Broad range flavonoid profiling by LC/MS of soybean genotypes contrasting for resistance to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Gómez, Jenny D.; Vital, Camilo Elber; Oliveira, M. G. A.; Ramos, Humberto J.O.

doi:10.1371/journal.pone.0205010

Cited by 65 publications

(51 citation statements)

References 34 publications

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“…They were Tricin 4′- O -syringyl alcohol, Tricin O -rhamnosyl- O -malonylhexoside, Chrysoeriol O -hexosyl- O -pentoside, 8- C -hexosyl-chrysoeriol O -feruloylhexoside, Apigenin O -hexosyl- O -pentoside, Apigenin O -hexosyl- O -rutinoside, Chrysoeriol 5- O -hexoside, Daidzein 7- O -glucoside, Gossypol, Pedalitin, and Demethoxycurcumin. At the same time, Tricin 4′- O -syringyl alcohol was only detected in DHP, and the compounds Daidzein 7- O -glucoside and Demethoxycurcumin were reported to have pharmacological activities in the literature [34,35].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS

et al. 2019

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Flavonoids are a kind of essential substance for the human body because of their antioxidant properties and extremely high medicinal value. Citrus reticulata “Dahongpao” (DHP) is a special citrus variety that is rich in flavonoids, however little is known about its systematic flavonoids profile. In the present study, the presence of flavonoids in five important citrus varieties, including DHP, Citrus grandis Tomentosa (HZY), Citrus ichangensis Swingle (YCC), Citrus sinensis (L.) Osbeck (TC), and Citrus reticulata ‘Buzhihuo’ (BZH), was determined using a UPLC-ESI-MS/MS-based, widely targeted metabolome. Results showed that a total of 254 flavonoid metabolites (including 147 flavone, 39 flavonol, 21 flavanone, 24 anthocyanins, 8 isoflavone, and 15 polyphenol) were identified. The total flavonoid content of peels from DHP was the highest. DHP could be clearly separated from other samples through clustering analysis and principal component analysis (PCA). Further, 169 different flavonoid metabolites were observed between DHP peels and the other four citrus peels, and 26 down-regulated differential metabolites displayed important biological activities in DHP. At the same time, a unique flavonoid component, tricin 4′-O-syringyl alcohol, was only found in DHP, which could be used as a marker to distinguish between other varieties. This work might facilitate a better understanding of flavonoid metabolites between DHP peels and the other four citrus peels and provide a reference for its sufficient utilization in the future.

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Section: Discussionmentioning

confidence: 99%

Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS

et al. 2019

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“…At the metabolomic level, a metabolite associated with flavone and flavonol biosynthesis pathway, rutin, was significantly more abundant in ripe LR fruits than in LB fruits (5.88-fold). Increased rutin content was observed in plants in response to a drought stress 66 , salinity stress 67 , and also to biotic stress (pests) 68 . Intriguingly, this metabolite was not mentioned in a recent comparative metabolomic study of these two Lycium fruits 3 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

