Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers

Abstract

This study describes the identification of a quantitative trait locus (QTL) in the recombinant inbred line population of ILL2024 × ILL6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes.

Genomic selection for target traits in the Australian lentil breeding program

“…BOR toxicity tolerance was assessed using a hydroponic screening method as described in ( Rodda et al., 2018 )), where six plants per genotype were grown in trays of peat plugs floating in a dilute boric acid solution for two weeks. Lentil varieties previously characterized as intolerant (PBA Blitz and Cassab) and tolerant (ILL2024) were used as internal controls to determine the ideal assessment time.…”

“…These variations arise primarily from the influence of various biotic and environmental stress factors. Of these, ascochyta blight, botrytis grey mould, boron and salinity have been identified as key limiting factors across a wide range of production regions ( Davidson et al., 2016 ; Sudheesh et al., 2016 ; Rodda et al., 2017 ; Rodda et al., 2018 ; Dissanayake et al., 2020 ; Kumar et al., 2021 ) that can result in yield losses of 30-40% ( Kumar et al., 2013 ). Climate variability and complex genotype-by-environment (G × E) interactions on the expression of phenotypic traits also contribute to low genetic gain in lentil breeding ( Kumar and Ali, 2006 ; Kumar et al., 2020 ).…”

Two fingerprinting sets for Humulus lupulus based on KASP and microsatellite markers

“…Yamauchi et al [28] identified four SNP rich regions to genotype 21 cultivated hop varieties from Europe and the United States, and Van Holle et al [45] identified 1,830 highly discriminant SNPs to genotype 56 of the most commercially relevant hop cultivars. KASP markers are starting to gain attention as a valuable genetic tool in plants for genotyping and marker assisted selection (MAS) [124][125][126][127][128][129].…”