Breeding the Thai jasmine rice variety KDML105 for non-age-related broad-spectrum resistance to bacterial blight disease based on combined marker-assisted and phenotypic selection

Win, KyawKyaw; Korinsak, Siripar; Jantaboon, J.; Siangliw, Meechai; Lanceras-Siangliw, J.; Sirithunya, P.; Vanavichit, Apichart; Pantuwan, G.; Jongdee, Boonrat; Sidhiwong, N.; Toojinda, Theerayut

doi:10.1016/j.fcr.2012.09.007

Cited by 25 publications

(13 citation statements)

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“…XA21 has been introgressed into diverse rice varieties and planting of these Xa21 varieties has served as an effective strategy to control outbreaks of bacterial blight disease (Chen et al, 2000;Sundaram et al, 2008;Win et al, 2012). We have discovered recently that Xoo isolates carrying mutations in raxX are able to evade XA21-mediated immunity (Mishra et al, 2013;Pruitt et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Suppression of bacterial infection in rice by treatment with a sulfated peptide

Wei

Chern

Liu

et al. 2016

Molecular Plant Pathology

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The rice XA21 receptor kinase confers robust resistance to bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo). A tyrosine-sulfated peptide from Xoo, called RaxX, triggers XA21-mediated immune responses, including the production of ethylene and reactive oxygen species and the induction of defence gene expression. It has not been tested previously whether these responses confer effective resistance to Xoo. Here, we describe a newly established post-inoculation treatment assay that facilitates investigations into the effect of the sulfated RaxX peptide in planta. In this assay, rice plants were inoculated with a virulent strain of Xoo and then treated with the RaxX peptide 2 days after inoculation. We found that post-inoculation treatment of XA21 plants with the sulfated RaxX peptide suppresses the development of Xoo infection in XA21 rice plants. The treated plants display restricted lesion development and reduced bacterial growth. Our findings demonstrate that exogenous application of sulfated RaxX activates XA21-mediated immunity in planta, and provides a potential strategy for the control of bacterial disease in the field.

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Section: Discussionmentioning

confidence: 99%

Suppression of bacterial infection in rice by treatment with a sulfated peptide

Wei

Chern

Liu

et al. 2016

Molecular Plant Pathology

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“…The number of molecular markers used for genome-wide scanning, which ultimately determines cost vs. precision, has varied from 44 to 205 SSR loci in rice. Four groups have reported rice MAB projects involving background selection: Group 1) used 44–51 SSRs (Yi et al 2009 ; Siangliw et al 2003 ; Tomita 2009 ; Wongsaprom et al 2010 ), Group 2 used 67–72 SSRs (Win et al 2012 ; Singh et al 2013 ), Group 3 used 84–97 SSRs (Siangliw et al 2007 ; Jantaboon et al 2011 ), and Group 4 used 107–205 SSRs (Rajpurohit et al 2011 ; Korinsak et al 2011 ; Suh et al 2013 ). However, the majority of these MAB projects utilized molecular markers during the final stage of selection.…”

Section: Discussionmentioning

confidence: 99%

“…If the ultimate goal is to improve a specific desired variety, parallel backcrossing of single donors can most effectively be carried out using both foreground and background selection. Recently, successful gene/QTL pyramiding programs were reported in Thai Jasmine (Win et al 2012 ; Luo and Yin, 2013 ), Basmati (Singh et al 2012 ; Singh et al 2013 ), Koshihikari (Ashikari and Matsuoka, 2006 ; Tomita, 2009 ), Zhenshan 97 (Wang et al 2012 ) and 93–11 (Zong et al 2012 ) rice varieties. However, when the target traits are quantitatively controlled, combining several QTLs can take years to accomplish.…”

