Bright CD38 expression is an indicator of<i>MYC</i>rearrangement

Maleki, Atousa; Seegmiller, Adam C.; Uddin, Naseem; Karandikar, Nitin J.; Chen, Weina

doi:10.1080/10428190902930470

Cited by 24 publications

(13 citation statements)

References 7 publications

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“…CD38 was positive in all cases by flow cytometry with a mean MFI of 2009 (median 1372, range 271-7931), significantly higher than diffuse large B-cell lymphoma without MYC rearrangement (mean MFI of 880 in our previous study 20 ). Twenty-six cases (76%) had a MFI higher than 1141, which is considered an indicator for MYC rearrangement with a moderately high sensitivity (75%) and specificity (81%).…”

Section: Immunophenotypic Characteristics By Flow Cytometrymentioning

confidence: 42%

“…Consistent with our previous study, the majority of cases in this cohort expressed bright CD38 with an MFI higher than 1141, the threshold that is considered an indicator for MYC rearrangement with a moderately high sensitivity and specificity. 20 Importantly, such bright CD38 expression is consistently present in group II MYC-complex lymphomas (87%), indicating that bright CD38 expression may be a better predictor of MYC rearrangement in this group.…”

Section: Discussionmentioning

confidence: 92%

“…Twenty-six cases (76%) had a MFI higher than 1141, which is considered an indicator for MYC rearrangement with a moderately high sensitivity (75%) and specificity (81%). 20 A representative flow cytometry plot from case 13 is presented in Figure 2.…”

Section: Immunophenotypic Characteristics By Flow Cytometrymentioning

confidence: 99%

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Simple karyotype and bcl-6 expression predict a diagnosis of Burkitt lymphoma and better survival in IG-MYC rearranged high-grade B-cell lymphomas

et al. 2010

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Rearrangement of MYC with immunoglobulin genes is a hallmark of Burkitt lymphoma. However, this rearrangement is not entirely specific and is often accompanied by varying numbers of additional cytogenetic abnormalities. This study aimed to assess the impact of karyotypic complexity, in correlation with comprehensive immunophenotypic analyses on the diagnosis and clinical outcomes of 34 cases of MYC-IG rearranged lymphomas that included Burkitt lymphoma (twenty-two cases), diffuse large B-cell lymphoma (three cases), unclassifiable B-cell lymphoma with features intermediate between diffuse large B-cell lymphoma and Burkitt lymphoma (six cases), and plasmablastic lymphoma (three cases). Additional cytogenetic abnormalities were observed in 26 of 34 cases (76%), including four cases (12%) that harbored dual translocations involving BCL-2 or BCl-6. Burkitt lymphoma cases had a significantly lower number of additional abnormalities (mean of 1.7), compared with unclassified B-cell lymphoma (3.3), diffuse large B-cell lymphoma (21.7), and plasmablastic lymphoma (6.7). Cases with simple karyotype (p2 additional abnormalities) were more likely to have a diagnosis of Burkitt lymphoma (89 versus 33% in patients with 42 additional abnormalities, Po0.01) and express bcl-6 (95 versus 47%, Po0.01). In addition, Burkitt lymphoma, bcl-6 expression, and simple karyotype were individual predictors of better overall survival. However, in multivariate analyses, only bcl-6 expression remained an independent predictor, although survival could be further stratified by karyotypic complexity in bcl-6( þ ) patients. We conclude that simple karyotype and bcl-6 expression suggest a diagnosis of Burkitt lymphoma and may portend better overall survival. These results may be very useful in the diagnosis and stratification of MYC-IG rearranged high-grade B-cell lymphomas.

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Section: Immunophenotypic Characteristics By Flow Cytometrymentioning

confidence: 42%

Section: Discussionmentioning

confidence: 92%

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Simple karyotype and bcl-6 expression predict a diagnosis of Burkitt lymphoma and better survival in IG-MYC rearranged high-grade B-cell lymphomas

et al. 2010

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“…Recent data have suggested that a significant proportion of mature B-cell lymphomas with c-MYC translocations may show a common immunophenotype, including increased expression of CD38, as assessed by immunohistochemical analysis 9 or flow cytometry. 10 As such, we sought to determine whether there might be a common immunophenotype of dual-hit, aggressive B-cell lymphomas that might identify these cases for cytogenetic analysis because this testing might not otherwise be routinely performed. Herein, we report on 10 cases from our cohort and describe their common immunophenotype by multicolor flow cytometry performed at the University of Washington Medical Center (UWMC), Seattle.…”

Section: Lymphomas With 2 Translocations Involving C-myc and Bcl2 Or mentioning

confidence: 99%

“…For the study, 100-μL aliquots of whole blood, whole bone marrow, or disaggregated tissue were stained and processed with a standard whole blood lysing procedure using ammonium chloride containing 0.25% ultrapure formaldehyde following 3 washes with phosphatebuffered saline-bovine serum albumin to remove plasma protein, as previously described. 10 Data were analyzed using in-house software developed by B.L. Wood, University of Washington (software version 2.7.6).…”

Section: Flow Cytometrymentioning

confidence: 99%

“Double-Hit” Mature B-Cell Lymphomas Show a Common Immunophenotype by Flow Cytometry That Includes Decreased CD20 Expression

Wood

Dorer

et al. 2010

American Journal of Clinical Pathology

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Lymphomas with 2 translocations involving c-MYC and BCL2 or BCL6 are a subset of biologically aggressive mature B-cell lymphomas now frequently categorized under the entity of B-cell lymphoma, unclassifiable, with features intermediate between Burkitt lymphoma and diffuse large B-cell lymphoma. We identified a cohort of these lymphomas in our databases and retrospectively reviewed corresponding in-house flow cytometric data to determine whether a common immunophenotype might be present. Herein we report on our findings on 10 lymphomas, each with translocations involving c-MYC and BCL2 or BCL6 and show that these cases frequently showed a common immunophenotype that includes decreased expression of CD20. Because these lymphomas often show aggressive biologic behavior and poor clinical outcome, recognition of this relatively common immunophenotype may be useful for identifying cases for confirmatory cytogenetic studies, as often, flow cytometry provides the first assessment of these clinical specimens.

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2021

Flow Cytometry of Hematological Malignancies

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Bright CD38 expression is an indicator ofMYCrearrangement

Cited by 24 publications

References 7 publications

Simple karyotype and bcl-6 expression predict a diagnosis of Burkitt lymphoma and better survival in IG-MYC rearranged high-grade B-cell lymphomas

Simple karyotype and bcl-6 expression predict a diagnosis of Burkitt lymphoma and better survival in IG-MYC rearranged high-grade B-cell lymphomas

“Double-Hit” Mature B-Cell Lymphomas Show a Common Immunophenotype by Flow Cytometry That Includes Decreased CD20 Expression

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