Bringing microbial interactions to light using imaging mass spectrometry

Shih, Chao-Jen; Chen, Pi-Yu; Liaw, Chih‐Chuang; Lai, Ying-Mi; Yang, Yu‐Liang

doi:10.1039/c3np70091g

Cited by 50 publications

(57 citation statements)

References 111 publications

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“…Advanced analytical imaging techniques, including matrix-assisted laser desorption/ionization highresolution mass spectrometry (MALDI-HRMS) imaging, combined with modern screening and hyphenated separation techniques including HPLC-HRMS n , trace GC-HRMS, and HPLC-NMR, to name a few, can be used to visualize the distribution, localization, and dynamics of target compounds and their precursors, thereby revealing valuable information about the exact site(s) of biosynthesis [29][30][31]. Such approaches are used to provide a holistic understanding of the distribution, biosynthesis, transport, and storage of target and/or selected non-target compounds with limited sample preparation [32].…”

Section: Introductionmentioning

confidence: 99%

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

et al. 2015

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Advanced analytical imaging techniques, including matrix-assisted laser desorption/ionization high-resolution mass spectrometry (MALDI-HRMS) imaging, can be used to visualize the distribution, localization, and dynamics of target compounds and their precursors with limited sample preparation. Herein we report an application of MALDI-HRMS imaging to map, in high spatial resolution, the accumulation of the medicinally important naphthodianthrone hypericin, its structural analogues and proposed precursors, and other crucial phytochemical constituents in the leaves of two hypericin-containing species, Hypericum perforatum and Hypericum olympicum. We also investigated Hypericum patulum, which does not contain hypericin or its protoforms. We focused on both the secretory (dark glands, translucent glands, secretory canals, laminar glands, and ventral glands) and the surrounding non-secretory tissues to clarify the site of biosynthesis and localization of hypericin, its possible precursors, and patterns of localization of other related compounds concomitant to the presence or absence of hypericin. Hypericin, pseudohypericin, and protohypericin accumulate in the dark glands. However, the precursor emodin not only accumulates in the dark glands but is also present outside the glands in both hypericin-containing species. In hypericin-lacking H. patulum, however, emodin typically accumulates only in the glands, thereby providing evidence that hypericin is possibly biosynthesized outside the dark glands and thereafter stored in them. The distribution and localization of related compounds were also evaluated and are discussed concomitant to the occurrence of hypericin. Our study provides the basis for further detailed investigation of hypericin biosynthesis by gene discovery and expression studies.

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Section: Introductionmentioning

confidence: 99%

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

et al. 2015

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“…Note also that the ions of m/z 619.1 (Figure 10b), 1643.6 (Figure 10f) and 1971.5 (Figure 10g) are in greater abundance between colonies and, therefore, related to "cross talking". 14,15,22 The ion of m/z 638.5 (Figure 10c) is, however, in greater abundance only on the colonies of the N. harveyana PCC 7804. The ion of m/z 989.4 (Figure 10d) is detected in all the three colonies, whereas that of m/z 1276.3 (Figure 10e) is abundant except for the colonies of the M. aeruginosa PCC 7820.…”

Section: Resultsmentioning

confidence: 99%

“…MALDI-IMS combines the high sensitivity and selectivity of MS with 2D surface screening analysis, and its scope has been expanded to deliver spatial distributions of several biomolecules such as metabolites and lipids in different biological matrices. 13 This technique has also been applied for analysis of microbial natural products, 14 microbial interactions 15 and metabolic profiling of microorganisms. 16 Recently, MALDI-IMS has been tested for filamentous marine cyanobacteria, 17 showing the spatial distribution of natural products.…”

Section: Maldi Imaging Mass Spectrometry Of Fresh Water Cyanobacteriamentioning

confidence: 99%

MALDI Imaging Mass Spectrometry of Fresh Water Cyanobacteria: Spatial Distribution of Toxins and Other Metabolites