Section: Introductionmentioning

confidence: 99%

Pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety

Ruengphayak

Chaichumpoo

Phromphan

et al. 2015

Rice

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BackgroundPyramiding multiple genes into a desirable genetic background can take years to accomplish. In this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. PinK3, an aromatic and potentially high-yielding rice variety—although one that is intolerant to flash flooding (Sub) and susceptible to bacterial leaf blight (BB), leaf-neck blast (BL) and the brown planthopper (BPH)—was used as a genetic basis for significant improvements through gene pyramiding.ResultsFour resistance donors with five target genes (Sub1A-C, xa5, Xa21, TPS and SSIIa) and three QTLs (qBph3, qBL1 and qBL11) were backcrossed individually using markers into the pseudo-recurrent parent ‘PinK3’ via one cycle of backcrossing followed by two cycles of pseudo-backcrossing and three selfings with rigorous foreground marker-assisted selection. In total, 29 pseudo-backcross inbred lines (BILs) were developed. Genome composition was surveyed using 61 simple sequence repeats (SSRs), 35 of which were located on six carrier chromosomes, with the remainder located on six non-carrier chromosomes. The recurrent genome content (%RGC) and donor genome content (%DGC), which were based on the physical positions of BC1F2, ranged from 69.99 to 88.98% and 11.02 to 30.01%, respectively. For the pseudo-BC3F3BILs, the %RGC and %DGC ranged from 74.50 to 81.30% and 18.70 to 25.50%, respectively. These results indicated that without direct background selection, no further increases in %RGC were obtained during pseudo-backcrossing, whereas rigorous foreground marker-assisted selection tended to reduce linkage drag during pseudo-backcrossing. The evaluation of new traits in selected pseudo-BC3F3BILs indicated significant improvements in resistance to BB, BL, BPH and Sub compared with PinK3, as well as significant improvements in grain yield (21-68%) over the donors, although yield was 7-26% lower than in ‘PinK3’. All pyramided lines were aromatic and exhibited improved starch profiles, rendering them suitable for industrial food applications.ConclusionsResults show that our new pyramiding platform, which is based on marker-assisted pseudo-backcrossing, can fix five target genes and three QTLs into a high-yielding pseudo-recurrent background within seven breeding cycles in four years. This multiple pseudo-backcrossing platform decreases the time required to generate new rice varieties exhibiting complex, durable resistance to biotic and abiotic stresses in backgrounds with desirable qualities.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-014-0035-0) contains supplementary material, which is available to authorized users.

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“…The latter, Xa21, is the most broad-spectrum resistance to Xoo strains, inherited from the wild rice species O. longestaminata. It was widely used in rice breeding programmes (Khush, Mackill, & Sidhu, 1989 To date, several breeding programmes to improve BLB resistance in Thai rice varieties have been developed Win et al, 2012;Pinta, Toojinda, Thummabenjapone, & Sanitchon, 2013). In most cases, researchers utilised the resistance lines from introduced varieties, such as IRBB21, IR24, IRBB5 and IR62266.…”

Section: Resultsmentioning

confidence: 99%

Stability of Four New Sources of Bacterial Leaf Blight Resistance in Thailand Obtained from Indigenous Rice Varieties

et al. 2017

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Bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious diseases in rice production. Breeding varieties specifically for their resistance to BLB disease is therefore an efficient and cost-effective strategy. However, the resistance gene for BLB can be race and non-race specific, meaning it is often overcome by the pathogen. The identification of new sources of resistance genes for Xoo is crucial in rice breeding programmes. In this study, six rice varieties were assessed using six Xoo isolates in multiple screening conditions. The GGE biplot analysis considers both genotype (G) and genotype environment (GE) interaction effects and demonstrates GE interaction. The first two principal components (PCs) accounted for 95.46% of the total GE variation in the data. Based on lesion length and stability performance, Phaladum was the most ideal genotype against all Xoo isolates in the four screening conditions. The results relayed that Phaladum indigenous rice varieties could be considered as new sources of bacterial leaf blight resistance in Thailand. In the future, the BLB resistance gene in this variety will be identified in regard to mode of inheritance and used as parental line in rice breeding programmes for resistance to BLB.

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Breeding the Thai jasmine rice variety KDML105 for non-age-related broad-spectrum resistance to bacterial blight disease based on combined marker-assisted and phenotypic selection

Cited by 25 publications

References 41 publications

Suppression of bacterial infection in rice by treatment with a sulfated peptide

Suppression of bacterial infection in rice by treatment with a sulfated peptide

Pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety

Stability of Four New Sources of Bacterial Leaf Blight Resistance in Thailand Obtained from Indigenous Rice Varieties

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