“…The manufacturing process involves a preliminary operation of wet salting (brining), where traditionally, whole fish is immersed in saturated brine until osmotic equilibrium is achieved in the muscle. During this stage, water activity (a w ) is reduced from 0.99, corresponding to raw fish, to 0.80-0.84 (Filsinger, 1987;Czerner and Yeannes, 2010). Following this, anchovies are beheaded and partially gutted (leaving gonads and pyloric caeca), placed in barrels of alternating layers of fish and salt, and pressed, reducing the a w to 0.75 (Filsinger et al, 1987;Yeannes, 1996).…”