Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

Pelyuntha, Wattana; Sanguankiat, Arsooth; Kovitvadhi, Attawit; Vongkamjan, Kitiya

doi:10.14202/vetworld.2022.2039-2045

Cited by 10 publications

(11 citation statements)

References 54 publications

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“…Salmonella phage applications are already used in many countries in the food sector to avoid food contamination. Phage therapy in animal production is also reaching the stage of large-scale industrial trials [ 24 , 25 , 26 , 27 ]. However, these applications can be controversial, as phages drive bacterial evolution in Salmonella in the fastest and most effective way [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Salmonella Prophages, Their Propagation, Host Specificity and Antimicrobial Resistance Gene Transduction

et al. 2023

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Salmonella enterica subsp. enterica is a zoonotic bacterial pathogen that causes foodborne outbreaks in humans. Lytic bacteriophages to control Salmonella in food production are already being used in scientific studies and some are commercially available. However, phage application is still controversial. In addition to virulent phages, which are used in phage therapy and lyse the bacterial host, lysogenic phages coexist in the environment and can reside as prophages in the bacterial host. Therefore, information about Salmonella prophages is essential to understand successful phage therapy. In 100 Salmonella food isolates of the serovars Enteritidis and Typhimurium, we propagated prophages by oxidative stress. In isolates of the serovars Typhimurium and Enteritidis, 80% and 8% prophages could be activated, respectively. In the phage lysates from the serovar Typhimurium, the following antibiotic resistance genes or gene fragments were detected by PCR: sul1, sul2, blaTEM, strA and cmlA; however, no tetA,B,C, blaOXA, blaCMY, aadA1, dfr1,2 or cat were detected. In contrast, no resistance genes were amplified in the phage lysates of the serovar Enteritidis. None of the phage lysates was able to transduce phenotypic resistance to WT 14028s. Most of the prophage lysates isolated were able to infect the various Salmonella serovars tested. The high abundance of prophages in the genome of the serovar Typhimurium may counteract phage therapy through phage resistance and the development of hybrid phages.

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Section: Discussionmentioning

confidence: 99%

Salmonella Prophages, Their Propagation, Host Specificity and Antimicrobial Resistance Gene Transduction

et al. 2023

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“…Diverse serovars of Salmonella were recovered in the broiler production chain in that study, while the isolates presenting ciprofloxacin-resistant Salmonella were as high as 29.4%. Overall, Salmonella phages showed high lysis ability against these ciprofloxacin-resistant Salmonella isolates, suggesting the potential application of phage-based treatments or biocontrol in the broiler production chain ( Pelyuntha et al, 2022 ). Grabowski et al (2022) indicated for the first time that cocktail of phages targeting Salmonella is not only effective but also can be used in veterinary practices without disturbing immune homeostasis, expressed as cytokine imbalance, disturbed percentage of key immune cell subpopulations, and stress axis hyperactivity.…”

Section: Phage Therapy Of Bacterial Infections and Zoonotic Agents In...mentioning

confidence: 99%

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

et al. 2023

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The primary contaminants in poultry are Salmonella enterica, Campylobacter jejuni, Escherichia coli, and Staphylococcus aureus. Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses and poses a threat to public health. With the increasing prevalence of bacterial pathogens being resistant to most conventional antibiotics, scientists have rekindled interest in using bacteriophages as antimicrobial agents. Bacteriophage treatments have also been investigated as an alternative to antibiotics in the poultry industry. Bacteriophages’ high specificity may allow them only to target a specific bacterial pathogen in the infected animal. However, a tailor-made sophisticated cocktail of different bacteriophages could broaden their antibacterial activity in typical situations with multiple clinical strains infections. Bacteriophages may not only be used in terms of reducing bacterial contamination in animals but also, under industrial conditions, they can be used as safe disinfectants to reduce contamination on food-contact surfaces or poultry carcasses. Nevertheless, bacteriophage therapies have not been developed sufficiently for widespread use. Problems with resistance, safety, specificity, and long-term stability must be addressed in particular. This review highlights the benefits, challenges, and current limitations of bacteriophage applications in the poultry industry.

