Broad‐spectrum cytotoxicity to cancer cells of Brevilaterin C from <i>Brevibacillus laterosporus</i> and its specific mechanism on human epidermal cancer cells

Zhou, C.; Wang, Lulu; Hong, Dan; Liu, Yangliu; Han, Panpan; Li, Siting; Jia, Yingmin

doi:10.1002/jcb.30280

Cited by 5 publications

(12 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we aimed to investigate the impact of BB/BC, a spectrum anticancer peptide [ 14 , 15 ], on gastric cancer cells. Our results showed that BB/BC strongly inhibited cell proliferation in a dose-dependent manner ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The cell lines tested in this study included BGC-823 gastric cancer cells and its corresponding gastric epithelial cell GES-1, which is a human normal cell. BGC-823 and GES-1 cell lines were cultured using RPMI 1640 medium supplemented with 10% FBS and 1% penicillin-streptomycin cocktail, and cultured at 37 °C in a humidified atmosphere containing 5% CO 2 , as described previously [ 14 ]. FBS, penicillin, and cultural media were all purchased from Gibco (Oklahoma, ME, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability was assessed using the CCK-8 Assay Kit, following the previously described protocol [ 14 ]. Both BGC-823 and GES-1 cells were in the logarithmic growth phase, with a concentration of 5 × 10 4 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

“…To assess apoptosis in BGC-823, we measured apoptotic rate, changes in ROS levels, and mitochondrial membrane potential using the Annexin V-FITC/PI Kit, DCFH-DA, and JC-1 fluorescent probes, respectively. The methods employed were previously described by Chen et al [ 14 ]. Specifically, logarithmically growing cells (2 × 10 5 cells/mL) were cultured for 24 h, and then treated with various concentrations of Brevilaterins (0, 4, 6, 8 µg/mL) for an additional 24 h. To determine apoptotic rate, 100 µL of treated cells were mixed with 5 µL of Annexin V-FITC for 10 min, followed by the addition of 5 µL PI.…”

Section: Methodsmentioning

confidence: 99%

“…These bacteria cover the majority of representative food-borne pathogens and spoilage bacteria commonly found in different foods [ 13 ]. Furthermore, our previous investigations have also revealed that Brevilaterins have the potential to effectively inhibit the proliferation of 27 human cancer cell lines, with a particularly strong inhibitory effect against gastric cancer cells [ 14 , 15 ]. However, the detailed anticancer characteristics and mechanisms of Brevilaterins against gastric cancer cells remain unclear and warrant further investigation.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Novel Property Cytotoxicity and Mechanism of Food Preservative Brevilaterins against Human Gastric Cancer Cells

Zhou

Hong

et al. 2023

Foods

Self Cite

View full text Add to dashboard Cite

Brevilaterins, antimicrobial peptides produced by Brevibacillus laterosporus, are regarded as excellent food preservatives and are popular as antimicrobial applications. Recent research has uncovered their potent cytotoxic effects against diverse cancer cells, thereby underscoring the pressing need for more extensive and intensive investigations into this use. In this study, we explored their novel function in inducing cytotoxicity to cancer cells and systematically investigated the mechanism of action of Brevilaterin B/C (BB/BC) in vivo. Proliferation, membrane permeability, and apoptotic rate were evaluated using CCK-8 assay, LDH assay, and Annexin V-FITC/PI kits. ROS levels and mitochondrial membrane potential were detected using the fluorescent probe DCFH-DA and JC-1. Our results demonstrated that both BB and BC at concentrations of 4–6 µg/mL significantly inhibited the proliferation and migration of gastric cancer cells BGC-823. Treatment with 4 µg/mL of BB/BC rapidly increased LDH levels in the supernatant of BGC-823 cells, leading to further investigation of the mechanism of apoptosis. We found that the apoptotic rate of BGC-823 cells significantly increased upon treatment with BB/BC, demonstrating their potent induction of apoptosis. BB/BC-induced ROS production in BGC-823 cells impaired their growth and induced apoptosis, indicating a close association between apoptosis and ROS elevation. Additionally, JC-1 aggregates rapidly accumulated after treatment with 4 µg/mL of BB/BC, suggesting changes in mitochondrial membrane potential and early apoptosis. Taken together, our findings revealed that BB and BC exhibit significant anticancer effects against gastric cancer cells, highlighting the promising potential of Brevilaterins as anticancer agents.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel Property Cytotoxicity and Mechanism of Food Preservative Brevilaterins against Human Gastric Cancer Cells

