Bridge-DNA synthesis triggered by an allosteric aptamer for the colorimetric detection of pathogenic bacteria

Abstract

Rapid and sensitive quantification of pathogenic bacteria is highly desired for environmental health supervision and food safety control. Yet, the amplification and detection of bacteria with concentration lower than 102...

Colorimetric Detection and Killing of Bacteria by Enzyme-Instructed Self-Aggregation of Peptide-Modified Gold Nanoparticles

“…According to the calculation, the detection limit was 7 CFU•mL −1 (LOD = 3δ/k). The detection limit of the probe is relatively low compared with other reported bacterial colorimetric detection methods [10,21,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62] (Supplementary Materials Table S2). These results indicate that the AuNPs-CF 4 KY P are selective and sensitive in response to S. typhimurium, and have a low detection limit, which has great application prospects in real life.…”

Rapid and Sensitive Quantification of Bacterial Viability Using Ratiometric Fluorescence Sensing

“…However, the platecounting method is labor-intensive and time-consuming, because a tremendous amount of plating work is necessary and it takes a long time to form visible colonies that can be easily numbered. 4,5 In addition, direct viable counting may be influenced by different culture conditions (e.g., temperature) and cell growth rates, leading to large random errors. 6 In the past two decades, a number of other methods had been developed for differentiating dead bacteria from live/dead cell mixtures, including electron microscopy, 7−9 real-time fluorescent quantitative PCR (qPCR), 10 surface-enhanced Raman scattering, 11 aggregation-induced emission (AIE), 12 and fluorescent techniques combined with fluorescence spectra, 13,14 optical microscopy, 15−17 or flow cytometry (FCM).…”

“…In the method, the single-cell bacteria reproduce on a solid medium and finally form scattered-bacterial colonies, which are visible and numerable to human eyes. However, the plate-counting method is labor-intensive and time-consuming, because a tremendous amount of plating work is necessary and it takes a long time to form visible colonies that can be easily numbered. , In addition, direct viable counting may be influenced by different culture conditions (e.g., temperature) and cell growth rates, leading to large random errors …”