Broad Diversity of Fungi in Hospital Water

Arroyo, Máira Gazzola; Ferreira, Adriano Menis; Frota, Oleci Pereira; Brizzotti-Mazuchi, Natália Seron; Peresi, Jacqueline Tanury Mac Ruz; Rigotti, Marcelo Alessandro; MacEdo, Carlos Eduardo; Sousa, Álvaro Francisco Lopes de; Andrade, Denise de; Almeida, Margarete Teresa Gottardo de

doi:10.1155/2020/9358542

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…Aspergillus, Byssochlamys, Candida, Cephalosporium, Cladosporium, Fusarium, Mucor, Penicillium, Rhizopus, Saccharomyces, Sporobolomyces and Trichoderma were reported by Obire et al, (2009). Arroyo et al, (2020) also reported the presence of Aspergillus sp, Fusarium sp and Penicillium sp from hospital water which are also similar to the fungal isolates in the present study. Although in their study, Cladosporium sp was the most common fungal isolate with wide distribution followed by Penicillium sp while in the present study, Penicillium sp was the most common fungal isolates followed by Aspergillus.…”

Section: Resultssupporting

confidence: 89%

“…Pfaller and Diekema (2004) opined that these dematiaceous fungal isolates are the major cause of mycoses and other types of infections including cutaneous, subcutaneous, invasive, and contagious infections. More so, fungal genera such as Aspergillus, Fusarium, Mucor, Penicillium, etc are known mycotoxin producers (Prescott et al, 2011) and consumption of mycotoxins could lead to mycosis and vascular diseases (Arroyo et al, 2020). Most of the isolated fungal genera contain species that are potential pathogens or opportunistic pathogens.…”

Section: Resultsmentioning

confidence: 99%

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Effect of Human Activities on the Population and Distribution of Fungi in Lobia Creek in Bayelsa State, Nigeria

Flora¹,

Obire²

2022

Int.J.Curr.Microbiol.App.Sci

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Fungi are now gaining attention as a tool for water quality due to their implication in waterborne infections. The effect of human activities on the population, distribution and diversity of fungi in Lobia Creek was investigated. Water samples were collected from five designated stations along Lobia creek into separate sterile bottles. Samples were transported in ice packed coolers to the laboratory for analysis using standard mycological techniques. A total of 60 water samples were analyzed during the six months sampling period (August 2020 to January 2021). Results of fungal count for toilet, jetty, abattoir, drinking and control were 2.1±0.42×104cfu/ml, 1.1±0.14×104cfu/ml, 1.9±0.14×104cfu/ml, 7.0±0.04×102cfu/ml, and 7.0±0.01×102cfu/ml, respectively. The decreasing order of fungal population in the stations was; Toilet ˃ Abattoir ˃ Jetty ˃ Drinking water ˃ Control. Statistical analysis showed that, there was significant difference (p<0.05) across the sampled stations. Results also showed that the fungal counts recorded in the toilet station was not significantly different (P>0.05) from the counts recorded for the jetty and abattoir station despite being slightly higher but was significantly higher (P<0.05) than the counts recorded for drinking and control samples. Percentage occurrences for fungal isolates were: Rhizopus sp (2.3%), Penicillium sp (27.3%), Mucor sp (15.9%), Candida krusei (9.1%), A. niger (11.4%), Saccharomyces sp (4.5%), Fusarium sp (10.2%), Aspergillus sp (11.4%) and Candida sp (8.0%). The decreasing order of the types of fungi in the locations was; Toilet ˃ Abattoir ˃ Jetty ˃ Control ˃ Drinking water. The high fungal load and uneven distribution of fungal types in all stations is attributed to the type of human activity in respective station. The fungi reported are known potential pathogens that can cause respiratory, skin and soft tissue infections and poses health risk to the public.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Effect of Human Activities on the Population and Distribution of Fungi in Lobia Creek in Bayelsa State, Nigeria

Flora¹,

Obire²

2022

Int.J.Curr.Microbiol.App.Sci

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“…Regarding fungi, the ones we isolated in pure culture belonged to the genera Aureobasidium (five isolates), Ustilago , Cystobasidium , Rhodotorula and Cryptococcus (the last four represented by just one isolate). Despite the low number of fungal isolates selected, the fact that the yeast genera Aureobasidium , Rhodotorula and Cryptococcus had previously been reported to inhabit different hospital facilities is in accordance with our results 67 .…”

Section: Discussionsupporting

confidence: 91%

Ecology and resistance to UV light and antibiotics of microbial communities on UV cabins in the dermatology service of a Spanish hospital

