Elibox & Umaharan, 2008) and Rhododendron (Van Huylenbroeck, Calsyn, Keyser, & Luypaert, 2015).…”

Section: Discussionmentioning

confidence: 99%

Breeding and selection of Buxus for resistance to Calonectria pseudonaviculata

Laere

Heungens

Gehesquière

et al. 2019

Journal of Phytopathology

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Box blight is a widespread disease of Buxus caused by the pathogen Calonectria pseudonaviculata. It is responsible for significant losses in nurseries, gardens and wild boxwood populations. Our goal was to maximize the efficiency of a breeding programme towards increased disease resistance. The use of artificial inoculation of young F1 seedlings with C. pseudonaviculata spores under greenhouse conditions appeared to be a reliable tool for early selection of interesting prebreeding material. Overall, the four hybrid populations screened showed a segregating behaviour between their parents when determining the percentage of diseased leaves and lesion diameter. Genotypes were also found with an increased tolerance as compared to the parental species. Approximately 50% of the seedlings had the same score for both parameters after artificial inoculation in the greenhouse and in the field. Of the seedlings that showed severe symptoms in the greenhouse, <15% showed no disease symptoms in the field. Therefore, for larger breeding programmes, we propose a two‐step selection procedure: first artificial inoculation at seedling level to eliminate all genotypes with severe symptoms and then evaluation of the remaining seedlings in the field. Using this strategy, we were able to select several genotypes in our four hybrid populations with improved resistance to C. pseudonaviculata.

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Section: Discussionmentioning

confidence: 99%

Breeding and selection of Buxus for resistance to Calonectria pseudonaviculata

Laere

Heungens

Gehesquière

et al. 2019

Journal of Phytopathology

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“…White mallow azaleas contain small amounts of delphinidin and cyanidin, whereas the petals of pink mallow azaleas have six times more cyanidin-3-O-rutinoside than those of purple mallow azaleas [ 13 ]. Most studies on Rhododendron plants have focused on the extraction of volatile components and triterpenoids from ocimene [ 14 ], genetic diversity [ 15 ], and translocation breeding and adversity stress [ 16 ]. However, none of these studies has investigated the metabolite composition in petals or explored the content of metabolites at different developmental stages, nor have they elucidated the mechanisms of gene regulation associated with flower color.…”

Section: Introductionmentioning

confidence: 99%

Insight into the Molecular Mechanism of Flower Color Regulation in Rhododendron latoucheae Franch: A Multi-Omics Approach

Xiao

Zhang

Liao

et al. 2023

Plants

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Rhododendron latoucheae Franch. (R. latoucheae) is a valuable woody plant known for its high ornamental value. While purple flowers are a distinct and attractive variant phenotype of R. latoucheae, the underlying mechanism regulating its flower color is still poorly understood. To investigate the molecular regulatory mechanism responsible for the variation in flower color, we selected plants with white-pink and purple petals as the object and conducted analyses of metabolites, key genes, and transcription factors associated with flower color. A combined metabolome–transcriptome analysis was performed, and the expression of key genes was subsequently verified through qRT-PCR experiments. The results of our study demonstrated a significant enrichment of differential metabolites in the flavonoid metabolic pathway. Changes in anthocyanin content followed the same trend as the observed flower color variations, specifically showing significant correlations with the contents of malvidin-3-O-glucoside, dihydromyricetin, gallocatechin, and peonidin-3-O-glucoside. Furthermore, we identified three key structural genes (F3GT1, LAR, ANR) and four transcription factors (bHLH130, bHLH41, bHLH123, MYB4) that are potentially associated with the biosynthesis of flavonoid compounds, thereby influencing the appearance of purple flower color in R. latoucheae.

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“…Differences in susceptibility to broad mites have been observed in pot azalea, along with the damage rate, and in relation to the population sizes of P. latus on the tested azalea cultivars [12]. Trichomes (plant hairs) are potentially involved in broad mite resistance in pot azalea [14]. Additionally, in Capsicum species, trichomes are known to act as a limiting factor for the development of broad mites [15].…”

Section: Introductionmentioning

confidence: 99%

Jasmonic Acid and Salicylic Acid Levels in Defense Response of Azalea (Rhododendron simsii Hybrid) to Broad Mite (Polyphagotarsonemus latus)

et al. 2022

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The broad mite (Polyphagotarsonemus latus (Banks)) is an important pest in many crops, including azalea (Rhododendron simsii Planch. hybrid). Broad mites cause the malformation of shoot tips, leaves and flowers in azalea. It is known that the jasmonic acid (JA)- and salicylic acid (SA)-dependent signaling pathways are related to the presence of herbivorous mites. Here, we describe the levels of the two main plant defense-related hormones, SA and JA, in mite-infested plants. The plant hormones were analyzed using liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). We studied both short-term hormonal responses under controlled conditions with artificial inoculation, and long-term responses under culture conditions with natural infestation. The long-term development of broad mite populations and hormone response were studied during two subsequent growing seasons on 3 and 18 different cultivars, respectively. During the experiments on 18 azalea cultivars under natural infestation, the presence of different species of tarsonemid mites was also examined. JA concentrations only showed variation in the early phase of infestation. Subsequently, the SA levels increased significantly for all the cultivars where broad mites were detected. Based on the observed timing of the defense responses, we suggest that the interaction of the JA and SA pathways as a defense response for pot azalea against P. latus involves a primary plant response through the JA pathway. In the presence of the mites, the production of SA increased in the plants in a later phase as the P. latus population grew. Our results also show that the hormone response depends on type of mite. Changes in hormone levels were found upon infestation with P. latus, but not in the presence of another frequently occurring tarsonemid mite, Tarsonemus confusus Ewing.

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Breeding for biotic stress resistance inRhododendron simsii

Cited by 6 publications

References 7 publications

Breeding and selection of Buxus for resistance to Calonectria pseudonaviculata

Breeding and selection of Buxus for resistance to Calonectria pseudonaviculata

Insight into the Molecular Mechanism of Flower Color Regulation in Rhododendron latoucheae Franch: A Multi-Omics Approach

Jasmonic Acid and Salicylic Acid Levels in Defense Response of Azalea (Rhododendron simsii Hybrid) to Broad Mite (Polyphagotarsonemus latus)

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