Brief fixation does not hamper the reliability of Ki67 analysis in breast cancer core‐needle biopsies: a double‐centre study

Kalkman, Shona; Bulte, J.P.; Halilović, Altuna; Bult, Peter; Diest, Paul J. van

doi:10.1111/his.12521

Cited by 6 publications

(11 citation statements)

References 20 publications

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“…CNBs were placed in a rapid microwave histoprocessor (Pathos, Milestone Srl, Sorisole, Italy) which processes the specimens in approximately 1 hour and 26 min. The detailed protocol of the microwave histoprocessor is described elsewhere 21. Total fixation time for aCNBs varied from approximately 60 up to 90 min.…”

Section: Methodsmentioning

confidence: 99%

“…An extensive description of the immunohistochemical process was previously published 21. Antibody-specific variances are specified: heat-induced antigen retrieval for ER and E-cadherin staining was conducted in 10 mM EDTA (pH 9.0) in a pre-treatment module (Thermo Scientific, Thermo Fisher Scientific, Waltham, Massachusetts, USA) and for PR staining in 10 mM sodium citrate (pH 6.0) in a high-pressure cooker (2100 Retriever, Aptum, Bio-Connect, Huissen, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

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Brief fixation enables same-day breast cancer diagnosis with reliable assessment of hormone receptors, E-cadherin and HER2/Neu

et al. 2017

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Brief fixation enables same-day breast cancer diagnosis with reliable assessment of hormone receptors, E-cadherin and HER2/Neu

et al. 2017

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“…The use of this technique for basic histology was established previously . The reliability for hormone receptor status, E‐cadherin, Ki‐67 and HER2 FISH has also been described …”

Section: Discussionmentioning

confidence: 99%

“…The processing programme started with a 30‐min formaldehyde fixation step under vacuum, leading to a total fixation time of approximately 45 min. The full programme, which has been described previously in greater detail, takes 1 h 39 min …”

Section: Methodsmentioning

confidence: 99%

“…Details of this process have been described previously. 12 In UMC Utrecht, all aCNB specimens were transported in 10% neutral buffered formalin (NBF) and sent to the pathology laboratory to be registered (estimated fixation time approximately 15 min). Specimens were placed subsequently in an automated tissue processor (Peloris TM ; Leica, Valkenswaard, the Netherlands).…”

Section: T I S S U E P R O C E S S I N Gmentioning

confidence: 99%

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Assessment of HER2 status in breast cancer biopsies is not affected by accelerated tissue processing

et al. 2018

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HER2 status can be determined reliably on CNB specimens with accelerated processing time using standard clinical testing methods. Using this accelerated technology the minimum 6 h of formalin fixation, which current guidelines consider necessary, can be decreased safely. This allows for a complete and expedited histology-based diagnosis of breast lesions in the setting of a one-stop-shop, same-day breast clinic.

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Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study

Mahboubi-Ardakani¹,

Dowsett²,

Dowsett³

et al. 2022

Modern Pathology

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Ki67 has potential clinical importance in breast cancer but has yet to see broad acceptance due to inter-laboratory variability. Here we tested an open source and calibrated automated digital image analysis (DIA) platform to: (i) investigate the comparability of Ki67 measurement across corresponding core biopsy and resection specimen cases, and (ii) assess section to section differences in Ki67 scoring. Two sets of 60 previously stained slides containing 30 core-cut biopsy and 30 corresponding resection specimens from 30 estrogen receptor-positive breast cancer patients were sent to 17 participating labs for automated assessment of average Ki67 expression. The blocks were centrally cut and immunohistochemically (IHC) stained for Ki67 (MIB-1 antibody). The QuPath platform was used to evaluate tumoral Ki67 expression. Calibration of the DIA method was performed as in published studies. A guideline for building an automated Ki67 scoring algorithm was sent to participating labs. Very high correlation and no systematic error (p = 0.08) was found between consecutive Ki67 IHC sections. Ki67 scores were higher for core biopsy slides compared to paired whole sections from resections (p ≤ 0.001; median difference: 5.31%). The systematic discrepancy between core biopsy and corresponding whole sections was likely due to pre-analytical factors (tissue handling, fixation). Therefore, Ki67 IHC should be tested on core biopsy samples to best reflect the biological status of the tumor.

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Brief fixation does not hamper the reliability of Ki67 analysis in breast cancer core‐needle biopsies: a double‐centre study

Abstract: Overall agreement for Ki67 expression was good between briefly fixed CNBs and conventionally fixed resection specimens, and within the range of studies comparing conventionally fixed CNBs and resections.

Cited by 6 publications

References 20 publications

Brief fixation enables same-day breast cancer diagnosis with reliable assessment of hormone receptors, E-cadherin and HER2/Neu

Brief fixation enables same-day breast cancer diagnosis with reliable assessment of hormone receptors, E-cadherin and HER2/Neu

Assessment of HER2 status in breast cancer biopsies is not affected by accelerated tissue processing

Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study

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