Brief review on the roles of neutrophils in cancer development

Wang, Long; Chen, Jingjing; Chen, Gao; Lin, Zijia; Xie, Xubiao; Dai, Helong

doi:10.1002/jlb.4mr0820-011r

Cited by 24 publications

(15 citation statements)

References 109 publications

(184 reference statements)

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“…Reduced immune recognition, increased tumour cell survival, or development of an immunosuppressive tumour microenvironment (TME) contribute to the tumour escape stage [9]. The TME establishment is a slow process, and recent studies showed that the early-and late-stage TMEs of multiple cancers have cells with different cell heterogeneity and functions [10][11][12][13][14]. Recent advances in multi-omics and single-cell RNA sequencing (scRNA-seq) techniques have contributed significantly to the characterisation of TME, which have resulted in the discovery of more cell types and their response to therapies [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Single-nucleus RNA sequencing and deep tissue proteomics reveal distinct tumour microenvironment in stage-I and II cervical cancer

Liu

Zhang

et al. 2023

J Exp Clin Cancer Res

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Background Cervical cancer (CC) is the 3rd most common cancer in women and the 4th leading cause of deaths in gynaecological malignancies, yet the exact progression of CC is inconclusive, mainly due to the high complexity of the changing tumour microenvironment (TME) at different stages of tumorigenesis. Importantly, a detailed comparative single-nucleus transcriptomic analysis of tumour microenvironment (TME) of CC patients at different stages is lacking. Methods In this study, a total of 42,928 and 29,200 nuclei isolated from the tumour tissues of stage-I and II CC patients and subjected to single-nucleus RNA sequencing (snRNA-seq) analysis. The cell heterogeneity and functions were comparatively investigated using bioinformatic tools. In addition, label-free quantitative mass spectrometry based proteomic analysis was carried out. The proteome profiles of stage-I and II CC patients were compared, and an integrative analysis with the snRNA-seq was performed. Results Compared with the stage-I CC (CCI) patients, the immune response relevant signalling pathways were largely suppressed in various immune cells of the stage-II CC (CCII) patients, yet the signalling associated with cell and tissue development was enriched, as well as metabolism for energy production suggested by the upregulation of genes associated with mitochondria. This was consistent with the quantitative proteomic analysis that showed the dominance of proteins promoting cell growth and intercellular matrix development in the TME of CCII group. The interferon-α and γ responses appeared the most activated pathways in many cell populations of the CCI patients. Several collagens, such as COL12A1, COL5A1, COL4A1 and COL4A2, were found significantly upregulated in the CCII group, suggesting their roles in diagnosing CC progression. A novel transcript AC244205.1 was detected as the most upregulated gene in CCII patients, and its possible mechanistic role in CC may be investigated further. Conclusions Our study provides important resources for decoding the progression of CC and set the foundation for developing novel approaches for diagnosing CC and tackling the immunosuppressive TME.

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Section: Introductionmentioning

confidence: 99%

Single-nucleus RNA sequencing and deep tissue proteomics reveal distinct tumour microenvironment in stage-I and II cervical cancer

Liu

Zhang

et al. 2023

J Exp Clin Cancer Res

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“…In addition, studies have also demonstrated the prognostic value of NLR on mRCC patients after surgery (Cetin et al, 2013;Lecot et al, 2019). As in ammatory immune cells, neutrophils alter the tumor microenvironment by expressing chemokine receptors CXCR1 and CXCR2 (Long et al, 2021). Moreover, neutrophils also release reactive oxygen species (ROS) and proteases to promote cancer development (Antonio et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value and Model Construction of Preoperative Inflammatory Markers in Patients with Metastatic Renal Cell Carcinoma

