Broad-spectrum resistance against multiple PVY-strains by CRSIPR/Cas13 system in <i>Solanum tuberosum</i> crop

Noureen, Azka; Khan, Muhammad Zuhaib; Amin, Imran; Zainab, Tayyaba; Ahmad, Nasim; Haider, Sibtain; Mansoor, Shahid

doi:10.1080/21645698.2022.2080481

Cited by 15 publications

(4 citation statements)

References 53 publications

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“…Furthermore, the pre-CRISPR RNA was processed by Cas13 (due to its innate ability) into functional CRISPR RNA to target many viral mRNAs simultaneously. This may provide an alternative system to improve its efficiency distinctly [ 92 , 97 , 137 ]. A second strategy is to achieve viral resistance by editing the specific plant genes that are responsible for virus resistance traits [ 52 , 95 , 115 ].…”

Section: Crispr/cas Technique For Disease Resistancementioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game-changing tool that can create variation in desired traits, such as biotic and abiotic resistance, increase quality and yield in less time with easy applications, high efficiency, and low cost in producing the targeted edits for rapid improvement of crop plants. Plant pathogens and the severe environment cause considerable crop losses worldwide. GE approaches have emerged and opened new doors for breeding multiple-resistance crop varieties. Here, we have summarized recent advances in CRISPR/Cas-mediated GE for resistance against biotic and abiotic stresses in a crop molecular breeding program that includes the modification and improvement of genes response to biotic stresses induced by fungus, virus, and bacterial pathogens. We also discussed in depth the application of CRISPR/Cas for abiotic stresses (herbicide, drought, heat, and cold) in plants. In addition, we discussed the limitations and future challenges faced by breeders using GE tools for crop improvement and suggested directions for future improvements in GE for agricultural applications, providing novel ideas to create super cultivars with broad resistance to biotic and abiotic stress.

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Section: Crispr/cas Technique For Disease Resistancementioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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“…oryzae (Xoo) by disturbing effector binding elements (EBEs) in the promoter region of the OsSWEET14 gene [ 20 ]. Similarly, a CRISPR-Cas13-mediated multiplexing approach has been used in potatoes to confer resistance to multiple strains of potato virus Y (PVY) [ 21 ]. For wheat, the phytic acid content has been decreased to biofortify the crop by enhancing the accumulation of iron and zinc in mature grains [ 22 ].…”

Section: The Policies and Regulation Of Gene-editing In Asia And Aust...mentioning

confidence: 99%

Enabling Trade in Gene-Edited Produce in Asia and Australasia: The Developing Regulatory Landscape and Future Perspectives

Jones

Fosu-Nyarko

Iqbal

et al. 2022

Plants

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Genome- or gene-editing (abbreviated here as ‘GEd’) presents great opportunities for crop improvement. This is especially so for the countries in the Asia-Pacific region, which is home to more than half of the world’s growing population. A brief description of the science of gene-editing is provided with examples of GEd products. For the benefits of GEd technologies to be realized, international policy and regulatory environments must be clarified, otherwise non-tariff trade barriers will result. The status of regulations that relate to GEd crop products in Asian countries and Australasia are described, together with relevant definitions and responsible regulatory bodies. The regulatory landscape is changing rapidly: in some countries, the regulations are clear, in others they are developing, and some countries have yet to develop appropriate policies. There is clearly a need for the harmonization or alignment of GEd regulations in the region: this will promote the path-to-market and enable the benefits of GEd technologies to reach the end-users.

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“…All of them were transformed by double-stranded sequences transcribing the coat protein, replicase, or helicase of the pathogenic viruses themselves for RNAi [94,[100][101][102]. In recent years, clustered regularly interspaced short palindromic repeats-cas (CRISPR/Cas) technology and its new advances attracted much attention in transgenic improvement for rival resistance [103][104][105][106]. Transgenic potato lines with broad-spectrum resistance were developed by introduction of a multiple gRNA cassette of CRSIPR/Cas13 system to target six genes of three potato virus strains [106].…”

Section: Resistance To Pathogenic Virusesmentioning

confidence: 99%

“…This system can precisely target plant RNA viruses to impart resistance, and even can be multiplexed by designing a streamlined multiple gRNA cassette [105,245]. By this strategy, three transgenic potato lines with broad-spectrum resistance were developed by the introduction of a multiple gRNA cassette of CRSIPR/Cas13 system to target six genes of three potato virus strains [106].…”

Section: Gene Editionmentioning

confidence: 99%

Transgenic Improvement for Biotic Resistance of Crops

Wang

et al. 2022

IJMS

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Biotic constraints, including pathogenic fungi, viruses and bacteria, herbivory insects, as well as parasitic nematodes, cause significant yield loss and quality deterioration of crops. The effect of conventional management of these biotic constraints is limited. The advances in transgenic technologies provide a direct and directional approach to improve crops for biotic resistance. More than a hundred transgenic events and hundreds of cultivars resistant to herbivory insects, pathogenic viruses, and fungi have been developed by the heterologous expression of exogenous genes and RNAi, authorized for cultivation and market, and resulted in a significant reduction in yield loss and quality deterioration. However, the exploration of transgenic improvement for resistance to bacteria and nematodes by overexpression of endogenous genes and RNAi remains at the testing stage. Recent advances in RNAi and CRISPR/Cas technologies open up possibilities to improve the resistance of crops to pathogenic bacteria and plant parasitic nematodes, as well as other biotic constraints.

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Broad-spectrum resistance against multiple PVY-strains by CRSIPR/Cas13 system in Solanum tuberosum crop

Cited by 15 publications

References 53 publications

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Enabling Trade in Gene-Edited Produce in Asia and Australasia: The Developing Regulatory Landscape and Future Perspectives

Transgenic Improvement for Biotic Resistance of Crops

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