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Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover was analysed. We found that the final seed yield mostly depends on the seed number per flower head (SN/FH), which strongly correlated with 1<sup>st</sup> component of PCA during first (r = 0.91) and second (0.92) harvest. Meanwhile, the cluster analysis showed that the typical wild red clover has a lower seed weight than the cultivars and could be clustered on the basis of seed homogeneity. Finally, based on the seed phenotype and harvest components, there were five prospective accessions (2177, 2871, 2876, 2898 and 2899) for a new cultivar prototype.
Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover was analysed. We found that the final seed yield mostly depends on the seed number per flower head (SN/FH), which strongly correlated with 1<sup>st</sup> component of PCA during first (r = 0.91) and second (0.92) harvest. Meanwhile, the cluster analysis showed that the typical wild red clover has a lower seed weight than the cultivars and could be clustered on the basis of seed homogeneity. Finally, based on the seed phenotype and harvest components, there were five prospective accessions (2177, 2871, 2876, 2898 and 2899) for a new cultivar prototype.
The article presents the results of a study in 2021 of 13 tetraploid selective varieties of alsike clover (Trifolium hybridum L.) from Norway, Germany, Sweden, Denmark, Canada, Latvia, Belarus and Russia in terms of the productivity of air-dry matter of the standing crop and seed yield. The observations were carried out in a greenhouse experiment set up in the greenhouse complex of the Federal Williams Research Center of Forage Production & Agroecology. The purpose of the research is to choose for crossing and further breeding work the most productive tetraploid clover genotypes from different geographical regions in terms of standing crop and seed yield. The criterion for selecting promising selective numbers for crossing was the deviation of the standing crop yield and (or) seed yield by more than 3 standard deviations (σ) from the corresponding arithmetic mean in the experiment. For experimental data on the yield of air-dry matter of the standing crop, a normal distribution was characteristic, and for seed yield, an exponential distribution. This made it possible to use the critical value of 3σ in making breeding decisions. The average productivity of air-dry matter of the standing crop is 47 g/vessel, σ=16 g/vessel. The average seed productivity is 1.1 g/vessel, and σ=0.9 g/vessel. According to the value of air-dry matter of the standing crop of 102 g/vessel, selective number 56 (cv. Tetraploid from the Republic of Belarus) was identified, and according to seed yield of 4.2 g/vessel, selective number 42 (cv. Alpo from Norway). They belonged to the productivity of Novator variety 213% and 233%, respectively. Selective numbers 56 and 42 are promising for crossing and studying the ability of offspring to combine high fodder and seed productivity.
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