Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Kaplow, Leonard S.; Ladd, Charlotte O.

doi:10.1182/blood.v26.2.215.215

Cited by 648 publications

(111 citation statements)

References 0 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…was used for assessment of non-specific esterase activity in cells according to the manufacturer's instructions. (iii) Endogenous myeloperoxidase activity was examined using a method described by Kaplow (1965). (iv) Astra blue was used for staining of mast cells as described previously (Strobel et al, 1981).…”

Section: Methodsmentioning

confidence: 99%

Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow

O’Neill

1999

Br J Haematol

View full text Add to dashboard Cite

Summary. Long-term stroma-dependent cultures from murine spleen have been previously shown to support dendritic cell (DC) development in vitro. Secondary cultures have now been established using a splenic stromal cell layer overlaid with cells from different lymphoid sites. Cells resembling DCs can be generated in vitro from unfractionated murine lymphoid cells in the absence of added growth factors. Bone marrow (BM) cultures are the most successful but some cultures have been derived from spleen and thymus. Large numbers of mononuclear cells with dendritic morphology can be generated from BM within 2 weeks and cell production in many cultures has been maintained for at least 6 months. A significant proportion of cells binds antibodies specific for DC markers. No lymphoid cells, mast cells or granulocytes are detectable in culture by antibody and histochemical staining and light and electron microscopy. As with cells generated in primary cultures, cells grown in secondary cultures are equally potent stimulators of both syngeneic and allogeneic mixed lymphocyte reactions, confirming their function as antigen-presenting cells. They are also capable of endocytosing and presenting protein antigen to the D10.G4.1 Th2 clone and to unprimed T cells.This study confirmed the presence of DC precursors in multiple lymphoid sites which can be induced to proliferate in the presence of a spleen stromal cell monolayer. The secondary culture system provides an ideal in vitro model for investigation of the development of DC from different tissue sites. It also provides a stable and continuous resource of cells for further studies on DC development.

show abstract

Section: Methodsmentioning

confidence: 99%

Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow

O’Neill

1999

Br J Haematol

View full text Add to dashboard Cite

show abstract

“…Following 90 min incubation, cells that had ingested yeasts were compared with cells from tubes in which phagocytosis was blocked by thc prcs-ence of 0.006 M EDTA. Slides prepared with the cytocentrifuge were stained for peroxidase (Kaplow, 1965)~ acid phosphatase (Barka & Anderson, 1962) and 8-glucuronidase (Hayashi, 1964). Alkaline phosphatase was scored as described by Dacie & Lewis (1975).…”

Section: Ellzyttle Sttrdiesmentioning

confidence: 99%

Defective Neutrophil Function in Chronic Granulocytic: Leukaemia

El‐Maalem

Fletcher

1976

Br J Haematol

View full text Add to dashboard Cite

The ability of neutrophils to phagocytose, kill and digest Candida guilliermondii was investigated in twelve patients with chronic granulocytic leukaemia (CGL). Following ingestion of organisms there was considerable reduction in the ability of CGL cells to kill Candida and this was not explained by a mild impairment of phagocytosis. Histochemical staining showed that granules containing lysosomal enzymes disappear form the cytoplasm of normal neutrophils during killing and digestion of the fungus, while in CGL cells the granules remain. Quantitative measurement confirmed loss of peroxidase from normal neutrophils during Candida killing but no loss from CGL neutrophils. The primary granules of CGL neutrophils do not fuse with and discharge their contents into the phagocytic vacuole and this explains their impaired ability to kill and digest Candida.

show abstract

“…Cells were stained for non-specific esterase using alpha-naphthyl acetate as substrate [19]. Peroxidase staining was performed using the method of Kaplow [20]. Lysozyme secretion was assayed by reacting 48-h culture supernatants (0.5 ml) with suspensions of Micrococcus lysodeikticus (Sigma, St. Louis, MO) at 0.5 mg/ml in a final volume of 1.0 ml and measuring the time course of lysis of the bacteria photometrically.…”

Section: Enzyme Activitymentioning

confidence: 99%

The characteristics of macrophage-like cell lines derived from normal sheep spleens

Ögmundsdóttir

Hardarson

Steinarsdóttir³

et al. 1991

FEMS Microbiology Letters

View full text Add to dashboard Cite

Macrophage‐like cell lines were derived from sheep spleens using conditioned medium from L‐929 mouse cells as a source of colony stimulating factor. In seven out of ten attempts colonies of macrophage‐like cells appeared after 2–3 weeks of culture. The cells were established in culture as cell lines, and survived 120 passages. They were strongly (++) positive for non‐specific esterase but negative for peroxidase and produced detectable but small amounts of lysozyme (0.21–1.76 μg/106 cells). Latex particles were actively phagocytosed. Bacteria (Staphylococcus albus, Staphylococcus aureus) attached to the cell surface and were internalized in the presence of specific antibody. Expression of receptors for immunoglobulin and complement varied somewhat between the different cell lines; the proportion of receptor‐bearing cells ranged between 9 and 26% FC‐receptors, and 10 and 38% for C‐receptors. The cell lines displayed a peculiar karyotype as well as protein profile that were different from normal sheep but similar between the different cell lines. Culture supernatants of the cell lines contained a colony stimulating activity which was used to establish further cell lines. They also spontaneously produced an interleukin‐1‐like activity that had no effect on baseline proliferation of sheep lymphocytes but enhanced their response to PHA (1.7‐fold) particularly in conjunction with sheep IL‐2 (4‐fold). Prostaglandin E2 was produced in a growth‐cycle dependent manner: the peak production occured on the second day (77–140 pg/ml) at 2 × 105 cells and declined to 33–50 pg/ml on the eighth day when cell numbers had increased to 2–3 × 10106. These easily cultured cell lines derived from normal tissue without the introduction of viral DNA should provide a useful source of material for studies of macrophage function in sheep.

show abstract

Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Cited by 648 publications

References 0 publications

Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow

Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow

Defective Neutrophil Function in Chronic Granulocytic: Leukaemia

The characteristics of macrophage-like cell lines derived from normal sheep spleens

Contact Info

Product

Resources

About