Zhao

Yin

et al. 2020

Sci Rep

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Red wolfberry (or goji berry, Lycium barbarum; LB) is an important agricultural product with a high content of pharmacologically important secondary metabolites such as phenylpropanoids. A close relative, black wolfberry (L. ruthenicum; LR), endemic to the salinized deserts of northwestern china, is used only locally. The two fruits exhibit many morphological and phytochemical differences, but genetic mechanisms underlying them remain poorly explored. in order to identify the genes of interest for further studies, we studied transcriptomic (Illumina HiSeq) and metabolomic (LC-MS) profiles of the two fruits during five developmental stages (young to ripe). As expected, we identified much higher numbers of significantly differentially regulated genes (DEGs) than metabolites. The highest numbers were identified in pairwise comparisons including the first stage for both species, but total numbers were consistently somewhat lower for the LR. The number of differentially regulated metabolites in pairwise comparisons of developmental stages varied from 66 (stages 3 vs 4) to 133 (stages 2 vs 5) in both species. We identified a number of genes (e.g. AAT1, metE, pip) and metabolites (e.g. rutin, raffinose, galactinol, trehalose, citrulline and DL-arginine) that may be of interest to future functional studies of stress adaptation in plants. As LB is also highly suitable for combating soil desertification and alleviating soil salinity/alkalinity/pollution, its potential for human use may be much wider than its current, highly localized, relevance.Despite their close phylogenetic relationship, the fruits of these two species exhibit distinct phenotypic profiles of during all developmental stages, including their shape, size, colour, taste, nutritional value and pharmacological properties 3,4,11 . As opposed to the red and elongated mature red wolfberry, black wolfberry is dark-purple or black, round, and smaller. It is also known that metabolic phenotypes of these two fruits differ significantly, particularly in the content of fatty acids, phenols and antioxidant capacities, which are much higher in black wolfberry, while the content of carotenoids, sugars, amino acids and osmolytes is higher in the red wolfberry 3,4 .Fruit ripening is a complex developmental process, coordinated by a network of interacting genes and signalling pathways 12 , so genetic mechanisms underpinning these phenotypic differences remain only partially understood. The objective of this study was to contribute to our understanding of the complexity of ripening processes of these two fruits in different environments. To achieve this, we collected L. barbarum and L. ruthenicum fruits at five developmental stages, from young to ripe fruit, and sequenced their transcriptomes and metabolomes. These data shall help us better understand genetic underpinnings of both within-and between-species phenotypic differences that fruits of these two species exhibit during their respective ripening processes. Materials and methodsSample collection. Fruits were collected ...

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“…Their contribution to growth is explained by their effect on auxin transport, shoot growth, root development and nitrogen fixing processes in legumes [46][47][48][49]. Examples of flavonoids in bean plants are kaempferol, quercetin [50,51], and rutin [52]. Flavonoids are also reported to mediate plant resistance to herbivores [53] thus, their increased occurrence could enhance defence against antagonists.…”

Section: Effect Of Treatments and Application Methods On Common Bean Mmentioning

confidence: 99%

Extracts of Common Pesticidal Plants Increase Plant Growth and Yield in Common Bean Plants

Mkindi

Tembo

Mbega

et al. 2020

Plants

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Common bean (Phaseolus vulgaris) is an important food and cash crop in many countries. Bean crop yields in sub-Saharan Africa are on average 50% lower than the global average, which is largely due to severe problems with pests and diseases as well as poor soil fertility exacerbated by low-input smallholder production systems. Recent on-farm research in eastern Africa has shown that commonly available plants with pesticidal properties can successfully manage arthropod pests. However, reducing common bean yield gaps still requires further sustainable solutions to other crop provisioning services such as soil fertility and plant nutrition. Smallholder farmers using pesticidal plants have claimed that the application of pesticidal plant extracts boosts plant growth, potentially through working as a foliar fertiliser. Thus, the aims of the research presented here were to determine whether plant growth and yield could be enhanced and which metabolic processes were induced through the application of plant extracts commonly used for pest control in eastern Africa. Extracts from Tephrosia vogelii and Tithonia diversifolia were prepared at a concentration of 10% w/v and applied to potted bean plants in a pest-free screen house as foliar sprays as well as directly to the soil around bean plants to evaluate their contribution to growth, yield and potential changes in primary or secondary metabolites. Outcomes of this study showed that the plant extracts significantly increased chlorophyll content, the number of pods per plant and overall seed yield. Other increases in metabolites were observed, including of rutin, phenylalanine and tryptophan. The plant extracts had a similar effect to a commercially available foliar fertiliser whilst the application as a foliar spray was better than applying the extract to the soil. These results suggest that pesticidal plant extracts can help overcome multiple limitations in crop provisioning services, enhancing plant nutrition in addition to their established uses for crop pest management.

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Broad range flavonoid profiling by LC/MS of soybean genotypes contrasting for resistance to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Cited by 65 publications

References 34 publications

Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS

Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

Extracts of Common Pesticidal Plants Increase Plant Growth and Yield in Common Bean Plants

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