Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue

Elsayad, Kareem; Palombo, Francesca; Dehoux, Thomas; Fioretto, D.

doi:10.1364/boe.10.002670

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…To address this point, we used Brillouin light scattering microscopy, a non-invasive technique which has been recently applied to a broad range of living biological systems including different areas of the CNS ( Girard et al, 2015 ; Schlüßler et al, 2018 , Scarcelli and Yun, 2012 ; Scarcelli et al, 2011 ; Mattana et al, 2017 ). This technique provides information about tissue compressibility by measuring the Brillouin shift values of the sample ( Zhang et al, 2017 ; Prevedel et al, 2019 ; Elsayad et al, 2019b , Elsayad et al, 2019a ).…”

Section: Resultsmentioning

confidence: 99%

Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Amini

Bhatnagar

Schlüßler

et al. 2022

eLife

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Migration of cells in the developing brain is integral for the establishment of neural circuits and function of the central nervous system. While migration modes during which neurons employ predetermined directional guidance of either preexisting neuronal processes or underlying cells have been well explored, less is known about how cells featuring multipolar morphology migrate in the dense environment of the developing brain. To address this, we here investigated multipolar migration of horizontal cells in the zebrafish retina. We found that these cells feature several hallmarks of amoeboid-like migration that enable them to tailor their movements to the spatial constraints of the crowded retina. These hallmarks include cell and nuclear shape changes, as well as persistent rearward polarization of stable F-actin. Interference with the organization of the developing retina by changing nuclear properties or overall tissue architecture, hampers efficient horizontal cell migration and layer formation showing that cell-tissue interplay is crucial for this process. In view of the high proportion of multipolar migration phenomena observed in brain development, the here uncovered ameboid-like migration mode might be conserved in other areas of the developing nervous system.

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Section: Resultsmentioning

confidence: 99%

Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Amini

Bhatnagar

Schlüßler

et al. 2022

eLife

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show abstract

“…To address this point, we used Brillouin light scattering microscopy, a non-invasive technique which has been recently applied to a broad range of living biological systems including different areas of the CNS (Girard et al 2015, Schlussler et al 2018, Scarcelli and Yun 2012, Scarcelli, Kim and Yun 2011, Mattana et al 2017. This technique provides information about tissue compressibility by measuring the Brillouin shift values of the sample (Zhang et al 2017, Prevedel et al 2019, Elsayad, Polakova and Gregan 2019b, Elsayad et al 2019a).…”

Section: Dominated By Ecmmentioning

confidence: 99%

Amoeboid-like neuronal migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Amini

Schlüßler

Möllmert

et al. 2021

Preprint

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As neurons are often born at positions different than where they ultimately function, neuronal migration is key to ensure successful nervous system development. Radial migration during which neurons featuring unipolar and bipolar morphology, employ pre-existing processes or underlying cells for directional guidance, is the most well explored neuronal migration mode. However, how neurons that display multipolar morphology, without such processes, move through highly crowded tissue environments towards their final positions remains elusive. To understand this, we here investigated multipolar migration of horizontal cells in the zebrafish retina. We found that horizontal cells tailor their movements to the environmental spatial constraints of the crowded retina, by featuring several characteristics of amoeboid migration. These include cell and nucleus shape changes, and persistent rearward polarization of stable F-actin, which enable horizontal cells to successfully move through the crowded retina. Interference with the organization of the developing retina by changing nuclear properties or overall tissue architecture, hampers efficient horizontal cell migration and layer formation. Thus, cell-tissue interplay is crucial for efficient migration of horizontal cells in the retina. In view of high proportion of multipolar neurons, the here uncovered ameboid-like neuronal migration mode might also be crucial in other areas of the developing brain.

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“…This breakthrough has fuelled the growing demand for BLS hyperspectral viscoelastic imaging of biomedical samples. In fact, BLS imaging can accelerate research in areas such as biomechanics [14,15], mechanobiology [16,17], and tissue engineering [18,19] by providing access to valuable information about the biomechanical properties of cells and tissues [20][21][22]. It is evident that enhancing the reliability of spectral interpretation can significantly expand the clinical utility of BLS imaging, paving the way for innovative diagnostic tools and for evaluating tissue health and disease [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Brillouin scattering from biomedical samples: the challenge of heterogeneity

Cardinali,

Caponi,

Mattarelli

et al. 2024

J. Phys. Photonics

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Brillouin light scattering (BLS), a non-destructive and non-contact technique, offers a powerful tool for probing the micromechanical properties of biological tissues. However, the inherent heterogeneity of biological tissues can pose significant challenges in interpretingBLS spectra. In this study, we introduce a novel method that harnesses the intensity information within a single BLS spectrum to directly estimate the Voigt average of the longitudinal modulus. Additionally, we use a method to determine the ratio of the squaredPockels coefficients for photoelastically heterogeneous samples, based on global analysis of a2D BLS map. This method is shown to effectively determine the photoelastic ratio of soft and hard components of human bone tissues, enabling the calculation of the average elastic constants. Furthermore, it has the remarkable ability to generate maps of the filling factor of the scattering volume, shedding valuable light on the intricate structure and topography of rough surfaces under BLS mapping.

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Brillouin Light Scattering Microspectroscopy for Biomedical Research and Applications: introduction to feature issue

Cited by 12 publications

References 15 publications

Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Amoeboid-like neuronal migration ensures correct horizontal cell layer formation in the developing vertebrate retina

Brillouin scattering from biomedical samples: the challenge of heterogeneity

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