Zhou

Hong

et al. 2023

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

The antibacterial activity and mechanism of a novel peptide MR-22 against multidrug-resistant Escherichia coli

Tian,

Zhao,

Yang

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionBacterial infections have become serious threats to human health, and the excessive use of antibiotics has led to the emergence of multidrug-resistant (MDR) bacteria. E. coli is a human bacterial pathogen, which can cause severe infectious. Antimicrobial peptides are considered the most promising alternative to traditional antibiotics.Materials and methodsThe minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and hemolytic activity were determined by the microdilution method. The antimicrobial kinetics of MR-22 against E. coli were studied by growth curves and time-killing curves. The cytotoxicity of MR-22 was detected by the CCK-8 assay. The antimicrobial activity of MR-22 in salt, serum, heat and trypsin was determined by the microdilution method. The antimicrobial mechanism of MR-22 against drug-resistant E. coli was studied by Scanning Electron Microscope, laser confocal microscopy, and Flow Cytometry. The in vivo antibacterial activity of MR-22 was evaluated by the mice model of peritonitis.Results and discussionIn this study, MR-22 is a new antimicrobial peptide with good activity that has demonstrated against MDR E. coli. The antimicrobial activity of MR-22 exhibited stability under conditions of high temperature, 10% FBS, and Ca2+. However, a decline of the activity was observed in the presence of Na+, serum, and trypsin. MR-22 had no significant cytotoxicity or hemolysis in vitro. SEM and fluorescent images revealed that MR-22 could disrupt the integrity of cell membrane. DCFH-DA indicated that MR-22 increased the content of reactive oxygen species, while it decreased the content of intracellular ATP. In mice model of peritonitis, MR-22 exhibited potent antibacterial activity in vivo. These results indicated that MR-22 is a potential drug candidate against drug-resistant E. coli.

show abstract

Bacteriocins in Cancer Treatment: Mechanisms and Clinical Potentials

Wang,

Sun

et al. 2024

Biomolecules

View full text Add to dashboard Cite

Cancer poses a severe threat to human health. Although conventional chemotherapy remains a cornerstone of cancer treatment, its significant side effects and the growing issue of drug resistance necessitate the urgent search for more efficient and less toxic anticancer drugs. In recent years, bacteriocins, antimicrobial peptides of microbial origin, have garnered significant attention due to their targeted antitumor activity. This unique activity is mainly attributed to their cationic and amphiphilic nature, which enables bacteriocins to specifically kill tumor cells without harming normal cells. When involving non-membrane-disrupting mechanisms, such as apoptosis induction, cell cycle blockade, and metastasis inhibition, the core mechanism of action is achieved by disrupting cell membranes, which endows bacteriocins with low drug resistance and high selectivity. However, the susceptibility of bacteriocins to hydrolysis and hemolysis in vivo limits their clinical application. To overcome these challenges, structural optimization of bacteriocins or their combination with nanotechnology is proposed for future development. This review aims to study the mechanism of action and current research status of bacteriocins as anticancer treatments, thus providing new insights for their clinical development and application.

show abstract

Broad‐spectrum cytotoxicity to cancer cells of Brevilaterin C from Brevibacillus laterosporus and its specific mechanism on human epidermal cancer cells

Cited by 5 publications

References 36 publications

Novel Property Cytotoxicity and Mechanism of Food Preservative Brevilaterins against Human Gastric Cancer Cells

Novel Property Cytotoxicity and Mechanism of Food Preservative Brevilaterins against Human Gastric Cancer Cells

The antibacterial activity and mechanism of a novel peptide MR-22 against multidrug-resistant Escherichia coli

Bacteriocins in Cancer Treatment: Mechanisms and Clinical Potentials

Contact Info

Product

Resources

About