Brillouin light scattering in biological systems

Kabakova, Irina V.; Scarcelli, Giuliano; Yun, Seok Hyun

doi:10.1016/bs.semsem.2022.05.008

Cited by 3 publications

(2 citation statements)

References 117 publications

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“…Brillouin light scattering is the inelastic scattering of an incident optical wave field by thermally excited elastic waves in a material [ 15 , 16 ]. The technique has been widely used in biology [ 17 ], chemistry [ 18 ], earth science [ 19 ], materials science [ 20 ], and polymer science [ 10 ]. In Brillouin scattering, incident light induces dynamic fluctuations in the strain field to bring about fluctuations in the dielectric constant; these in turn translate into fluctuations in the refractive index due to elasto-optic scattering.…”

Section: Experimental Methodsmentioning

confidence: 99%

Sound Velocity and Equation of State of Ballistic Gelatin by Brillouin Scattering

Ahart

Hemley

2023

Materials

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Brillouin scattering spectroscopy with diamond anvil cells was used by measuring the pressure dependence of the sound-relevant polymer material, glass-forming liquid, and H2O (water and ice VII) velocities of the material from ambient pressure to 12 GPa at room temperature. Measurements of 20%, 10%, and 4% gelatin solutions were performed. For comparison purposes, we also measured the pressure dependence of the sound velocity of animal tissue up to 10 GPa. We analyzed the Brillouin data using the Tait and Vinet equations of state. We discussed the possible influence of frequency dispersion on bulk modulus at low pressure. We compared the elastic moduli obtained for gelatin to those of several other polymers.

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Section: Experimental Methodsmentioning

confidence: 99%

Sound Velocity and Equation of State of Ballistic Gelatin by Brillouin Scattering

Ahart

Hemley

2023

Materials

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“…This technique is based on the interaction of light with the acoustic waves that spontaneously propagate inside the sample to extract information about their mechanical properties: the viscoelastic characterization occurs without any physical contact or labeling, unlike other commonly used approaches [2][3][4][5][6]. This peculiar characteristic made Brillouin spectroscopy a valuable technique to measure the mechanical properties of biological and biomedical samples [7][8][9][10][11][12]. However, the complex and heterogeneous nature of biological samples introduced new challenges on the interpretation of Brillouin spectra, where the mechanical information is highly intertwined with the morphology of the sample [13,14].…”

Section: Introductionmentioning

confidence: 99%

Brillouin spectroscopy for accurate assessment of morphological and mechanical characteristics in micro-structured samples

Passeri,

Argentati,

Morena

et al. 2024

J. Phys. Photonics

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Brillouin spectroscopy has recently attracted attention as a powerful tool for the characterisation of the mechanical properties of heterogeneous materials, particularly in the biological and biomedical domains. This study investigates the procedure to use Brillouin data to provide relevant morphological parameters of micro-structured samples. When acquiring Brillouin spectra at the interface between two regions of the sample, the spectrum shows signatures of both regions. This feature can be used to precisely identify the position of the interfaces by analysing the evolution of the fitting parameters of the Brillouin spectra acquired by performing a linear scan across the interface. This concept has been demonstrated measuring the thickness of adherent HEK 293T cells. The results are validated using fluorescence microscopy, showing an excellent agreement. The present analysis showcases the wealth of information present in the Brillouin spectrum and the potentiality of Brillouin spectroscopy not only for mechanical characterization but also for label-free, high-resolution imaging of sample morphology. The study introduces the possibility of correlating mechanical properties and shape of biological samples using a single technique.

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