Sandonato¹,

Santos²,

Luizete³

et al. 2016

Journal of the Brazilian Chemical Society

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Cyanobacteria are among the most ancient forms of life, yet they are known to synthesize highly sophisticated defense molecules, such as the highly hepatotoxic cyclic peptides microcystins and nodularins produced by the genera Microcystis, Anabaena and Nodularia. These metabolites are released by cyanobacteria to water environments causing episodes of fatalities among animals and humans. To better understand the releasing of these metabolites, imaging mass spectrometry (IMS) using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) was herein applied to determine the spatial distribution of such toxins directly on agar-based cultures. Other key metabolites such as aeruginosin 602 and the siderophore anachelin were also mapped in mixed cyanobacterial cultures, showing the great potential of IMS to spatially monitor the biochemical details of cyanobacterial defense and interactions. Keywords: cyanobacteria, MALDI-TOF, mass spectrometry, imaging IntroductionCyanobacteria are believed to represent one of the most ancient yet highly sophisticated forms of life 1 and these gram-negative oxygenic photosynthetic autotrophic microorganisms are adapted to various ecological habitats such as fresh water, marine, brackish, glaciers, and terrestrial environments.2 Fresh and brackish water cyanobacteria are known to produce a diversity of highly elaborated secondary metabolites, with a truly fascinating variety of structures that exhibit a broad range of applications in the food, nutritional, cosmetic, pharmaceutical and nutraceutical industries. Bloom-forming cyanobacteria (Figure 1), for instance, such as Microcystis aeruginosa, Anabaena cylindrica and Nodularia harveyana, are known to produce a variety of bioactive metabolites.Among these metabolites, cyclic and linear peptides such as microcystin, nodularin, aeruginosin, anabaenopeptin, cyanopeptolin, microginin, cyclamides and microviridin Braz. Chem. Soc. 522 are found, which show activity against various proteases or protein phosphatases. 4 Most of these potent and "dangerous" peptides are released by the cyanobacteria cells into the environment and numerous cases of cyanobacteria poisoning in animals and humans have been reported worldwide.5 Some cyanobacteria also produce iron chelators known as siderophores such as anachelin, schizokinen and synechobactin. 6 There is an interesting behavior of these metabolites, among which the cyclic peptides microcystins and nodularins are such that they are retained inside the cell membrane, whereas anachelin finds its way out in the form of a siderophore, which passes through the membrane into and out of the cell.Mass spectrometry (MS) and mainly matrixassisted laser desorption ionization-mass spectrometry (MALDI-MS) Imaging mass spectrometry (IMS) that incorporates spatial distribution is an emerging and promising new technology for metabolomics MS analysis. This methodology was introduced in 1997 by Caprioli et al. 12 and mostly applied to create 2D images based on the spatial distribution of pe...

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“…This technique has a wide range of modifications, varying in application from a single cell to multiple colonies on a petri dish (reviewed by Watrous et al [57]). Interestingly, MS can be used in an imaging set-up to study metabolic interactions [58]. The potential of imaging-MS unfolded, for example, in a study of chemical interactions on actinomycete bacteria, showing interactions through spectra of secondary metabolites [59 ].…”

Section: Exploration Using Metabolomicsmentioning

confidence: 99%

Metabolic interactions in microbial communities: untangling the Gordian knot

Ponomarova

Patil

2015

Current Opinion in Microbiology

229

177

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Metabolic exchanges are ubiquitous in microbial communities. However, detecting metabolite cross-feedings is difficult due to their intrinsically dynamic nature and the complexity of communities. Thus, while exhaustive description of metabolic networks operating in natural systems is a task for the future, the battle of today is divided between detailed characterizations of small, reduced complexity microbial consortia, and focusing on particular metabolic aspects of natural ecosystems. Detecting metabolic interactions requires methodological blend able to capture species identity, dependencies and the nature of exchanged metabolites. Multiple combinations of diverse techniques, from metagenomics to imaging mass spectrometry, offer solutions to this challenge, each combination being tailored to the community at hand.

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Bringing microbial interactions to light using imaging mass spectrometry

Cited by 50 publications

References 111 publications

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

MALDI Imaging Mass Spectrometry of Fresh Water Cyanobacteria: Spatial Distribution of Toxins and Other Metabolites

Metabolic interactions in microbial communities: untangling the Gordian knot

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