Wang

Zhao

et al. 2023

Preprint

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Purpose: Inflammation is considered to be one of the driving factors of cancer, and chronic inflammation plays a crucial role in tumor growth and metastasis. In this study, the aim was to study the predictive value of preoperative inflammatory biomarkers including preoperative neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), and aspartate aminotransferase-to-lymphocyte ratio (ALR) which was a novel inflammatory biomarker, for overall survival (OS) in patients with metastatic renal cell carcinoma (mRCC). Method: This study included a total of 198 patients with mRCC from a single center from 2006 to 2022. The optimal cut-off levels of the three biomarkers were calculated by using the receiver operating characteristic curve (ROC). The patients were divided into two groups (high and low values) according to the cut-off level, and the difference between them was analyzed and compared by the Kaplan-Meier curve. Cox univariate and multivariate analyzes were used to assess independent prognostic inflammatory biomarkers. Finally, independent prognostic inflammatory biomarkers were incorporated into the prognostic model to establish a nomogram to predict the postoperative survival of patients with mRCC. Result: The optimal cut-off level of NLR evaluated by the ROC curve was 3.836, LMR was 3.106, and ALR was 68.056. Patients with NLR and ALR higher than the cut-off level and LMR lower than the cut-off level were significantly related to OS. Multivariate analysis showed that lower LMR and higher ALR were independent risk factors for OS. In addition, a nomogram can accurately predict the OS in patients with mRCC. Conclusion：ALR and LMR are independent risk factors for the prognosis of patients with mRCC. By monitoring ALR and LMR after operation, the prognosis of patients with mRCC can be better evaluated.

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“…Recent studies also pointed out the involvement of tumorassociated neutrophils (TAN) and tumor-associated macrophages (TAM) in maintaining the inflammatory or immunosuppressive TME that dictates the effect of therapies. [217][218][219][279][280][281][282][283][284][285][286] Neutrophils are the most abundant polymorph nuclear (PMN) cells available in the peripheral blood and early accumulated tumorassociated cells following therapies that make the inflammatory milieu. 280,[286][287][288][289][290] However, based on the tumor cell-secreted cy- including cell adhesion, directed migration, granule release, and superoxide production.…”

Section: Engineered Exosomes To Target Neutrophilsmentioning

confidence: 99%

“…[217][218][219][279][280][281][282][283][284][285][286] Neutrophils are the most abundant polymorph nuclear (PMN) cells available in the peripheral blood and early accumulated tumorassociated cells following therapies that make the inflammatory milieu. 280,[286][287][288][289][290] However, based on the tumor cell-secreted cy- including cell adhesion, directed migration, granule release, and superoxide production. 291 Although there are a few ligands that are an agonist for FPRs, we cannot utilize those for targeting neutrophils because they may stimulate the neutrophils for hyperfunctioning.…”

Section: Engineered Exosomes To Target Neutrophilsmentioning

confidence: 99%

Engineered exosomes for studies in tumor immunology

Alptekin

Parvin

Chowdhury

et al. 2022

Immunological Reviews

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Exosomes are a type of extracellular vesicle (EV) with diameters of 30-150 nm secreted by most of the cells into the extracellular spaces and can alter the microenvironment through cell-to-cell interactions by fusion with the plasma membrane and subsequent endocytosis and release of their cargo. [1][2][3][4][5][6] Irrespective of the origin of parent cells, exosomes share common features such as certain tetraspanins (CD9, CD63, and CD81), heat shock proteins (HSP 60, Hsp 70, and Hsp 90), biogenesis-related proteins (Alix and TSG 101), membrane transport and fusion proteins (GTPases, annexins, and Rab proteins), nucleic acids (mRNA, miRNA, and long noncoding RNAs and DNAs), and lipids (cholesterol and ceramide). 2,7,8 Because of their biocompatibility, low toxicity and immunogenicity, permeability (even through the blood-brain barrier (BBB)), stability in biological fluids, and ability to accumulate in the lesions with higher specificity, 9-15 investigators have started making designer's exosomes or engineered exosomes to carry biologically active protein on the surface or inside the exosomes as well as using exosomes to carry drugs, micro RNA, and other products to the site of interest. 11,[16][17][18][19]

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Brief review on the roles of neutrophils in cancer development

Cited by 24 publications

References 109 publications

Single-nucleus RNA sequencing and deep tissue proteomics reveal distinct tumour microenvironment in stage-I and II cervical cancer

Single-nucleus RNA sequencing and deep tissue proteomics reveal distinct tumour microenvironment in stage-I and II cervical cancer

Prognostic Value and Model Construction of Preoperative Inflammatory Markers in Patients with Metastatic Renal Cell Carcinoma

Engineered exosomes for studies in tumor immunology

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