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“…The results of this study indicated that isolated phages could lyse (ranging from 33.3% to 93.3%) ciprofloxacin‐resistant bacteria. These data suggest that phages have a promising potential in combating ciprofloxacin‐resistant Salmonella isolates in the broiler production chain and preventing their spread through the food supply chain 2 . In another study, a Salmonella Typhi isolates with decreased susceptibility to ciprofloxacin and producing CTX‐M‐15 extended spectrum‐lactamase (ESBL) was isolated in the Democratic Republic of the Congo.…”

Section: The Use Of Phages For Ciprofloxacin‐resistant Bacteriamentioning

confidence: 99%

“…1 Ciprofloxacin resistance in different bacteria has been increasingly reported worldwide because of mutations in DNA gyrase genes (gyrA), efflux pumps, or plasmidmediated-quinolone resistance in bacterial plasmids or chromosomes. 2 In this regard, applying a combination of different substances such as nanoparticles, natural compounds, and bacteriophages with ciprofloxacin to enhance the efficacy of this antibiotic against multi-drug resistant (MDR) bacteria has received much attention. 3 Bacteriophages (phages) are viruses that could lyse bacteria while are not harmful to animal and human health.…”

Section: Introductionmentioning

confidence: 99%

“…This antibiotic suppresses the activities of DNA‐gyrase and DNA topoisomerase during DNA replication, recombination, and repair 1 . Ciprofloxacin resistance in different bacteria has been increasingly reported worldwide because of mutations in DNA gyrase genes (gyrA), efflux pumps, or plasmid‐mediated‐quinolone resistance in bacterial plasmids or chromosomes 2 . In this regard, applying a combination of different substances such as nanoparticles, natural compounds, and bacteriophages with ciprofloxacin to enhance the efficacy of this antibiotic against multi‐drug resistant (MDR) bacteria has received much attention 3 …”

Section: Introductionmentioning

confidence: 99%

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Bacteriophages: The promising therapeutic approach for enhancing ciprofloxacin efficacy against bacterial infection

Shariati,

Noei,

Chegini

2023

Clinical Laboratory Analysis

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Background The emergence of ciprofloxacin‐resistant bacteria is a serious challenge worldwide, bringing the need to find new approaches to manage this bacterium. Bacteriophages (phages) have been shown inhibitory effects against ciprofloxacin‐resistance bacteria; thus, ciprofloxacin resistance or tolerance may not affect the phage's infection ability. Additionally, researchers used phage‐ciprofloxacin combination therapy for the inhibition of multidrug‐resistant bacteria. Results The sublethal concentrations of ciprofloxacin could lead to an increase in progeny production. Antibiotic treatments could enhance the release of progeny phages by shortening the lytic cycle and latent period. Thus, sublethal concentrations of antibiotics combined with phages can be used for the management of bacterial infections with high antibiotic resistance. In addition, combination therapy exerts various selection pressures that can mutually decrease phage and antibiotic resistance. Moreover, phage ciprofloxacin could significantly reduce bacterial counts in the biofilm community. Immediate usage of phages after the attachment of bacteria to the surface of the flow cells, before the development of micro‐colonies, could lead to the best effect of phage therapy against bacterial biofilm. Noteworthy, phage should be used before antibiotics usage because this condition may have allowed phage replication to occur first before ciprofloxacin interrupted the bacterial DNA replication process, thereby interfering with the activity of the phages. Furthermore, the phage‐ciprofloxacin combination showed a promising result for the management of Pseudomonas aeruginosa infections in mouse models. Nevertheless, low data are existing about the interaction between phages and ciprofloxacin in combination therapies, especially regarding the emergence of phage‐resistant mutants. Additionally, there is a challenging and important question of how the combined ciprofloxacin with phages can increase antibacterial functions. Therefore, more examinations are required to support the clinical usage of phage‐ciprofloxacin combination therapy.

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Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

Cited by 10 publications

References 54 publications

Salmonella Prophages, Their Propagation, Host Specificity and Antimicrobial Resistance Gene Transduction

Salmonella Prophages, Their Propagation, Host Specificity and Antimicrobial Resistance Gene Transduction

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

Bacteriophages: The promising therapeutic approach for enhancing ciprofloxacin efficacy against bacterial infection

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