Bridging the gap between testing and clinics exploring alternative pre-clinical models in melanoma research

Viegas, Juliana; Sarmento, Bruno

doi:10.1016/j.addr.2024.115295

Cited by 11 publications

(2 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, spheroid and organoid skin models have the drawback of not producing the complex arrangement of skin structures ( Sharma et al, 2023 ). Due to the complex organization of the skin and various complexities associated with the different types of skin cancer, 3D bioprinting with layers offers an attractive alternative to 3D cultures as illustrated in Figure 2 ( Fernandes et al, 2022 ; Shukla et al, 2022 ; Viegas and Sarmento, 2024 ).…”

Section: Skin Cancer Modelsmentioning

confidence: 99%

“…These properties are dependent on age, and representing the age at which a certain skin cancer is predominant is therefore important ( Ansaf et al, 2023 ). 3D Bioprinted skin models have the potential to be used in skin regeneration and wound healing, but also to provide pre-clinical models for drug development or disease modeling ( Pontigga et al, 2022 ; Ansaf et al, 2023 ; Viegas and Sarmento, 2024 ). There are various studies on 3D bioprinted skin models as reviewed by Ansaf et al, 2023 , but there are relatively fewer studies on 3D bioprinted skin cancer models.…”

Section: The Importance Of Viscosity On the Spreading And Migration O...mentioning

confidence: 99%

See 1 more Smart Citation

The influence of viscosity of hydrogels on the spreading and migration of cells in 3D bioprinted skin cancer models

Du Plessis,

Gouws,

Nieto

2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Various in vitro three-dimensional (3D) tissue culture models of human and diseased skin exist. Nevertheless, there is still room for the development and improvement of 3D bioprinted skin cancer models. The need for reproducible bioprinting methods, cell samples, biomaterial inks, and bioinks is becoming increasingly important. The influence of the viscosity of hydrogels on the spreading and migration of most types of cancer cells is well studied. There are however limited studies on the influence of viscosity on the spreading and migration of cells in 3D bioprinted skin cancer models. In this review, we will outline the importance of studying the various types of skin cancers by using 3D cell culture models. We will provide an overview of the advantages and disadvantages of the various 3D bioprinting technologies. We will emphasize how the viscosity of hydrogels relates to the spreading and migration of cancer cells. Lastly, we will give an overview of the specific studies on cell migration and spreading in 3D bioprinted skin cancer models.

show abstract

Section: Skin Cancer Modelsmentioning

confidence: 99%

Section: The Importance Of Viscosity On the Spreading And Migration O...mentioning

confidence: 99%

The influence of viscosity of hydrogels on the spreading and migration of cells in 3D bioprinted skin cancer models

Du Plessis,

Gouws,

Nieto

2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

Targeting the SPC25/RIOK1/MYH9 Axis to Overcome Tumor Stemness and Platinum Resistance in Epithelial Ovarian Cancer

Jiang,

Yang,

Zhang

et al. 2024

Advanced Science

View full text Add to dashboard Cite

In epithelial ovarian cancer (EOC), platinum resistance, potentially mediated by cancer stem cells (CSCs), often leads to relapse and treatment failure. Here, the role of spindle pole body component 25 (SPC25) as a key determinant promoting stemness and platinum resistance in EOC cells, with its expression being correlated with adverse clinical outcomes is delineated. Mechanistically, SPC25 acts as a scaffolding platform, orchestrating the assembly of an SPC25/RIOK1/MYH9 trimeric complex, triggering RIOK1‐mediated phosphorylation of MYH9 at Ser1943. This prompts MYH9 to disengage from the cytoskeleton, augmenting its nuclear accumulation, thus potentiating CTNNB1 transcription and subsequent activation of Wnt/β‐catenin signaling. CBP1, a competitive inhibitory peptide, can disrupt the formation of the aforementioned trimeric complex, diminishing the activity of the SPC25/RIOK1/MYH9 axis–mediated Wnt/β‐catenin signaling, and thus attenuate CSC phenotypes, thereby enhancing platinum efficacy in vitro, in vivo, and in patient‐derived organoids. Therefore, targeting the SPC25/RIOK1/MYH9 axis, which mediates the maintenance of stemness and platinum resistance in EOC cells, may enhance platinum sensitivity and increase survival in patients with EOC.

show abstract

Patient‐Derived Melanoma Immune‐Tumoroids as a Platform for Precise High throughput Drug Screening

Viegas,

Costa,

Dias

et al. 2024

Advanced Science

View full text Add to dashboard Cite

In vitro models are crucial in cancer research, but they must truthfully mimic in vivo tumors for clinical relevance. The development of unprecedent melanoma quadruple multicellular tumoroids (MCTs) is proposed comprising tumor cells, keratinocytes, fibroblasts, and monocytes that replicate tumor architecture, tumor microenvironment, and secretome behavior. These MCTs of 300 µm in diameter secreted keratin and collagen, showing complexity proportional to their cell combinations. Further, closely resembled in vivo tumors in terms of cells organization, growth, progression, and immune behavior. Drug screening using these MCTs demonstrated their potential as patient‐derived platforms for precision medicine. These findings highlight the true value of MCTs for studying melanoma biology and testing therapeutic interventions with greater precision and relevance to human disease.

show abstract

Bridging the gap between testing and clinics exploring alternative pre-clinical models in melanoma research

Cited by 11 publications

References 80 publications

The influence of viscosity of hydrogels on the spreading and migration of cells in 3D bioprinted skin cancer models

The influence of viscosity of hydrogels on the spreading and migration of cells in 3D bioprinted skin cancer models

Targeting the SPC25/RIOK1/MYH9 Axis to Overcome Tumor Stemness and Platinum Resistance in Epithelial Ovarian Cancer

Patient‐Derived Melanoma Immune‐Tumoroids as a Platform for Precise High throughput Drug Screening

Contact Info

Product

Resources

About