Broad-band fluorescence upconversion for femtosecond spectroscopy

Schanz, Roland; Kovalenko, Sergey A.; Kharlanov, V. A.; Érnsting, N. P.

doi:10.1063/1.1387257

Cited by 63 publications

(40 citation statements)

References 10 publications

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“…Hence, evolution such as shifting and narrowing can be more clearly observed without the need for spectral reconstruction techniques from the single-wavelength traces. [36,37] Recently, the upconversion technique has been expanded to include the near-simultaneous collection of multiple wavelengths, [38,39] which combines high time resolution (< 150 fs) with dispersed wavelength detection. Vengris et al used this technique to contrast the fluorescence dynamics in the PYP protein and in isolated model chromophores in solution.…”

Section: Time-resolved Fluorescence Signalsmentioning

confidence: 99%

Initial Photoinduced Dynamics of the Photoactive Yellow Protein

Larsen

Grondelle

2005

ChemPhysChem

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The photoactive yellow protein (PYP) is the photoreceptor protein responsible for initiating the blue-light repellent response of the Halorhodospira halophila bacterium. Optical excitation of the intrinsic chromophore in PYP, p-coumaric acid, leads to the initiation of a photocycle that comprises several distinct intermediates. The dynamical processes responsible for the initiation of the PYP photocycle have been explored with several time-resolved techniques, which include ultrafast electronic and vibrational spectroscopies. Ultrafast electronic spectroscopies, such as pump-visible probe, pump-dump-visible probe, and fluorescence upconversion, are useful in identifying the timescales and connectivity of the transient intermediates, while ultrafast vibrational spectroscopies link these intermediates to dynamic structures. Herein, we present the use of these techniques for exploring the initial dynamics of PYP, and show how these techniques provide the basis for understanding the complex relationship between protein and chromophore, which ultimately results in biological function.

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Section: Time-resolved Fluorescence Signalsmentioning

confidence: 99%

Initial Photoinduced Dynamics of the Photoactive Yellow Protein

Larsen

Grondelle

2005

ChemPhysChem

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“…[4][5][6][7] Fluorescence gating has been performed with a Kerr shutter, [8][9][10][11][12][13][14][15] by non-collinear optical parametric amplification [16][17][18][19][20] or by diffraction from a transient grating, 21 in addition to broadband upconversion schemes. [4][5][6][7][22][23][24][25] Here we consider only reports where spontaneous emission from molecular or similar sources is gated, dispersed, and registered simultaneously at all relevant wavelengths with a view to obtain true molecular spectra. Principal problems relate to the spectral bandwidth, the contrast (i.e., the number of gated photons from the sample relative to others), and to the time resolution.…”

Section: Introductionmentioning

confidence: 99%

Femtosecond broadband fluorescence upconversion spectroscopy: Spectral coverage versus efficiency

Gerecke

Bierhance

Gutmann³

et al. 2016

Review of Scientific Instruments

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Sum frequency mixing of fluorescence and ∼1300 nm gate pulses, in a thin β-barium borate crystal and non-collinear type II geometry, is quantified as part of a femtosecond fluorimeter [X.-X. Zhang et al., Rev. Sci. Instrum. 82, 063108 (2011)]. For a series of fixed phasematching angles, the upconversion efficiency is measured depending on fluorescence wavelength. Two useful orientations of the crystal are related by rotation around the surface normal. Orientation A has higher efficiency (factor ∼3) compared to B at the cost of some loss of spectral coverage for a given crystal angle. It should be used when subtle changes of an otherwise stationary emission band are to be monitored. With orientation B, the fluorescence range λ F > 420-750 nm is covered with a single setting of the crystal and less gate scatter around time zero. The accuracy of determining an instantaneous emission band shape is demonstrated by comparing results from two laboratories. Published by AIP Publishing. [http://dx

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“…This spectral correction method is different from that of femtosecond broadband fluorescence up-conversion spectroscopy. [10][11][12] The latter resorts to the steady-state fluorescence spectrum of the sample to be investigated. Regardless of its advantages, the performance of FNOPAS still requires further improvements in the following two aspects, i.e., (1) removal of the interference from the coherent photons; and (2) spectral correction of the transient fluorescence spectra for accurate measurement.…”

Section: Introductionmentioning

confidence: 99%

Coherent photon interference elimination and spectral correction in femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy

Dang

Mao

Weng

2013

Review of Scientific Instruments

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We report an improved setup of femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy (FNOPAS) with a 210 fs temporal response. The system employs a Cassegrain objective to collect and focus fluorescence photons, which eliminates the interference from the coherent photons in the fluorescence amplification by temporal separation of the coherent photons and the fluorescence photons. The gain factor of the Cassegrain objective-assisted FNOPAS is characterized as 1.24 × 10(5) for Rhodamine 6G. Spectral corrections have been performed on the transient fluorescence spectra of Rhodamine 6G and Rhodamine 640 in ethanol by using an intrinsic calibration curve derived from the spectrum of superfluorescence, which is generated from the amplification of the vacuum quantum noise. The validity of spectral correction is illustrated by comparisons of spectral shape and peak wavelength between the corrected transient fluorescence spectra of these two dyes acquired by FNOPAS and their corresponding standard reference spectra collected by the commercial streak camera. The transient fluorescence spectra of the Rhodamine 6G were acquired in an optimized phase match condition, which gives a deviation in the peak wavelength between the retrieved spectrum and the reference spectrum of 1.0 nm, while those of Rhodamine 640 were collected in a non-optimized phase match condition, leading to a deviation in a range of 1.0-3.0 nm. Our results indicate that the improved FNOPAS can be a reliable tool in the measurement of transient fluorescence spectrum for its high temporal resolution and faithfully corrected spectrum.

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Broad-band fluorescence upconversion for femtosecond spectroscopy

Cited by 63 publications

References 10 publications

Initial Photoinduced Dynamics of the Photoactive Yellow Protein

Initial Photoinduced Dynamics of the Photoactive Yellow Protein

Femtosecond broadband fluorescence upconversion spectroscopy: Spectral coverage versus efficiency

Coherent photon interference elimination and spectral correction in femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy

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