Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Kuan, Yi-Chia; Venkatesan, T.; Lin, Jia-Shin; Liu, Jia-Hsin; Chen, Tsan‑Jan; Wu, Hsin‐Mao; Wang, Wen‑Ching; Chen, Liang‐Jwu

doi:10.1007/s11816-018-0469-8

Cited by 2 publications

(1 citation statement)

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“…Since plant biosystems design may require multiple iterations of Design-Build-Test-Learn (DBTL) cycles (for details see Section 3 ), it is essential that the genetically modified plants or de novo plant systems can be easily upgraded for improving performance or adding new functions. In general, upgrading the plant genome requires consecutive stable plant transformation processes, which is constrained by a limited number of selectable marker genes available for plant transformation, including widely used selectable marker genes conferring antibiotic (e.g., kanamycin and hygromycin) or herbicide (e.g., BASTA) resistance [ 65 ], along with some nonantibiotic and nonherbicide markers such as plant phosphomannose isomerase [ 66 ], broad-specificity amino acid racemase [ 67 ], and fluorescent proteins [ 68 , 69 ]. For enabling upgradability of plant biosystems design, it would be desirable to consider marker-free plant transformation systems, in which the selectable marker gene can be excised from the plant genome after transformation (Figure 4 (e)).…”

Section: Theoretical Approaches and Principles Of Plant Biosystems De...mentioning

confidence: 99%

Plant Biosystems Design Research Roadmap 1.0

et al. 2020

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Human life intimately depends on plants for food, biomaterials, health, energy, and a sustainable environment. Various plants have been genetically improved mostly through breeding, along with limited modification via genetic engineering, yet they are still not able to meet the ever-increasing needs, in terms of both quantity and quality, resulting from the rapid increase in world population and expected standards of living. A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches. This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems. Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes. From this perspective, we present a comprehensive roadmap of plant biosystems design covering theories, principles, and technical methods, along with potential applications in basic and applied plant biology research. We highlight current challenges, future opportunities, and research priorities, along with a framework for international collaboration, towards rapid advancement of this emerging interdisciplinary area of research. Finally, we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception, trust, and acceptance.

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