Transportation networks are one of the most vulnerable civil infrastructures during an earthquake and an estimation of traffic impacts in the post-earthquake scenario is a crucial aspect in the context of risk assessment and evaluation of remedial measures. In this paper, a methodology is presented, combining GIS tools, probabilistic seismic risk analysis and traffic simulation models, which is able to assess the direct and indirect (social) costs: bridge repairs, increase in travel time and a lack of accessibility. Operating issues related to the development and calibration of traffic models applicable to a damaged road network are carefully analysed and reviewed and an innovative approach to evaluate the social cost due to the lack of accessibility is also proposed. The developed modelling framework has been applied on a realistic bridge stock within a road transportation network in central Italy where local land-use data have been collected, extensive traffic surveys have been performed and a traffic model has been calibrated. A probabilistic risk analysis employing a ShakeMap derived from a historical real seismic event has been carried out.