Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents

Abstract

A method, utilizing brine shrimp (Artemia salina Leach), is proposed as a simple bioassay for natural product research. The procedure determines LC (50) values in microg/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool.

Synthesis, spectral characterization, in-vitro microbiological evaluation and cytotoxic activities of novel macrocyclic bis hydrazone

“…It has been suggested that the Schiff base with nitrogen and oxygen donor systems inhibit enzyme activity, since the enzymes which require these groups of their activity appear to be especially more susceptible to deactivation by metal ions on coordination. Moreover, coordination reduces the polarity [15,16] of the metal ion mainly because of the partial sharing of its positive charge with the donor groups [17,18] within the chelate ring system formed during coordination. This process, in turn, increases the lipophilic nature of the central metal atom, which favors its permeation more efficiently through the lipid layer of the microorganism [19][20][21] thus destroying them more aggressively.…”

“…The synthesized Schiff base and its Co(II), Ni(II) and Cu(II) complexes were screened for their cytotoxicity (brine shrimp bioassay) by using the protocol of Meyer et al [17]. Brine shrimp (Artemia salina leach) eggs were hatched in a shallow rectangular plastic dish (22 Â 32 cm) filled with artificial seawater, which was prepared with a commercial salt mixture and double distilled water.…”

In vivo toxicity study of Lantana camara

“…From a pharmacological point, a good relationship has been found with the brine shrimp lethality test to detect antitumoral compounds in terrestrial plant extracts [5,9,10] . Then, root extract as the most toxic part of L. camara is more potential to anticancer phytochemicals.…”

“…Bioactivity of the L. camara extracts was measured by the brine shrimp lethality test [5] . Samples were dissolved in DMSO and diluted with artificial seawater (concentration 100 毺 g/mL).…”

Antioxidant, antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

“…Brine shrimp lethality bioassay was carried out according to Meyer et al [24] to investigate the cytotoxicity of the extract. The dried extract preparation was redissolved in DMSO to obtain a solution of 10 mg/mL of the extract for toxicity test.…”