2022
DOI: 10.1111/ejn.15684
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Brief sensory deprivation triggers plasticity of dopamine‐synthesising enzyme expression in genetically labelled olfactory bulb dopaminergic neurons

Abstract: In the glomerular layer of the olfactory bulb, local dopaminergic interneurons play a key role in regulating the flow of sensory information from nose to cortex. These dual dopamine‐ and GABA‐releasing cells are capable of marked experience‐dependent changes in the expression of neurotransmitter‐synthesising enzymes, including tyrosine hydroxylase (TH). However, such plasticity has most commonly been studied in cell populations identified by their expression of the enzyme being studied and after long periods o… Show more

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Cited by 11 publications
(9 citation statements)
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References 91 publications
(204 reference statements)
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“…1C), and the colocalization in dorsal slices was particularly variable (Fig.1D, Chi-squared test, p=0.02). In the OB, in line with previous reports (Galliano et al 2018; Byrne et al 2022) only around 70% of cells expressed both the genetically-encoded DAT-tomato label and TH (double positive 72.17 ± 3.39%, DsRed+/TH-11.7 ± 1.50%, DsRed-/TH+ 16.05 ± 2.35%, n=2652, N=4; Fig. 1C).…”
Section: Resultssupporting
confidence: 92%
See 1 more Smart Citation
“…1C), and the colocalization in dorsal slices was particularly variable (Fig.1D, Chi-squared test, p=0.02). In the OB, in line with previous reports (Galliano et al 2018; Byrne et al 2022) only around 70% of cells expressed both the genetically-encoded DAT-tomato label and TH (double positive 72.17 ± 3.39%, DsRed+/TH-11.7 ± 1.50%, DsRed-/TH+ 16.05 ± 2.35%, n=2652, N=4; Fig. 1C).…”
Section: Resultssupporting
confidence: 92%
“…Given that, to the best of our knowledge, this is the first study to use the DAT-Cre line to electrophysiologically profile midbrain DA neurons, we first characterized this reporter line against the gold standard of TH expression. Previous work showed incomplete penetrance and imperfect specificity in the OB (Galliano et al 2018; Byrne et al 2022), a finding that we replicated: 16% of TH positive neurons failed to express DAT, and 12% of DAT-expressing neurons did not express TH (a population that has been previously characterized as calretinin-positive and whose electrophysiological signatures clearly distinguishes them from DA cells (Fogli Iseppe, Pignatelli, and Belluzzi 2016; Byrne et al 2022)). Conversely, in SNC and VTA over 90% of DAT-tdTomato neurons co-stained for anti-TH antibodies, indicating that the DAT-Cre line captures most midbrain DA neurons with minimal leakage.…”
Section: Discussionsupporting
confidence: 75%
“…In the VTA, the expression patterns were more heterogeneous overall (double positive 85.40 ± 5.32%%, DsRed + /TH − 8.75 ± 4.43%, DsRed − /TH + 6.00 ± 2.35%, n = 752, N = 4; Figure 1C), and the colocalisation in dorsal slices was particularly variable (Figure 1D, χ 2 test, p = 0.02). In the OB, in line with previous reports (Byrne et al, 2022; Galliano et al, 2018), only around 70% of cells expressed both the genetically encoded DAT‐tomato label and TH (double positive 72.17 ± 3.39%, DsRed + /TH − 11.7 ± 1.50%, DsRed − /TH + 16.05 ± 2.35%, n = 2652, N = 4; Figure 1C). The penetrance of the genetic label was equally incomplete across the dorso‐ventral axis (Figure 1D, χ 2 test, p = 0.77) and the rostro‐caudal axis (χ 2 test, p = 0.91).…”
Section: Resultssupporting
confidence: 91%
“…Given that, to the best of our knowledge, this is the first study to use the DAT‐Cre line to electrophysiologically profile midbrain DA neurons, we first characterised this reporter line against the gold standard of TH expression. Previous work showed incomplete penetrance and imperfect specificity in the OB (Byrne et al, 2022; Galliano et al, 2018), a finding that we replicated: 16% of TH positive neurons failed to express DAT, and 12% of DAT‐expressing neurons did not express TH (a population that has been previously characterised as calretinin‐positive and whose electrophysiological signatures clearly distinguishes them from DA cells (Byrne et al, 2022; Fogli Iseppe et al, 2016)). Conversely, in SNC and VTA, over 90% of DAT‐tdTomato neurons co‐stained for anti‐TH antibodies, indicating that the DAT‐Cre line captures most midbrain DA neurons with minimal leakage.…”
Section: Discussionmentioning
confidence: 93%
“…(B) Effect size of olfactory deprivation duration on the TH staining intensity in bulbar DA neurons. Note that 2/3 datapoints originated from the same study(120).…”
Section: Resultsmentioning
confidence: 99%