Molina-Menor,

Carlotto,

Vidal-Verdú

et al. 2023

Sci Rep

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Microorganisms colonize all possible ecological habitats, including those subjected to harsh stressors such as UV radiation. Hospitals, in particular the UV cabins used in phototherapy units, constitute an environment in which microbes are intermittently subjected to UV irradiation. This selective pressure, in addition to the frequent use of antibiotics by patients, may represent a threat in the context of the increasing problem of antimicrobial resistance. In this work, a collection of microorganisms has been established in order to study the microbiota associated to the inner and outer surfaces of UV cabins and to assess their resistance to UV light and the antibiotics frequently used in the Dermatology Service of a Spanish hospital. Our results show that UV cabins harbor a relatively diverse biocenosis dominated by typically UV-resistant microorganisms commonly found in sun-irradiated environments, such as Kocuria, Micrococcus or Deinococcus spp., but also clinically relevant taxa, such as Staphylococcus or Pseudomonas spp. The UV-radiation assays revealed that, although some isolates displayed some resistance, UV is not a major factor shaping the biocenosis living on the cabins, since a similar pool of resistant microorganisms was identified on the external surface of the cabins. Interestingly, some Staphylococcus spp. displayed resistance to one or more antibiotics, although the hospital reported no cases of antibiotic-resistance infections of the patients using the cabins. Finally, no association between UV and antibiotic resistances was found.

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“…These outbreaks resulted in a total of 31 patient infections, with 3 fatalities. The primary cause of the outbreaks was contamination of the water supply pipes [ 10 ]. Some patients have died from infection with Legionella in the waterway during dental visits; a female patient succumbed to Legionnaires’ disease after receiving dental treatment.…”

Section: Introductionmentioning

confidence: 99%

Comparing the Effects of Two Culture Methods to Determine the Total Heterotrophic Bacterial Colony Count in Hospital Purified Water

Cao,

Xiong,

Fan

et al. 2024

J Epidemiol Glob Health

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Background Accurately detecting the quantity of microorganisms in hospital purified water is of significant importance for early identification of microbial contamination and reducing the occurrence of water-borne hospital infections. The choice of detection method is a prerequisite for ensuring accurate results. Traditional Plate Count Agar (PCA) belongs to a high-nutrient medium, and there may be limitations in terms of accuracy or sensitivity in detecting microorganisms in hospital purified water. On the other hand, Reasoner’s 2A agar (R2A) has characteristics, such as low-nutrient levels, low cultivation temperature, and extended incubation time, providing advantages in promoting the growth of aquatic microorganisms. This study, through comparing the differences in total colony counts between two detection methods, aims to select the method more suitable for the growth of aquatic microorganisms, offering new practical insights for accurately detecting the total count of heterotrophic bacteria in hospital purified water. Methods The most commonly used plate count agar (PCA) method, and the R2A agar culture were adopted to detect microorganisms and determine the total number of bacterial colonies in the water for oral diagnosis and treatment water and terminal rinse water for endoscopes in medical institutions. The two water samples were inoculated by pour plate and membrane filtration methods, respectively. Using statistical methods including Spearman and Pearson correlation, Wilcoxon signed-rank sum test, paired-Chi-square test, and linear regression, we analyze the differences and associations in the bacterial counts cultivated through two different methods. Results In 142 specimens of the water, the median and interquartile range of the heterotrophic bacterial colony number under the R2A culture method and under the PCA culture method were 200 (Q1–Q3: 25–18,000) and 6 (Q1–Q3: 0–3700). The total number of heterotrophic bacteria colonies cultured in R2A medium for 7 days was more than that cultured in PCA medium for 2 days (P < 0.05). The linear regression results showed a relatively strong linear correlation between the number of colonies cultured by the R2A method and that cultured by the PCA method (R2 = 0.7264). The number of bacterial species detected on R2A agar medium is greater than that on PCA agar medium. Conclusion The R2A culture method can better reflect the actual number of heterotrophic bacterial colonies in hospital purified water. After logarithmic transformation, the number of colonies cultured by the two methods showed a linear correlation.

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Broad Diversity of Fungi in Hospital Water

Cited by 10 publications

References 25 publications

Effect of Human Activities on the Population and Distribution of Fungi in Lobia Creek in Bayelsa State, Nigeria

Effect of Human Activities on the Population and Distribution of Fungi in Lobia Creek in Bayelsa State, Nigeria

Ecology and resistance to UV light and antibiotics of microbial communities on UV cabins in the dermatology service of a Spanish hospital

Comparing the Effects of Two Culture Methods to Determine the Total Heterotrophic Bacterial Colony Count in Hospital Purified Water

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