Breeding of new high-temperature-tolerant strains of Flammulina velutipes

Lee, Kang; Han, Fei; Lin, Jizhen; Guo, Liejin; Wei-fang, Bai

doi:10.1016/j.scienta.2012.12.024

Cited by 19 publications

(5 citation statements)

References 17 publications

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“…Interestingly, the high-temperature-tolerant strain of F. velutipes was successfully generated using EMS, and the mutant strains could be grown with fruiting body formation at high temperatures. Moreover, the BE of the mutants was increased under this condition (Lin-Zhi et al, 2013). Since P. eryngii has been generally cultivated at 18-25°C, these conditions increased the cost of cultivation in tropical countries due to high energy consumption.…”

Section: Discussionmentioning

confidence: 94%

“…EMS and MMS have been successfully used for mutagenesis in various fungi. EMS has been successfully employed to induce the mutagenesis in various mushroom verities such as Volvariella volvacea, Flammulina velutipes, and P. ostreatus (Liu et al, 2011;Lin-Zhi et al, 2013;Sharma & Sharma, 2014;Teimoori et al, 2014) while MMS has been successfully induced mutation in Hypsizygus marmoreus (Lee et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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CHEMICALLY INDUCED MUTAGENESIS IN THE KING OYSTER MUSHROOM Pleurotus eryngii TO GENERATE HIGH-TEMPERATURE TOLERANT STRAIN

Romruen

Thangsiri

Pongsutas

et al. 2021

JEBAS

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In this study, a high-temperature-tolerant strain of the king oyster mushroom (Pleurotus eryngii) was generated by chemical mutagenesis. Cultivation of P. eryngii generally involves incubating the mycelia at 25°C and then moving the spawns for further incubation at 18°C for fruitification. However, in tropical countries, the temperature is a major concern in the production of oyster mushroom where the average temperature is 32°C. In the current study, the mycelia were treated with ethyl methane sulphonate (EMS) or methyl methane sulphonate (MMS) for chemical-induced mutation. Seven mutants (EMS 1, 2, 6, 26, 35, 36, and 38) from EMS mutagenesis exhibited higher growth rates than the wild-type strain at 32°C. However, mutant strains from MMS mutagenesis showed a low growth rate when compared with wild-type. On sawdust substrate, the spawn running conditions for these strains were performed at 32°C, and fruitification occurred at 18°C. The yield and biological efficiency of EMS 36 and 38 mutants were higher than those of the wild-type strain. The activities of cellulase and xylanase of EMS 36 and 38 mutants showed that both these mutants had higher activities than the wild-type strain which may influence mushroom production. Therefore, these EMS 36 and 38 mutants can be cultivated in tropical countries, which could provide a high yield and reduce the cost during spawn running step.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

CHEMICALLY INDUCED MUTAGENESIS IN THE KING OYSTER MUSHROOM Pleurotus eryngii TO GENERATE HIGH-TEMPERATURE TOLERANT STRAIN

Romruen

Thangsiri

Pongsutas

et al. 2021

JEBAS

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“…According to the modified mutation method using LiCl [19] that stimulated base substitution and significantly enhanced the mutation efficiency in combined mutagenesis [19,20], MSSs were incubated with different concentrations of LiCl (0.025, 0.05, 0.075, 0.1, 0.125, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4 and 0.45%) ( w / v ) prepared in sterile PBS and cultured in darkness at 25 °C for 12 h. Lethality assays and antimicrobial tests were performed, and the optimal concentration of LiCl was selected according to lethality and mutant frequency.…”

Section: Methodsmentioning

confidence: 99%

Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi

Qin

Wang

et al. 2019

Molecules

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To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R2 = 0.9514) and in lyophilized mycelium powder (R2 = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R2 = 0.9727) and in lyophilized mycelium powder (R2 = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R2 = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation; these neutral lipids are potential biomarkers of cordycepin biosynthesis.

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“…A linear mycelial growth test was used to measure the average mycelium growth in the cultivation of Lentinula edodes with sunflower seed halls substrate in combination with different ratios of wheat bran, poplar sawdust or oat straw [14]. The recent study on mycelial linear growth was performed by Lin-Zhi et al [15] on Flammulina velutipes. Thus, reducing the time required for the culture cycle is necessary to decrease production costs.…”

Section: Introductionmentioning

confidence: 99%

Growth Performance and Cultivation of Four Oyster Mushroom Species on Sawdust and Rice Bran Substrates

Owaid¹

2014

JBT

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Pleurotus species are progressively becoming important edible mushrooms worldwide and it is of importance in choosing suitable substrates in a given place to grow them. The study provides basic and valuable information on mycelial growth rates and yield efficacy of four different Pleurotus spp. The highest average mycelium growth of 1.29 mm/day followed by 1.17 mm/day was observed in the bags of P. salmoneostramineus and P. ostreatus grey, respectively. Similarly, the highest cumulative growth of 12.90 cm/day and 11.74 cm/day was recorded in the 30 day old bags of P. salmoneostramineus and P. ostreatus grey, respectively. There was a significant increase (P < 0.05) of about three fold in the pin head formation of P. ostreatus grey (9 days) compared to other mushroom species (2-3 days). P. salmoneostramineus showed a vigorous growth in the fruiting body and the harvesting time required was just 5.67 days. It is clear from the result that there is no correlation between the rate of mycelia growth and pin appearance. The yield varied significantly due to the effect of mycelial growth on the substrate. The highest first flush and number of fruiting bodies was achieved in the yellow oyster followed by P. ostreatus grey and P. salmoneostramineus. In conclusion, sawdust and rice bran could be a promising substrate for linear mycelial growth of P. ostreatus species.

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Breeding of new high-temperature-tolerant strains of Flammulina velutipes

Cited by 19 publications

References 17 publications

CHEMICALLY INDUCED MUTAGENESIS IN THE KING OYSTER MUSHROOM Pleurotus eryngii TO GENERATE HIGH-TEMPERATURE TOLERANT STRAIN

CHEMICALLY INDUCED MUTAGENESIS IN THE KING OYSTER MUSHROOM Pleurotus eryngii TO GENERATE HIGH-TEMPERATURE TOLERANT STRAIN

Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi

Growth Performance and Cultivation of Four Oyster Mushroom Species on Sawdust and Rice Bran Substrates

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